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  • Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-04
    Amber N. Stratman; Margaret C. Burns; Olivia M. Farrelly; Andrew E. Davis; Wenling Li; Van N. Pham; Daniel Castranova; Joseph J. Yano; Lauren M. Goddard; Oliver Nguyen; Marina Venero Galanternik; Timothy J. Bolan; Mark L. Kahn; Yoh-suke Mukouyama; Brant M. Weinstein

    The preferential accumulation of vascular smooth muscle cells (vSMCs) on arteries versus veins during early development is a well-described phenomenon, but the molecular pathways underlying this polarization are not well understood. In zebrafish, the cxcr4a receptor (mammalian CXCR4) and its ligand cxcl12b (mammalian CXCL12) are both preferentially expressed on arteries at time points consistent with

    更新日期:2020-12-04
  • Individual variability in functional connectivity architecture of the mouse brain
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-04
    Eyal Bergmann; Xenia Gofman; Alexandra Kavushansky; Itamar Kahn

    In recent years precision fMRI has emerged in human brain research, demonstrating characterization of individual differences in brain organization. However, mechanistic investigations to the sources of individual variability are limited in humans and thus require animal models. Here, we used resting-state fMRI in awake mice to quantify the contribution of individual variation to the functional architecture

    更新日期:2020-12-04
  • Cyanobacterial blooms contribute to the diversity of antibiotic-resistance genes in aquatic ecosystems
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-04
    Qi Zhang; Zhenyan Zhang; Tao Lu; W. J. G. M. Peijnenburg; Michael Gillings; Xiaoru Yang; Jianmeng Chen; Josep Penuelas; Yong-Guan Zhu; Ning-Yi Zhou; Jianqiang Su; Haifeng Qian

    Cyanobacterial blooms are a global ecological problem that directly threatens human health and crop safety. Cyanobacteria have toxic effects on aquatic microorganisms, which could drive the selection for resistance genes. The effect of cyanobacterial blooms on the dispersal and abundance of antibiotic-resistance genes (ARGs) of concern to human health remains poorly known. We herein investigated the

    更新日期:2020-12-04
  • Transfer learning efficiently maps bone marrow cell types from mouse to human using single-cell RNA sequencing
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-04
    Patrick S. Stumpf; Xin Du; Haruka Imanishi; Yuya Kunisaki; Yuichiro Semba; Timothy Noble; Rosanna C. G. Smith; Matthew Rose-Zerili; Jonathan J. West; Richard O. C. Oreffo; Katayoun Farrahi; Mahesan Niranjan; Koichi Akashi; Fumio Arai; Ben D. MacArthur

    Biomedical research often involves conducting experiments on model organisms in the anticipation that the biology learnt will transfer to humans. Previous comparative studies of mouse and human tissues were limited by the use of bulk-cell material. Here we show that transfer learning—the branch of machine learning that concerns passing information from one domain to another—can be used to efficiently

    更新日期:2020-12-04
  • The substrate specificity of the human TRAPPII complex’s Rab-guanine nucleotide exchange factor activity
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-04
    Meredith L. Jenkins; Noah J. Harris; Udit Dalwadi; Kaelin D. Fleming; Daniel S. Ziemianowicz; Atefeh Rafiei; Emily M. Martin; David C. Schriemer; Calvin K. Yip; John E. Burke

    The TRAnsport Protein Particle (TRAPP) complexes act as Guanine nucleotide exchange factors (GEFs) for Rab GTPases, which are master regulators of membrane trafficking in eukaryotic cells. In metazoans, there are two large multi-protein TRAPP complexes: TRAPPII and TRAPPIII, with the TRAPPII complex able to activate both Rab1 and Rab11. Here we present detailed biochemical characterisation of Rab-GEF

    更新日期:2020-12-04
  • Reactivation during sleep with incomplete reminder cues rather than complete ones stabilizes long-term memory in humans
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-04
    Cecilia Forcato; Jens G. Klinzing; Julia Carbone; Michael Radloff; Frederik D. Weber; Jan Born; Susanne Diekelmann

    Reactivation by reminder cues labilizes memories during wakefulness, requiring reconsolidation to persist. In contrast, during sleep, cued reactivation seems to directly stabilize memories. In reconsolidation, incomplete reminders are more effective in reactivating memories than complete reminders by inducing a mismatch, i.e. a discrepancy between expected and actual events. Whether mismatch is likewise

    更新日期:2020-12-04
  • The intracellular lipid-binding domain of human Na + /H + exchanger 1 forms a lipid-protein co-structure essential for activity
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-03
    Ruth Hendus-Altenburger; Jens Vogensen; Emilie Skotte Pedersen; Alessandra Luchini; Raul Araya-Secchi; Anne H. Bendsoe; Nanditha Shyam Prasad; Andreas Prestel; Marité Cardenas; Elena Pedraz-Cuesta; Lise Arleth; Stine F. Pedersen; Birthe B. Kragelund

    Dynamic interactions of proteins with lipid membranes are essential regulatory events in biology, but remain rudimentarily understood and particularly overlooked in membrane proteins. The ubiquitously expressed membrane protein Na+/H+-exchanger 1 (NHE1) regulates intracellular pH (pHi) with dysregulation linked to e.g. cancer and cardiovascular diseases. NHE1 has a long, regulatory cytosolic domain

    更新日期:2020-12-03
  • Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-03
    Peiwen Wang; Yuying Wang; Weihao Wang; Tong Chen; Shiping Tian; Guozheng Qin

    Carotenoids are natural pigments that are indispensable to plants and humans, whereas the regulation of carotenoid biosynthesis by post-translational modification remains elusive. Here, we show that a tomato E3 ubiquitin ligase, Plastid Protein Sensing RING E3 ligase 1 (PPSR1), is responsible for the regulation of carotenoid biosynthesis. PPSR1 exhibits self-ubiquitination activity and loss of PPSR1

    更新日期:2020-12-03
  • Sustained blood glutamate scavenging enhances protection in ischemic stroke
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-03
    Ahlem Zaghmi; Antonio Dopico-López; María Pérez-Mato; Ramón Iglesias-Rey; Pablo Hervella; Andrea A. Greschner; Ana Bugallo-Casal; Andrés da Silva; María Gutiérrez-Fernández; José Castillo; Francisco Campos Pérez; Marc A. Gauthier

    Stroke is a major cause of morbidity, mortality, and disability. During ischemic stroke, a marked and prolonged rise of glutamate concentration in the brain causes neuronal cell death. This study explores the protective effect of a bioconjugate form of glutamate oxaloacetate transaminase (hrGOT), which catalyzes the depletion of blood glutamate in the bloodstream for ~6 days following a single administration

    更新日期:2020-12-03
  • A C21-steroidal derivative suppresses T-cell lymphoma in mice by inhibiting SIRT3 via SAP18-SIN3
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-03
    Babu Gajendran; Krishnapriya M. Varier; Wuling Liu; Chunlin Wang; Klarke M. Sample; Eldad Zacksenhaus; Cui Juiwei; LieJun Huang; XiaoJiang Hao; Yaacov Ben-David

    The SIN3 repressor complex and the NAD-dependent deacetylase SIRT3 control cell growth, and development as well as malignant transformation. Even then, a little known about cross-talks between these two chromatin modifiers or whether their interaction explored therapeutically. Here we describe the identification of a C21-steroidal derivative compound, 3-O-chloroacetyl-gagamine, A671, which potently

    更新日期:2020-12-03
  • Author Correction: A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-02
    Sissi Miguel; Guillaume Legrand; Léonor Duriot; Marianne Delporte; Barbara Menin; Cindy Michel; Alexandre Olry; Gabrielle Chataigné; Aleksander Salwinski; Joakim Bygdell; Dominique Vercaigne; Gunnar Wingsle; Jean Louis Hilbert; Frédéric Bourgaud; Alain Hehn; David Gagneul

    A Correction to this paper has been published: https://doi.org/10.1038/s42003-020-01488-x

    更新日期:2020-12-02
  • Multiple proteases are involved in mesothelin shedding by cancer cells
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-01
    Xiufen Liu; Alexander Chan; Chin-Hsien Tai; Thorkell Andresson; Ira Pastan

    Mesothelin (MSLN) is a lineage restricted cell surface protein expressed in about 30% of human cancers and high MSLN expression is associated with poor survival in several different cancers. The restricted expression of MSLN in normal tissue and its frequent expression in cancers make MSLN an excellent target for antibody-based therapies. Many clinical trials with agents targeting MSLN have been carried

    更新日期:2020-12-01
  • Modeling the effects of perisaccadic attention on gaze statistics during scene viewing
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-01
    Lisa Schwetlick; Lars Oliver Martin Rothkegel; Hans Arne Trukenbrod; Ralf Engbert

    How we perceive a visual scene depends critically on the selection of gaze positions. For this selection process, visual attention is known to play a key role in two ways. First, image-features attract visual attention, a fact that is captured well by time-independent fixation models. Second, millisecond-level attentional dynamics around the time of saccade drives our gaze from one position to the

    更新日期:2020-12-01
  • Plasmodium falciparum sexual parasites regulate infected erythrocyte permeability
    Commun. Biol. (IF 4.165) Pub Date : 2020-12-01
    Guillaume Bouyer; Daniela Barbieri; Florian Dupuy; Anthony Marteau; Abdoulaye Sissoko; Marie-Esther N’Dri; Gaelle Neveu; Laurianne Bedault; Nabiha Khodabux; Diana Roman; Sandrine Houzé; Giulia Siciliano; Pietro Alano; Rafael M. Martins; Jose-Juan Lopez-Rubio; Jérome Clain; Romain Duval; Stéphane Egée; Catherine Lavazec

    To ensure the transport of nutrients necessary for their survival, Plasmodium falciparum parasites increase erythrocyte permeability to diverse solutes. These new permeation pathways (NPPs) have been extensively characterized in the pathogenic asexual parasite stages, however the existence of NPPs has never been investigated in gametocytes, the sexual stages responsible for transmission to mosquitoes

    更新日期:2020-12-01
  • Lysine demethylase 7a regulates murine anterior-posterior development by modulating the transcription of Hox gene cluster
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-30
    Yoshiki Higashijima; Nao Nagai; Masamichi Yamamoto; Taro Kitazawa; Yumiko K. Kawamura; Akashi Taguchi; Natsuko Nakada; Masaomi Nangaku; Tetsushi Furukawa; Hiroyuki Aburatani; Hiroki Kurihara; Youichiro Wada; Yasuharu Kanki

    Temporal and spatial colinear expression of the Hox genes determines the specification of positional identities during vertebrate development. Post-translational modifications of histones contribute to transcriptional regulation. Lysine demethylase 7A (Kdm7a) demethylates lysine 9 or 27 di-methylation of histone H3 (H3K9me2, H3K27me2) and participates in the transcriptional activation of developmental

    更新日期:2020-12-01
  • An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Amir Momen-Roknabadi; Panos Oikonomou; Maxwell Zegans; Saeed Tavazoie

    Genome-scale CRISPR interference (CRISPRi) is widely utilized to study cellular processes in a variety of organisms. Despite the dominance of Saccharomyces cerevisiae as a model eukaryote, an inducible genome-wide CRISPRi library in yeast has not yet been presented. Here, we present a genome-wide, inducible CRISPRi library, based on spacer design rules optimized for S. cerevisiae. We have validated

    更新日期:2020-11-27
  • Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Johann Peltier; Audrey Hamiot; Julian R. Garneau; Pierre Boudry; Anna Maikova; Eliane Hajnsdorf; Louis-Charles Fortier; Bruno Dupuy; Olga Soutourina

    Toxin-antitoxin (TA) systems are widespread on mobile genetic elements and in bacterial chromosomes. In type I TA, synthesis of the toxin protein is prevented by the transcription of an antitoxin RNA. The first type I TA were recently identified in the human enteropathogen Clostridioides difficile. Here we report the characterization of five additional type I TA within phiCD630-1 (CD0977.1-RCd11, CD0904

    更新日期:2020-11-27
  • Ligand-induced conformational rearrangements regulate the switch between membrane-proximal and distal functions of Rho kinase 2
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    István Hajdú; András Szilágyi; Barbara M. Végh; András Wacha; Dániel Györffy; Éva Gráczer; Márk Somogyi; Péter Gál; Péter Závodszky

    Rho-associated protein kinase 2 (ROCK2) is a membrane-anchored, long, flexible, multidomain, multifunctional protein. Its functions can be divided into two categories: membrane-proximal and membrane-distal. A recent study concluded that membrane-distal functions require the fully extended conformation, and this conclusion was supported by electron microscopy. The present solution small-angle X-ray

    更新日期:2020-11-27
  • Aberrant (pro)renin receptor expression induces genomic instability in pancreatic ductal adenocarcinoma through upregulation of SMARCA5/SNF2H
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Yuki Shibayama; Kazuo Takahashi; Hisateru Yamaguchi; Jun Yasuda; Daisuke Yamazaki; Asadur Rahman; Takayuki Fujimori; Yoshihide Fujisawa; Shinji Takai; Toru Furukawa; Tsutomu Nakagawa; Hiroyuki Ohsaki; Hideki Kobara; Jing Hao Wong; Tsutomu Masaki; Yukio Yuzawa; Hideyasu Kiyomoto; Shinichi Yachida; Akihiro Fujimoto; Akira Nishiyama

    (Pro)renin receptor [(P)RR] has a role in various diseases, such as cardiovascular and renal disorders and cancer. Aberrant (P)RR expression is prevalent in pancreatic ductal adenocarcinoma (PDAC) which is the most common pancreatic cancer. Here we show whether aberrant expression of (P)RR directly leads to genomic instability in human pancreatic ductal epithelial (HPDE) cells. (P)RR-expressing HPDE

    更新日期:2020-11-27
  • Inhibition of the CCL2 receptor, CCR2, enhances tumor response to immune checkpoint therapy
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Megan M. Tu; Hany A. Abdel-Hafiz; Robert T. Jones; Annie Jean; Katelyn J. Hoff; Jason E. Duex; Ana Chauca-Diaz; James C. Costello; Garrett M. Dancik; Beth A. Jirón Tamburini; Bogdan Czerniak; Jonathan Kaye; Dan Theodorescu

    Immunotherapies targeting the PD-1/PD-L1 axis are now a mainstay in the clinical management of multiple cancer types, however, many tumors still fail to respond. CCL2 is highly expressed in various cancer types and has been shown to be associated with poor prognosis. Inhibition or blockade of the CCL2/CCR2 signaling axis has thus been an area of interest for cancer therapy. Here we show across multiple

    更新日期:2020-11-27
  • A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Giorgia Manzo; Charlotte K. Hind; Philip M. Ferguson; Richard T. Amison; Alice C. Hodgson-Casson; Katarzyna A. Ciazynska; Bethany J. Weller; Maria Clarke; Carolyn Lam; Rico C. H. Man; Blaze G. O’ Shaughnessy; Melanie Clifford; Tam T. Bui; Alex F. Drake; R. Andrew Atkinson; Jenny K. W. Lam; Simon C. Pitchford; Clive P. Page; David A. Phoenix; Christian D. Lorenz; J. Mark Sutton; A. James Mason

    Antimicrobial peptides (AMPs) are a potential alternative to classical antibiotics that are yet to achieve a therapeutic breakthrough for treatment of systemic infections. The antibacterial potency of pleurocidin, an AMP from Winter Flounder, is linked to its ability to cross bacterial plasma membranes and seek intracellular targets while also causing membrane damage. Here we describe modification

    更新日期:2020-11-27
  • The PAR2 inhibitor I-287 selectively targets Gα q and Gα 12/13 signaling and has anti-inflammatory effects
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Charlotte Avet; Claudio Sturino; Sébastien Grastilleur; Christian Le Gouill; Meriem Semache; Florence Gross; Louis Gendron; Youssef Bennani; Joseph A. Mancini; Camil E. Sayegh; Michel Bouvier

    Protease-activated receptor-2 (PAR2) is involved in inflammatory responses and pain, therefore representing a promising therapeutic target for the treatment of immune-mediated inflammatory diseases. However, as for other GPCRs, PAR2 can activate multiple signaling pathways and those involved in inflammatory responses remain poorly defined. Here, we describe a new selective and potent PAR2 inhibitor

    更新日期:2020-11-27
  • Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Patricia Rico; Aleixandre Rodrigo-Navarro; Laura Sánchez Pérez; Manuel Salmeron-Sanchez

    The intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the

    更新日期:2020-11-27
  • Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Leigh A. Stoddart; Nicholas D. Kindon; Omolade Otun; Clare R. Harwood; Foteini Patera; Dmitry B. Veprintsev; Jeanette Woolard; Stephen J. Briddon; Hester A. Franks; Stephen J. Hill; Barrie Kellam

    To study the localisation of G protein-coupled receptors (GPCR) in their native cellular environment requires their visualisation through fluorescent labelling. To overcome the requirement for genetic modification of the receptor or the limitations of dissociable fluorescent ligands, here we describe rational design of a compound that covalently and selectively labels a GPCR in living cells with a

    更新日期:2020-11-27
  • Increased TRPV4 expression in non-myelinating Schwann cells is associated with demyelination after sciatic nerve injury
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-27
    Xiaona Feng; Yasunori Takayama; Nobuhiko Ohno; Hirosato Kanda; Yi Dai; Takaaki Sokabe; Makoto Tominaga

    Transient receptor potential vanilloid 4 (TRPV4) is a non-selective calcium-permeable cation channel that is widely expressed and activated in various neurons and glial cells in the nervous system. Schwann cells (SCs) are primary glia cells that wrap around axons to form the myelin sheath in the peripheral nervous system. However, whether TRPV4 is expressed and functions in SCs is unclear. Here, we

    更新日期:2020-11-27
  • Enhancing calmodulin binding to cardiac ryanodine receptor completely inhibits pressure-overload induced hypertrophic signaling
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-26
    Michiaki Kohno; Shigeki Kobayashi; Takeshi Yamamoto; Ryosuke Yoshitomi; Toshiro Kajii; Shohei Fujii; Yoshihide Nakamura; Takayoshi Kato; Hitoshi Uchinoumi; Tetsuro Oda; Shinichi Okuda; Kenji Watanabe; Yoichi Mizukami; Masafumi Yano

    Cardiac hypertrophy is a well-known major risk factor for poor prognosis in patients with cardiovascular diseases. Dysregulation of intracellular Ca2+ is involved in the pathogenesis of cardiac hypertrophy. However, the precise mechanism underlying cardiac hypertrophy remains elusive. Here, we investigate whether pressure-overload induced hypertrophy can be induced by destabilization of cardiac ryanodine

    更新日期:2020-11-27
  • Predicting clinical resistance prevalence using sewage metagenomic data
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-26
    Antti Karkman; Fanny Berglund; Carl-Fredrik Flach; Erik Kristiansson; D. G. Joakim Larsson

    Antibiotic resistance surveillance through regional and up-to-date testing of clinical isolates is a foundation for implementing effective empirical treatment. Surveillance data also provides an overview of geographical and temporal changes that are invaluable for guiding interventions. Still, due to limited infrastructure and resources, clinical surveillance data is lacking in many parts of the world

    更新日期:2020-11-27
  • PKN1 promotes synapse maturation by inhibiting mGluR-dependent silencing through neuronal glutamate transporter activation
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-26
    Hiroki Yasuda; Hikaru Yamamoto; Kenji Hanamura; Mona Mehruba; Toshio Kawamata; Hiromi Morisaki; Masaaki Miyamoto; Shinji Takada; Tomoaki Shirao; Yoshitaka Ono; Hideyuki Mukai

    Abnormal metabotropic glutamate receptor (mGluR) activity could cause brain disorders; however, its regulation has not yet been fully understood. Here, we report that protein kinase N1 (PKN1), a protein kinase expressed predominantly in neurons in the brain, normalizes group 1 mGluR function by upregulating a neuronal glutamate transporter, excitatory amino acid transporter 3 (EAAT3), and supports

    更新日期:2020-11-27
  • Cerebellar and hepatic alterations in ACBD5-deficient mice are associated with unexpected, distinct alterations in cellular lipid homeostasis
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-26
    Warda Darwisch; Marino von Spangenberg; Jana Lehmann; Öznur Singin; Geralt Deubert; Sandra Kühl; Johannes Roos; Heinz Horstmann; Christoph Körber; Simone Hoppe; Hongwei Zheng; Thomas Kuner; Mia L. Pras-Raves; Antoine H. C. van Kampen; Hans R. Waterham; Kathrin V. Schwarz; Jürgen G. Okun; Christian Schultz; Frédéric M. Vaz; Markus Islinger

    ACBD5 deficiency is a novel peroxisome disorder with a largely uncharacterized pathology. ACBD5 was recently identified in a tethering complex mediating membrane contacts between peroxisomes and the endoplasmic reticulum (ER). An ACBD5-deficient mouse was analyzed to correlate ACBD5 tethering functions with the disease phenotype. ACBD5-deficient mice exhibit elevated very long-chain fatty acid levels

    更新日期:2020-11-27
  • A haplotype-led approach to increase the precision of wheat breeding
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-25
    Jemima Brinton; Ricardo H. Ramirez-Gonzalez; James Simmonds; Luzie Wingen; Simon Orford; Simon Griffiths; Georg Haberer; Manuel Spannagl; Sean Walkowiak; Curtis Pozniak; Cristobal Uauy

    Crop productivity must increase at unprecedented rates to meet the needs of the growing worldwide population. Exploiting natural variation for the genetic improvement of crops plays a central role in increasing productivity. Although current genomic technologies can be used for high-throughput identification of genetic variation, methods for efficiently exploiting this genetic potential in a targeted

    更新日期:2020-11-25
  • Large genome-wide association study identifies three novel risk variants for restless legs syndrome
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-25
    Maria Didriksen; Muhammad Sulaman Nawaz; Joseph Dowsett; Steven Bell; Christian Erikstrup; Ole B. Pedersen; Erik Sørensen; Poul J. Jennum; Kristoffer S. Burgdorf; Brendan Burchell; Adam S. Butterworth; Nicole Soranzo; David B. Rye; Lynn Marie Trotti; Prabhjyot Saini; Lilja Stefansdottir; Sigurdur H. Magnusson; Gudmar Thorleifsson; Thordur Sigmundsson; Albert P. Sigurdsson; Katja Van Den Hurk; Franke

    Restless legs syndrome (RLS) is a common neurological sensorimotor disorder often described as an unpleasant sensation associated with an urge to move the legs. Here we report findings from a meta-analysis of genome-wide association studies of RLS including 480,982 Caucasians (cases = 10,257) and a follow up sample of 24,977 (cases = 6,651). We confirm 19 of the 20 previously reported RLS sequence

    更新日期:2020-11-25
  • Persistent biotic interactions of a Gondwanan conifer from Cretaceous Patagonia to modern Malesia
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-25
    Michael P. Donovan; Peter Wilf; Ari Iglesias; N. Rubén Cúneo; Conrad C. Labandeira

    Many plant genera in the tropical West Pacific are survivors from the paleo-rainforests of Gondwana. For example, the oldest fossils of the Malesian and Australasian conifer Agathis (Araucariaceae) come from the early Paleocene and possibly latest Cretaceous of Patagonia, Argentina (West Gondwana). However, it is unknown whether dependent ecological guilds or lineages of associated insects and fungi

    更新日期:2020-11-25
  • Genomic retargeting of p53 and CTCF is associated with transcriptional changes during oncogenic HRas-induced transformation
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-25
    Michal Schwartz; Avital Sarusi Portugez; Bracha Zukerman Attia; Miriam Tannenbaum; Leslie Cohen; Olga Loza; Emily Chase; Yousef Turman; Tommy Kaplan; Zaidoun Salah; Ofir Hakim

    Gene transcription is regulated by distant regulatory elements via combinatorial binding of transcription factors. It is increasingly recognized that alterations in chromatin state and transcription factor binding in these distant regulatory elements may have key roles in cancer development. Here we focused on the first stages of oncogene-induced carcinogenic transformation, and characterized the regulatory

    更新日期:2020-11-25
  • Differences in visually induced MEG oscillations reflect differences in deep cortical layer activity
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-25
    Dimitris A. Pinotsis; Earl K. Miller

    Neural activity is organized at multiple scales, ranging from the cellular to the whole brain level. Connecting neural dynamics at different scales is important for understanding brain pathology. Neurological diseases and disorders arise from interactions between factors that are expressed in multiple scales. Here, we suggest a new way to link microscopic and macroscopic dynamics through combinations

    更新日期:2020-11-25
  • Photosensitive tyrosine analogues unravel site-dependent phosphorylation in TrkA initiated MAPK/ERK signaling
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-25
    Shu Zhao; Jia Shi; Guohua Yu; Dali Li; Meng Wang; Chonggang Yuan; Huihui Zhou; Amirabbas Parizadeh; Zhenlin Li; Min-Xin Guan; Shixin Ye

    Tyrosine kinase A (TrkA) is a membrane receptor which, upon ligand binding, activates several pathways including MAPK/ERK signaling, implicated in a spectrum of human pathologies; thus, TrkA is an emerging therapeutic target in treatment of neuronal diseases and cancer. However, mechanistic insights into TrKA signaling are lacking due to lack of site-dependent phosphorylation control. Here we engineer

    更新日期:2020-11-25
  • Turnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-23
    Nicolas Pitzalis; Khalid Amari; Stéfanie Graindorge; David Pflieger; Livia Donaire; Michael Wassenegger; César Llave; Manfred Heinlein

    Virus-induced plant diseases in cultivated plants cause important damages in yield. Although the mechanisms of virus infection are intensely studied at the cell biology level, only little is known about the molecular dialog between the invading virus and the host genome. Here we describe a combinatorial genome-wide approach to identify networks of sRNAs-guided post-transcriptional regulation within

    更新日期:2020-11-23
  • Human TRPC5 structures reveal interaction of a xanthine-based TRPC1/4/5 inhibitor with a conserved lipid binding site
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-23
    David J. Wright; Katie J. Simmons; Rachel M. Johnson; David J. Beech; Stephen P. Muench; Robin S. Bon

    TRPC1/4/5 channels are non-specific cation channels implicated in a wide variety of diseases, and TRPC1/4/5 inhibitors have recently entered clinical trials. However, fundamental and translational studies require a better understanding of TRPC1/4/5 channel regulation by endogenous and exogenous factors. Although several potent and selective TRPC1/4/5 modulators have been reported, the paucity of mechanistic

    更新日期:2020-11-23
  • Targeted intracellular degradation of SARS-CoV-2 via computationally optimized peptide fusions
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-23
    Pranam Chatterjee; Manvitha Ponnapati; Christian Kramme; Alexandru M. Plesa; George M. Church; Joseph M. Jacobson

    The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has elicited a global health crisis of catastrophic proportions. With only a few vaccines approved for early or limited use, there is a critical need for effective antiviral strategies. In this study, we report a unique antiviral platform, through computational design of ACE2-derived peptides which both target the viral spike protein

    更新日期:2020-11-23
  • Pac-Man like DNA cut-and-paste tool allows larger gene edits
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-20
    Anam Akhtar

    The CRISPR-Cas toolbox allows genetic manipulation of cultured cells, plants and animals on the basis of simpler RNA-guided DNA recognition. It has provided breakthrough scientific opportunities to engineer desirable traits, cure genetic diseases and enable point-of-care diagnostics. A recent study by Joseph Bondy-Denomy and colleagues further equips this toolbox to cut larger chunks of DNA from a

    更新日期:2020-11-21
  • Human Aurora kinase inhibitor Hesperadin reveals epistatic interaction between Plasmodium falciparum PfArk1 and PfNek1 kinases
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-20
    Belinda J. Morahan; Clarissa Abrie; Keith Al-Hasani; Mitchell B. Batty; Victoria Corey; Anne N. Cowell; Jandeli Niemand; Elizabeth A. Winzeler; Lyn-Marie Birkholtz; Christian Doerig; Jose F. Garcia-Bustos

    Mitosis has been validated by numerous anti-cancer drugs as being a druggable process, and selective inhibition of parasite proliferation provides an obvious opportunity for therapeutic intervention against malaria. Mitosis is controlled through the interplay between several protein kinases and phosphatases. We show here that inhibitors of human mitotic kinases belonging to the Aurora family inhibit

    更新日期:2020-11-21
  • Bacterial second messenger 3′,5′-cyclic diguanylate attracts Caenorhabditis elegans and suppresses its immunity
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-20
    Joseph Angeloni; Yuqing Dong; Zeneng Wang; Min Cao

    Cyclic di-nucleotides are important secondary signaling molecules in bacteria that regulate a wide range of processes. In this study, we found that Caenorhabditis elegans can detect and are attracted to multiple signal molecules produced by Vibrio cholerae, specifically the 3′,5′-cyclic diguanylate (c-di-GMP), even though this bacterium kills the host at a high rate. C-di-GMP is sensed through C. elegans

    更新日期:2020-11-21
  • Glial granules contain germline proteins in the Drosophila brain, which regulate brain transcriptome
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-20
    Samuel J. Tindell; Eric C. Rouchka; Alexey L. Arkov

    Membraneless RNA-protein granules play important roles in many different cell types and organisms. In particular, granules found in germ cells have been used as a paradigm to study large and dynamic granules. These germ granules contain RNA and proteins required for germline development. Here, we unexpectedly identify large granules in specific subtypes of glial cells (“glial granules”) of the adult

    更新日期:2020-11-21
  • New insights in the coordinated amidase and glucosaminidase activity of the major autolysin (Atl) in Staphylococcus aureus
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-20
    Mulugeta Nega; Paula Maria Tribelli; Katharina Hipp; Mark Stahl; Friedrich Götz

    After bacterial cell division, the daughter cells are still covalently interlinked by the peptidoglycan network which is resolved by specific hydrolases (autolysins) to release the daughter cells. In staphylococci, the major autolysin (Atl) with its two domain enzymes, N-acetylmuramyl-L-alanine amidase (AmiA) and β-N-acetylglucosaminidase (GlcA), resolves the peptidoglycan to release the daughter cells

    更新日期:2020-11-21
  • Fairy circles in Namibia are assembled from genetically distinct grasses
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-20
    Christian Kappel; Nicola Illing; Cuong Nguyen Huu; Nichole N. Barger; Michael D. Cramer; Michael Lenhard; Jeremy J. Midgley

    Fairy circles are striking regularly sized and spaced, bare circles surrounded by Stipagrostis grasses that occur over thousands of square kilometres in Namibia. The mechanisms explaining their origin, shape, persistence and regularity remain controversial. One hypothesis for the formation of vegetation rings is based on the centrifugal expansion of a single individual grass plant, via clonal growth

    更新日期:2020-11-21
  • Sleep-wake cycles are disrupted by diseases that result in cytoplasmic crowding
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Karli Montague-Cardoso

    It doesn’t take much to disrupt our sleep. Whilst we are aware of environmental factors that can disturb our circadian rhythms, the precise mechanisms that control molecular time cues have remained elusive. Beesley and co-workers demonstrate that diseases associated with cytoplasmic crowding affect the sleep-wake cycle. They also pinpoint a precise time-limiting step in the trafficking of the pacemaker

    更新日期:2020-11-19
  • Structural basis of superinfection exclusion by bacteriophage T4 Spackle
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Ke Shi; Justin T. Oakland; Fredy Kurniawan; Nicholas H. Moeller; Surajit Banerjee; Hideki Aihara

    A bacterial cell infected with T4 phage rapidly establishes resistance against further infections by the same or closely related T-even-type bacteriophages – a phenomenon called superinfection exclusion. Here we show that one of the T4 early gene products and a periplasmic protein, Spackle, forms a stoichiometric complex with the lysozyme domain of T4 tail spike protein gp5 and potently inhibits its

    更新日期:2020-11-19
  • Disrupting Plasmodium UIS3–host LC3 interaction with a small molecule causes parasite elimination from host cells
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Sonali Setua; Francisco J. Enguita; Ângelo Ferreira Chora; Harish Ranga-prasad; Aparajita Lahree; Sofia Marques; Varadharajan Sundaramurthy; Maria M. Mota

    The malaria parasite Plasmodium obligatorily infects and replicates inside hepatocytes surrounded by a parasitophorous vacuole membrane (PVM), which is decorated by the host-cell derived autophagy protein LC3. We have previously shown that the parasite-derived, PVM-resident protein UIS3 sequesters LC3 to avoid parasite elimination by autophagy from hepatocytes. Here we show that a small molecule capable

    更新日期:2020-11-19
  • Inducible cell-specific mouse models for paired epigenetic and transcriptomic studies of microglia and astroglia
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Ana J. Chucair-Elliott; Sarah R. Ocañas; David R. Stanford; Victor A. Ansere; Kyla B. Buettner; Hunter Porter; Nicole L. Eliason; Justin J. Reid; Amanda L. Sharpe; Michael B. Stout; Michael J. Beckstead; Benjamin F. Miller; Arlan Richardson; Willard M. Freeman

    Epigenetic regulation of gene expression occurs in a cell type-specific manner. Current cell-type specific neuroepigenetic studies rely on cell sorting methods that can alter cell phenotype and introduce potential confounds. Here we demonstrate and validate a Nuclear Tagging and Translating Ribosome Affinity Purification (NuTRAP) approach for temporally controlled labeling and isolation of ribosomes

    更新日期:2020-11-19
  • Parallel fast and slow recurrent cortical processing mediates target and distractor selection in visual search
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Sarah E. Donohue; Mircea A. Schoenfeld; Jens-Max Hopf

    Visual search has been commonly used to study the neural correlates of attentional allocation in space. Recent electrophysiological research has disentangled distractor processing from target processing, showing that these mechanisms appear to operate in parallel and show electric fields of opposite polarity. Nevertheless, the localization and exact nature of this activity is unknown. Here, using MEG

    更新日期:2020-11-19
  • Neutrophil serine protease 4 is required for mast cell-dependent vascular leakage
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Andrew P. AhYoung; Sterling C. Eckard; Alvin Gogineni; Hongkang Xi; S. Jack Lin; Stefan Gerhardy; Christian Cox; Qui T. Phung; Jason A. Hackney; Anand Kumar Katakam; Mike Reichelt; Patrick Caplazi; Paolo Manzanillo; Juan Zhang; Merone Roose-Girma; Lucinda W. Tam; Robert J. Newman; Aditya Murthy; Robby M. Weimer; Jennie R. Lill; Wyne P. Lee; Michele Grimbaldeston; Daniel Kirchhofer; Menno van Lookeren

    Vascular leakage, or edema, is a serious complication of acute allergic reactions. Vascular leakage is triggered by the release of histamine and serotonin from granules within tissue-resident mast cells. Here, we show that expression of Neutrophil Serine Protease 4 (NSP4) during the early stages of mast cell development regulates mast cell-mediated vascular leakage. In myeloid precursors, the granulocyte–macrophage

    更新日期:2020-11-19
  • Otolith chemoscape analysis in whiting links fishing grounds to nursery areas
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Neil M. Burns; Charlotte R. Hopkins; David M. Bailey; Peter J. Wright

    Understanding life stage connectivity is essential to define appropriate spatial scales for fisheries management and develop effective strategies to reduce undersized bycatch. Despite many studies of population structure and connectivity in marine fish, most management units do not reflect biological populations and protection is rarely given to juvenile sources of the fished stock. Direct, quantitative

    更新日期:2020-11-19
  • Early detection of doxorubicin-induced cardiotoxicity in rats by its cardiac metabolic signature assessed with hyperpolarized MRI
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Kerstin N. Timm; Charith Perera; Vicky Ball; John A. Henry; Jack J. Miller; Matthew Kerr; James A. West; Eshita Sharma; John Broxholme; Angela Logan; Dragana Savic; Michael S. Dodd; Julian L. Griffin; Michael P. Murphy; Lisa C. Heather; Damian J. Tyler

    Doxorubicin (DOX) is a widely used chemotherapeutic agent that can cause serious cardiotoxic side effects culminating in congestive heart failure (HF). There are currently no clinical imaging techniques or biomarkers available to detect DOX-cardiotoxicity before functional decline. Mitochondrial dysfunction is thought to be a key factor driving functional decline, though real-time metabolic fluxes

    更新日期:2020-11-19
  • Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-19
    Stephanie L. Neville; Bart A. Eijkelkamp; Amber Lothian; James C. Paton; Blaine R. Roberts; Jason W. Rosch; Christopher A. McDevitt

    Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulation of metal ion homeostasis remain to be elucidated. Here, we used cadmium, a non-physiological metal ion, to investigate how the bacterial pathogen, Streptococcus pneumoniae, resists metal ion stress and dyshomeostasis. By combining transcriptomics, metabolomics and metalloproteomics

    更新日期:2020-11-19
  • Autonomous adaptive data acquisition for scanning hyperspectral imaging
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-18
    Elizabeth A. Holman; Yuan-Sheng Fang; Liang Chen; Michael DeWeese; Hoi-Ying N. Holman; Paul W. Sternberg

    Non-invasive and label-free spectral microscopy (spectromicroscopy) techniques can provide quantitative biochemical information complementary to genomic sequencing, transcriptomic profiling, and proteomic analyses. However, spectromicroscopy techniques generate high-dimensional data; acquisition of a single spectral image can range from tens of minutes to hours, depending on the desired spatial resolution

    更新日期:2020-11-18
  • Spatial organization in microbial range expansion emerges from trophic dependencies and successful lineages
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-18
    Benedict Borer; Davide Ciccarese; David Johnson; Dani Or

    Evidence suggests that bacterial community spatial organization affects their ecological function, yet details of the mechanisms that promote spatial patterns remain difficult to resolve experimentally. In contrast to bacterial communities in liquid cultures, surface-attached range expansion fosters genetic segregation of the growing population with preferential access to nutrients and reduced mechanical

    更新日期:2020-11-18
  • Genome-wide association studies and heritability analysis reveal the involvement of host genetics in the Japanese gut microbiota
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-18
    Sachiko Ishida; Kumiko Kato; Masami Tanaka; Toshitaka Odamaki; Ryuichi Kubo; Eri Mitsuyama; Jin-zhong Xiao; Rui Yamaguchi; Satoshi Uematsu; Seiya Imoto; Satoru Miyano

    Numerous host extrinsic and intrinsic factors affect the gut microbiota composition, but their cumulative effects do not sufficiently explain the variation in the microbiota, suggesting contributions of missing factors. The Japanese population possesses homogeneous genetic features suitable for genome-wide association study (GWAS). Here, we performed GWASs for human gut microbiota using 1068 healthy

    更新日期:2020-11-18
  • Methamphetamine induces cardiomyopathy by Sigmar1 inhibition-dependent impairment of mitochondrial dynamics and function
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-17
    Chowdhury S. Abdullah; Richa Aishwarya; Shafiul Alam; Mahboob Morshed; Naznin Sultana Remex; Sadia Nitu; Gopi K. Kolluru; James Traylor; Sumitra Miriyala; Manikandan Panchatcharam; Brandon Hartman; Judy King; Mohammad Alfrad Nobel Bhuiyan; Sunitha Chandran; Matthew D. Woolard; Xiuping Yu; Nicholas E. Goeders; Paari Dominic; Connie L. Arnold; Karen Stokes; Christopher G. Kevil; A. Wayne Orr; Md. Shenuarin

    Methamphetamine-associated cardiomyopathy is the leading cause of death linked with illicit drug use. Here we show that Sigmar1 is a therapeutic target for methamphetamine-associated cardiomyopathy and defined the molecular mechanisms using autopsy samples of human hearts, and a mouse model of “binge and crash” methamphetamine administration. Sigmar1 expression is significantly decreased in the hearts

    更新日期:2020-11-17
  • A symmoriiform from the Late Devonian of Morocco demonstrates a derived jaw function in ancient chondrichthyans
    Commun. Biol. (IF 4.165) Pub Date : 2020-11-17
    Linda Frey; Michael I. Coates; Kristen Tietjen; Martin Rücklin; Christian Klug

    The Palaeozoic record of chondrichthyans (sharks, rays, chimaeras, extinct relatives) and thus our knowledge of their anatomy and functional morphology is poor because of their predominantly cartilaginous skeletons. Here, we report a previously undescribed symmoriiform shark, Ferromirum oukherbouchi, from the Late Devonian of the Anti-Atlas. Computed tomography scanning reveals the undeformed shape

    更新日期:2020-11-17