当前期刊: Communications Biology Go to current issue    加入关注   
显示样式:        排序: IF: - GO 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Transcriptome-wide association study for restless legs syndrome identifies new susceptibility genes
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-10
    Fulya Akçimen; Faezeh Sarayloo; Calwing Liao; Jay P. Ross; Rachel De Barros Oliveira; Patrick A. Dion; Guy A. Rouleau

    Restless legs syndrome (RLS) is a common neurological condition, with a prevalence of 5–15% in Central Europe and North America. Although genome-wide association studies (GWAS) have identified some common risk regions for RLS, the causal genes have yet to be fully elucidated. We conducted a transcriptome-wide association study involving 15,126 RLS cases and 95,725 controls, from the most recent meta-analysis

    更新日期:2020-07-10
  • Molecular profiling of lipid droplets inside HuH7 cells with Raman micro-spectroscopy
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-10
    Ashok Zachariah Samuel; Rimi Miyaoka; Masahiro Ando; Anne Gaebler; Christoph Thiele; Haruko Takeyama

    Raman imaging has become an attractive technology in molecular biology because of its ability to detect multiple molecular components simultaneously without labeling. Two major limitations in accurately accounting for spectral features, viz., background removal and spectral unmixing, have been overcome by employing a modified and effective routine in multivariate curve resolution (MCR). With our improved

    更新日期:2020-07-10
  • Tract-specific analysis improves sensitivity of spinal cord diffusion MRI to cross-sectional and longitudinal changes in amyotrophic lateral sclerosis
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-10
    Pramod Kumar Pisharady; Lynn E. Eberly; Ian Cheong; Georgios Manousakis; Gaurav Guliani; H. Brent Clark; Mark Bathe; David Walk; Christophe Lenglet

    Amyotrophic lateral sclerosis (ALS) is a late-onset fatal neurodegenerative disease that causes progressive degeneration of motor neurons in the brain and the spinal cord. Corticospinal tract degeneration is a defining feature of ALS. However, there have been very few longitudinal, controlled studies assessing diffusion MRI (dMRI) metrics in different fiber tracts along the spinal cord in general or

    更新日期:2020-07-10
  • Genomic insights of body plan transitions from bilateral to pentameral symmetry in Echinoderms
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-10
    Yongxin Li; Akihito Omori; Rachel L. Flores; Sheri Satterfield; Christine Nguyen; Tatsuya Ota; Toko Tsurugaya; Tetsuro Ikuta; Kazuho Ikeo; Mani Kikuchi; Jason C. K. Leong; Adrian Reich; Meng Hao; Wenting Wan; Yang Dong; Yaondong Ren; Si Zhang; Tao Zeng; Masahiro Uesaka; Yui Uchida; Xueyan Li; Tomoko F. Shibata; Takahiro Bino; Kota Ogawa; Shuji Shigenobu; Mariko Kondo; Fayou Wang; Luonan Chen; Gary

    Echinoderms are an exceptional group of bilaterians that develop pentameral adult symmetry from a bilaterally symmetric larva. However, the genetic basis in evolution and development of this unique transformation remains to be clarified. Here we report newly sequenced genomes, developmental transcriptomes, and proteomes of diverse echinoderms including the green sea urchin (L. variegatus), a sea cucumber

    更新日期:2020-07-10
  • Dynamic palmitoylation events following T-cell receptor signaling
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-10
    Eliot Morrison; Tatjana Wegner; Andres Ernesto Zucchetti; Miguel Álvaro-Benito; Ashley Zheng; Stefanie Kliche; Eberhard Krause; Britta Brügger; Claire Hivroz; Christian Freund

    Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. The reversible nature of this modification makes it a prime candidate as a mechanism for regulating signal transduction in T-cell receptor signaling. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras

    更新日期:2020-07-10
  • A smart polymer for sequence-selective binding, pulldown, and release of DNA targets
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-10
    Elisha Krieg; Krishna Gupta; Andreas Dahl; Mathias Lesche; Susanne Boye; Albena Lederer; William M. Shih

    Selective isolation of DNA is crucial for applications in biology, bionanotechnology, clinical diagnostics and forensics. We herein report a smart methanol-responsive polymer (MeRPy) that can be programmed to bind and separate single- as well as double-stranded DNA targets. Captured targets are quickly isolated and released back into solution by denaturation (sequence-agnostic) or toehold-mediated

    更新日期:2020-07-10
  • Mandarin fish (Sinipercidae) genomes provide insights into innate predatory feeding
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Shan He; Ling Li; Li-Yuan Lv; Wen-Jing Cai; Ya-Qi Dou; Jiao Li; Shu-Lin Tang; Xu Chen; Zhen Zhang; Jing Xu; Yan-Peng Zhang; Zhan Yin; Sven Wuertz; Ya-Xiong Tao; Heiner Kuhl; Xu-Fang Liang

    Mandarin fishes (Sinipercidae) are piscivores that feed solely on live fry. Unlike higher vertebrates, teleosts exhibit feeding behavior driven mainly by genetic responses, with no modification by learning from parents. Mandarin fishes could serve as excellent model organisms for studying feeding behavior. We report a long-read, chromosomal-scale genome assembly for Siniperca chuatsi and genome assemblies

    更新日期:2020-07-09
  • STK11 (LKB1) missense somatic mutant isoforms promote tumor growth, motility and inflammation
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Paula Granado-Martínez; Sara Garcia-Ortega; Elena González-Sánchez; Kimberley McGrail; Rafael Selgas; Judit Grueso; Rosa Gil; Neia Naldaiz-Gastesi; Ana C. Rhodes; Javier Hernandez-Losa; Berta Ferrer; Francesc Canals; Josep Villanueva; Olga Méndez; Sergio Espinosa-Gil; José M. Lizcano; Eva Muñoz-Couselo; Vicenç García-Patos; Juan A. Recio

    Elucidating the contribution of somatic mutations to cancer is essential for personalized medicine. STK11 (LKB1) appears to be inactivated in human cancer. However, somatic missense mutations also occur, and the role/s of these alterations to this disease remain unknown. Here, we investigated the contribution of four missense LKB1 somatic mutations in tumor biology. Three out of the four mutants lost

    更新日期:2020-07-09
  • Clustering of human prion protein and α-synuclein oligomers requires the prion protein N-terminus
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Nadine S. Rösener; Lothar Gremer; Michael M. Wördehoff; Tatsiana Kupreichyk; Manuel Etzkorn; Philipp Neudecker; Wolfgang Hoyer

    The interaction of prion protein (PrP) and α-synuclein (αSyn) oligomers causes synaptic impairment that might trigger Parkinson’s disease and other synucleinopathies. Here, we report that αSyn oligomers (αSynO) cluster with human PrP (huPrP) into micron-sized condensates. Multivalency of αSyn within oligomers is required for condensation, since clustering with huPrP is not observed for monomeric αSyn

    更新日期:2020-07-09
  • Reciprocal H3.3 gene editing identifies K27M and G34R mechanisms in pediatric glioma including NOTCH signaling
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Kuang-Yui Chen; Kelly Bush; Rachel Herndon Klein; Vanessa Cervantes; Nichole Lewis; Aasim Naqvi; Angel M. Carcaboso; Mirna Lechpammer; Paul S. Knoepfler

    Histone H3.3 mutations are a hallmark of pediatric gliomas, but their core oncogenic mechanisms are not well-defined. To identify major effectors, we used CRISPR-Cas9 to introduce H3.3K27M and G34R mutations into previously H3.3-wildtype brain cells, while in parallel reverting the mutations in glioma cells back to wildtype. ChIP-seq analysis broadly linked K27M to altered H3K27me3 activity including

    更新日期:2020-07-09
  • Correlation of fluorescence microscopy, electron microscopy, and NanoSIMS stable isotope imaging on a single tissue section
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Céline Loussert-Fonta; Gaëlle Toullec; Arun Aby Paraecattil; Quentin Jeangros; Thomas Krueger; Stephane Escrig; Anders Meibom

    Correlative light and electron microscopy allows localization of specific molecules at the ultrastructural level in biological tissue but does not provide information about metabolic turnover or the distribution of labile molecules, such as micronutrients. We present a method to directly correlate (immuno)fluorescent microscopy, (immuno)TEM imaging and NanoSIMS isotopic mapping of the same tissue section

    更新日期:2020-07-09
  • Sequence-based genetic mapping of Cynodon dactylon Pers. reveals new insights into genome evolution in Poaceae
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Tilin Fang; Hongxu Dong; Shuhao Yu; Justin Q. Moss; Charles H. Fontanier; Dennis L. Martin; Jinmin Fu; Yanqi Wu

    Bermudagrass (Cynodon dactylon Pers.) is an important warm-season perennial used extensively for turf, forage, soil conservation and remediation worldwide. However, limited genomic information has hindered the application of molecular tools towards understanding genome evolution and in breeding new cultivars. We genotype a first-generation selfed population derived from the tetraploid (4x = 36) ‘A12359’

    更新日期:2020-07-09
  • Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson’s disease
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Laurence Lemelle; Alexandre Simionovici; Philippe Colin; Graham Knott; Sylvain Bohic; Peter Cloetens; Bernard L. Schneider

    Sub-cellular trace element quantifications of nano-heterogeneities in brain tissues offer unprecedented ways to explore at elemental level the interplay between cellular compartments in neurodegenerative pathologies. We designed a quasi-correlative method for analytical nanoimaging of the substantia nigra, based on transmission electron microscopy and synchrotron X-ray fluorescence. It combines ultrastructural

    更新日期:2020-07-09
  • Perceptual metacognition of human faces is causally supported by function of the lateral prefrontal cortex
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Regina C. Lapate; Jason Samaha; Bas Rokers; Bradley R. Postle; Richard J. Davidson

    Metacognitive awareness—the ability to know that one is having a particular experience—is thought to guide optimal behavior, but its neural bases continue to be the subject of vigorous debate. Prior work has identified correlations between perceptual metacognitive ability and the structure and function of lateral prefrontal cortex (LPFC); however, evidence for a causal role of this region in promoting

    更新日期:2020-07-09
  • Evaluation of molecular subtypes and clonal selection during establishment of patient-derived tumor xenografts from gastric adenocarcinoma
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Anne-Lise Peille; Vincent Vuaroqueaux; Swee-Seong Wong; Jason Ting; Kerstin Klinger; Bruno Zeitouni; Manuel Landesfeind; Woo Ho Kim; Hyuk-Joon Lee; Seong-Ho Kong; Isabella Wulur; Steven Bray; Peter Bronsert; Nina Zanella; Greg Donoho; Han-Kwang Yang; Heinz-Herbert Fiebig; Christoph Reinhard; Amit Aggarwal

    Patient-derived xenografts (PDX) have emerged as an important translational research tool for understanding tumor biology and enabling drug efficacy testing. They are established by transfer of patient tumor into immune compromised mice with the intent of using them as Avatars; operating under the assumption that they closely resemble patient tumors. In this study, we established 27 PDX from 100 resected

    更新日期:2020-07-09
  • Combining host-derived biomarkers with patient characteristics improves signature performance in predicting tuberculosis treatment outcomes
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-09
    Dhanasekaran Sivakumaran; Synne Jenum; Mario Vaz; Sumithra Selvam; Tom H. M. Ottenhoff; Marielle C. Haks; Stephanus T. Malherbe; T. Mark Doherty; Christian Ritz; Harleen M. S. Grewal

    Tuberculosis (TB) is a global health concern. Treatment is prolonged, and patients on anti-TB therapy (ATT) often experience treatment failure for various reasons. There is an urgent need to identify signatures for early detection of failure and initiation of a treatment switch. We investigated how gene biomarkers and/or basic patient characteristics could be used to define signatures for treatment

    更新日期:2020-07-09
  • Absence of central tolerance in Aire-deficient mice synergizes with immune-checkpoint inhibition to enhance antitumor responses
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-08
    Asiel A. Benitez; Sara Khalil-Agüero; Anjali Nandakumar; Namita T. Gupta; Wen Zhang; Gurinder S. Atwal; Andrew J. Murphy; Matthew A. Sleeman; Sokol Haxhinasto

    The endogenous anti-tumor responses are limited in part by the absence of tumor-reactive T cells, an inevitable consequence of thymic central tolerance mechanisms ensuring prevention of autoimmunity. Here we show that tumor rejection induced by immune checkpoint blockade is significantly enhanced in Aire-deficient mice, the epitome of central tolerance breakdown. The observed synergy in tumor rejection

    更新日期:2020-07-08
  • The ubiquitous flavonoid quercetin is an atypical KCNQ potassium channel activator
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-08
    Kaitlyn E. Redford; Geoffrey W. Abbott

    Many commonly consumed plants are used as folk medicines, often with unclear molecular mechanisms. Recent studies uncovered the ubiquitous and influential KCNQ family of voltage-gated potassium (Kv) channels as a therapeutic target for several medicinal plant compounds. Capers - immature flower buds of Capparis spinosa - have been consumed for food and medicinal purposes for millennia. Here, we show

    更新日期:2020-07-08
  • A marine photosynthetic microbial cell factory as a platform for spider silk production
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-08
    Choon Pin Foong; Mieko Higuchi-Takeuchi; Ali D. Malay; Nur Alia Oktaviani; Chonprakun Thagun; Keiji Numata

    Photosynthetic microorganisms such as cyanobacteria, purple bacteria and microalgae have attracted great interest as promising platforms for economical and sustainable production of bioenergy, biochemicals, and biopolymers. Here, we demonstrate heterotrophic production of spider dragline silk proteins, major ampullate spidroins (MaSp), in a marine photosynthetic purple bacterium, Rhodovulum sulfidophilum

    更新日期:2020-07-08
  • Sex differences in SARS-CoV-2 infection rates and the potential link to prostate cancer
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-08
    Dimple Chakravarty; Sujit S. Nair; Nada Hammouda; Parita Ratnani; Yasmine Gharib; Vinayak Wagaskar; Nihal Mohamed; Dara Lundon; Zachary Dovey; Natasha Kyprianou; Ashutosh K. Tewari

    The recent outbreak of infections and the pandemic caused by SARS-CoV-2 represent one of the most severe threats to human health in more than a century. Emerging data from the United States and elsewhere suggest that the disease is more severe in men. Knowledge gained, and lessons learned, from studies of the biological interactions and molecular links that may explain the reasons for the greater severity

    更新日期:2020-07-08
  • A systemic approach to assess the potential and risks of wildlife culling for infectious disease control
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-07
    Eve Miguel; Vladimir Grosbois; Alexandre Caron; Diane Pople; Benjamin Roche; Christl A. Donnelly

    The maintenance of infectious diseases requires a sufficient number of susceptible hosts. Host culling is a potential control strategy for animal diseases. However, the reduction in biodiversity and increasing public concerns regarding the involved ethical issues have progressively challenged the use of wildlife culling. Here, we assess the potential of wildlife culling as an epidemiologically sound

    更新日期:2020-07-07
  • A time-dependent diffusion MRI signature of axon caliber variations and beading
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-07
    Hong-Hsi Lee; Antonios Papaioannou; Sung-Lyoung Kim; Dmitry S. Novikov; Els Fieremans

    MRI provides a unique non-invasive window into the brain, yet is limited to millimeter resolution, orders of magnitude coarser than cell dimensions. Here, we show that diffusion MRI is sensitive to the micrometer-scale variations in axon caliber or pathological beading, by identifying a signature power-law diffusion time-dependence of the along-fiber diffusion coefficient. We observe this signature

    更新日期:2020-07-07
  • β-amyloid and tau drive early Alzheimer’s disease decline while glucose hypometabolism drives late decline
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-06
    Tyler C. Hammond; Xin Xing; Chris Wang; David Ma; Kwangsik Nho; Paul K. Crane; Fanny Elahi; David A. Ziegler; Gongbo Liang; Qiang Cheng; Lucille M. Yanckello; Nathan Jacobs; Ai-Ling Lin

    Clinical trials focusing on therapeutic candidates that modify β-amyloid (Aβ) have repeatedly failed to treat Alzheimer’s disease (AD), suggesting that Aβ may not be the optimal target for treating AD. The evaluation of Aβ, tau, and neurodegenerative (A/T/N) biomarkers has been proposed for classifying AD. However, it remains unclear whether disturbances in each arm of the A/T/N framework contribute

    更新日期:2020-07-06
  • Structure of a DNA polymerase abortive complex with the 8OG:dA base pair at the primer terminus
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Vinod K. Batra; Samuel H. Wilson

    Adenine frequently pairs with the Hoogsteen edge of an oxidized guanine base (8OG) causing G to T transversions. The (syn)8OG:dA base pair is indistinguishable from the cognant base pair and can be extended by DNA polymerases with reduced efficiency. To examine the structural basis of this reduced efficiency, we sought to obtain the structure of the “product” complex of DNA polymerase (pol) β with

    更新日期:2020-07-03
  • Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Mattias Brofelth; Anna Isinger Ekstrand; Shashank Gour; Ronnie Jansson; My Hedhammar; Björn Elleby; Anders Kvist; Christer Wingren; Ulrika Axelsson; Carl A. K. Borrebaeck

    The composition of serum proteins is reflecting the current health status and can, with the right tools, be used to detect early signs of disease, such as an emerging cancer. An earlier diagnosis of cancer would greatly increase the chance of an improved outcome for the patients. However, there is still an unmet need for proficient tools to decipher the information in the blood proteome, which calls

    更新日期:2020-07-03
  • Task rule and choice are reflected by layer-specific processing in rodent auditory cortical microcircuits
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Marina M. Zempeltzi; Martin Kisse; Michael G. K. Brunk; Claudia Glemser; Sümeyra Aksit; Katrina E. Deane; Shivam Maurya; Lina Schneider; Frank W. Ohl; Matthias Deliano; Max F. K. Happel

    The primary auditory cortex (A1) is an essential, integrative node that encodes the behavioral relevance of acoustic stimuli, predictions, and auditory-guided decision-making. However, the realization of this integration with respect to the cortical microcircuitry is not well understood. Here, we characterize layer-specific, spatiotemporal synaptic population activity with chronic, laminar current

    更新日期:2020-07-03
  • Subtyping analysis reveals new variants and accelerated evolution of Clostridioides difficile toxin B
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Enhui Shen; Kangli Zhu; Danyang Li; Zhenrui Pan; Yun Luo; Qiao Bian; Liuqing He; Xiaojun Song; Ying Zhen; Dazhi Jin; Liang Tao

    Clostridioides difficile toxins (TcdA and TcdB) are major exotoxins responsible for C. difficile infection (CDI) associated diseases. The previously reported TcdB variants showed distinct biological features, immunoactivities, and potential pathogenicity in disease progression. Here, we performed global comparisons of amino acid sequences of both TcdA and TcdB from 3,269 C. difficile genomes and clustered

    更新日期:2020-07-03
  • Zeb1 promotes corneal neovascularization by regulation of vascular endothelial cell proliferation
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Lei Jin; Yingnan Zhang; Wei Liang; Xiaoqin Lu; Niloofar Piri; Wei Wang; Henry J. Kaplan; Douglas C. Dean; Lijun Zhang; Yongqing Liu

    Angiogenesis is required for tissue repair; but abnormal angiogenesis or neovascularization (NV) causes diseases in the eye. The avascular status in the cornea is a prerequisite for corneal clarity and thought to be maintained by the equilibrium between proangiogenic and antiangiogenic factors that controls proliferation and migration of vascular endothelial cells (ECs) sprouting from the pericorneal

    更新日期:2020-07-03
  • Select amino acids in DGCR8 are essential for the UGU-pri-miRNA interaction and processing
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Thi Lieu Dang; Cong Truc Le; Minh Ngoc Le; Trung Duc Nguyen; Thuy Linh Nguyen; Sheng Bao; Shaohua Li; Tuan Anh Nguyen

    Microprocessor, composed of DROSHA and DGCR8, processes primary microRNAs (pri-miRNAs) in miRNA biogenesis. Its cleavage efficiency and accuracy are enhanced because DGCR8 interacts with the apical UGU motif of pri-miRNAs. However, the mechanism and influence of DGCR8–UGU interaction on cellular miRNA expression are still elusive. In this study, we demonstrated that Rhed (i.e., the RNA-binding heme

    更新日期:2020-07-03
  • Geometric regulation of histone state directs melanoma reprogramming
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Junmin Lee; Thomas G. Molley; Christopher H. Seward; Amr A. Abdeen; Huimin Zhang; Xiaochun Wang; Hetvi Gandhi; Jia-Lin Yang; Katharina Gaus; Kristopher A. Kilian

    Malignant melanoma displays a high degree of cellular plasticity during disease progression. Signals in the tumor microenvironment are believed to influence melanoma plasticity through changes in the epigenetic state to guide dynamic differentiation and de-differentiation. Here we uncover a relationship between geometric features at perimeter regions of melanoma aggregates, and reprogramming to a stem

    更新日期:2020-07-03
  • Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Keita Hibi; Kazuaki Amikura; Naoki Sugiura; Keiko Masuda; Satoshi Ohno; Takashi Yokogawa; Takuya Ueda; Yoshihiro Shimizu

    Entire reconstitution of tRNAs for active protein production in a cell-free system brings flexibility into the genetic code engineering. It can also contribute to the field of cell-free synthetic biology, which aims to construct self-replicable artificial cells. Herein, we developed a system equipped only with in vitro transcribed tRNA (iVTtRNA) based on a reconstituted cell-free protein synthesis

    更新日期:2020-07-03
  • Rapid activation of distinct members of multigene families in Plasmodium spp
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Radoslaw Igor Omelianczyk; Han Ping Loh; Marvin Chew; Regina Hoo; Sebastian Baumgarten; Laurent Renia; Jianzhu Chen; Peter R. Preiser

    The genomes of Plasmodium spp. encode a number of different multigene families that are thought to play a critical role for survival. However, with the exception of the P. falciparum var genes, very little is known about the biological roles of any of the other multigene families. Using the recently developed Selection Linked Integration method, we have been able to activate the expression of a single

    更新日期:2020-07-03
  • Salmonella -based platform for efficient delivery of functional binding proteins to the cytosol
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Antoine Chabloz; Jonas V. Schaefer; Ivona Kozieradzki; Shane J. F. Cronin; Daniel Strebinger; Francesca Macaluso; Jiri Wald; Terence H. Rabbitts; Andreas Plückthun; Thomas C. Marlovits; Josef M. Penninger

    Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of cells remains a major challenge; thus cytosolic applications of protein-based reagents are rather limited

    更新日期:2020-07-03
  • Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Gérard Triqueneaux; Claire Burny; Orsolya Symmons; Stéphane Janczarski; Henri Gruffat; Gaël Yvert

    Variability in gene expression across a population of homogeneous cells is known to influence various biological processes. In model organisms, natural genetic variants were found that modify expression dispersion (variability at a fixed mean) but very few studies have detected such effects in humans. Here, we analyzed single-cell expression of four proteins (CD23, CD55, CD63 and CD86) across cell

    更新日期:2020-07-03
  • DNA methylation reprogramming during seed development and its functional relevance in seed size/weight determination in chickpea
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Mohan Singh Rajkumar; Khushboo Gupta; Niraj Kumar Khemka; Rohini Garg; Mukesh Jain

    Seed development is orchestrated via complex gene regulatory networks and pathways. Epigenetic factors may also govern seed development and seed size/weight. Here, we analyzed DNA methylation in a large-seeded chickpea cultivar (JGK 3) during seed development stages. Progressive gain of CHH context DNA methylation in transposable elements (TEs) and higher frequency of small RNAs in hypermethylated

    更新日期:2020-07-03
  • A Cu 9 S 5 nanoparticle-based CpG delivery system for synergistic photothermal-, photodynamic- and immunotherapy
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Lulu Zhou; Lv Chen; Xiaochun Hu; Yonglin Lu; Wenjie Liu; Yanting Sun; Tianming Yao; Chunyan Dong; Shuo Shi

    Despite its great potential in cancer therapy, phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT), often cause metastasis of tumors. Immunotherapy has revolutionized the cancer treatment owing to the capability of activating immune system to eliminate tumors. However, the integration of phototherapy and immunotherapy in a single nanoagent for cancer therapy is still a

    更新日期:2020-07-03
  • Spatio-molecular domains identified in the mouse subthalamic nucleus and neighboring glutamatergic and GABAergic brain structures
    Commun. Biol. (IF 4.165) Pub Date : 2020-07-03
    Åsa Wallén-Mackenzie; Sylvie Dumas; Maria Papathanou; Mihaela M. Martis Thiele; Bianca Vlcek; Niclas König; Åsa K. Björklund

    The subthalamic nucleus (STN) is crucial for normal motor, limbic and associative function. STN dysregulation is correlated with several brain disorders, including Parkinsonʼs disease and obsessive compulsive disorder (OCD), for which high-frequency stimulation of the STN is increasing as therapy. However, clinical progress is hampered by poor knowledge of the anatomical–functional organization of

    更新日期:2020-07-03
  • Estimating and abstracting the 3D structure of feline bones using neural networks on X-ray (2D) images
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-30
    Jana Čavojská; Julian Petrasch; Denny Mattern; Nicolas Jens Lehmann; Agnès Voisard; Peter Böttcher

    Computing 3D bone models using traditional Computed Tomography (CT) requires a high-radiation dose, cost and time. We present a fully automated, domain-agnostic method for estimating the 3D structure of a bone from a pair of 2D X-ray images. Our triplet loss-trained neural network extracts a 128-dimensional embedding of the 2D X-ray images. A classifier then finds the most closely matching 3D bone

    更新日期:2020-06-30
  • Non-invasive red-light optogenetic control of Drosophila cardiac function
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-29
    Jing Men; Airong Li; Jason Jerwick; Zilong Li; Rudolph E. Tanzi; Chao Zhou

    Drosophila is a powerful genetic model system for cardiovascular studies. Recently, optogenetic pacing tools have been developed to control Drosophila heart rhythm noninvasively with blue light, which has a limited penetration depth. Here we developed both a red-light sensitive opsin expressing Drosophila system and an integrated red-light stimulation and optical coherence microscopy (OCM) imaging

    更新日期:2020-06-29
  • Fungal mycelium classified in different material families based on glycerol treatment
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-26
    Freek V. W. Appels; Jeroen G. van den Brandhof; Jan Dijksterhuis; Gijs W. de Kort; Han A. B. Wösten

    Fungal mycelium is an emerging bio-based material. Here, mycelium films are produced from liquid shaken cultures that have a Young’s modulus of 0.47 GPa, an ultimate tensile strength of 5.0 MPa and a strain at failure of 1.5%. Treating the mycelial films with 0–32% glycerol impacts the material properties. The largest effect is observed after treatment with 32% glycerol decreasing the Young’s modulus

    更新日期:2020-06-26
  • Long-term effects of neonicotinoid insecticides on ants
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-26
    Daniel Schläppi; Nina Kettler; Lars Straub; Gaétan Glauser; Peter Neumann

    The widespread prophylactic usage of neonicotinoid insecticides has a clear impact on non-target organisms. However, the possible effects of long-term exposure on soil-dwelling organisms are still poorly understood especially for social insects with long-living queens. Here, we show that effects of chronic exposure to the neonicotinoid thiamethoxam on black garden ant colonies, Lasius niger, become

    更新日期:2020-06-26
  • Temporal structure of mouse courtship vocalizations facilitates syllable labeling
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-26
    Stav Hertz; Benjamin Weiner; Nisim Perets; Michael London

    Mice emit sequences of ultrasonic vocalizations (USVs) but little is known about the rules governing their temporal order and no consensus exists on the classification of USVs into syllables. To address these questions, we recorded USVs during male-female courtship and found a significant temporal structure. We labeled USVs using three popular algorithms and found that there was no one-to-one relationships

    更新日期:2020-06-26
  • Robust detection of oncometabolic aberrations by 1 H– 13 C heteronuclear single quantum correlation in intact biological specimens
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-25
    Yasaman Barekatain; Victoria C. Yan; Kenisha Arthur; Jeffrey J. Ackroyd; Sunada Khadka; John De Groot; Jason T. Huse; Florian L. Muller

    Magnetic resonance (MR) spectroscopy has potential to non-invasively detect metabolites of diagnostic significance for precision oncology. Yet, many metabolites have similar chemical shifts, yielding highly convoluted 1H spectra of intact biological material and limiting diagnostic utility. Here, we show that hydrogen–carbon heteronuclear single quantum correlation (1H–13C HSQC) offers dramatic improvements

    更新日期:2020-06-25
  • Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-25
    Suradip Das; Kevin D. Browne; Franco A. Laimo; Joseph C. Maggiore; Melanie C. Hilman; Halimulati Kaisaier; Carlos A. Aguilar; Zarina S. Ali; Foteini Mourkioti; D. Kacy Cullen

    Volumetric muscle loss (VML) is the traumatic or surgical loss of skeletal muscle beyond the inherent regenerative capacity of the body, generally leading to severe functional deficit. Formation of appropriate somato-motor innervations remains one of the biggest challenges for both autologous grafts as well as tissue-engineered muscle constructs. We aim to address this challenge by developing pre-innervated

    更新日期:2020-06-25
  • Single-cell sequencing of genomic DNA resolves sub-clonal heterogeneity in a melanoma cell line
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-25
    Enrique I. Velazquez-Villarreal; Shamoni Maheshwari; Jon Sorenson; Ian T. Fiddes; Vijay Kumar; Yifeng Yin; Michelle G. Webb; Claudia Catalanotti; Mira Grigorova; Paul A. Edwards; John D. Carpten; David W. Craig

    We performed shallow single-cell sequencing of genomic DNA across 1475 cells from a cell-line, COLO829, to resolve overall complexity and clonality. This melanoma tumor-line has been previously characterized by multiple technologies and is a benchmark for evaluating somatic alterations. In some of these studies, COLO829 has shown conflicting and/or indeterminate copy number and, thus, single-cell sequencing

    更新日期:2020-06-25
  • Analysis of genetically independent phenotypes identifies shared genetic factors associated with chronic musculoskeletal pain conditions
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-25
    Yakov A. Tsepilov; Maxim B. Freidin; Alexandra S. Shadrina; Sodbo Z. Sharapov; Elizaveta E. Elgaeva; Jan van Zundert; Lennart С. Karssen; Pradeep Suri; Frances M. K. Williams; Yurii S. Aulchenko

    Chronic musculoskeletal pain affects all aspects of human life. However, mechanisms of its genetic control remain poorly understood. Genetic studies of pain are complicated by the high complexity and heterogeneity of pain phenotypes. Here, we apply principal component analysis to reduce phenotype heterogeneity of chronic musculoskeletal pain at four locations: the back, neck/shoulder, hip, and knee

    更新日期:2020-06-25
  • Biocrusts buffer against the accumulation of soil metallic nutrients induced by warming and rainfall reduction
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-24
    Eduardo Moreno-Jiménez; Raúl Ochoa-Hueso; César Plaza; Sara Aceña-Heras; Maren Flagmeier; Fatima Z. Elouali; Victoria Ochoa; Beatriz Gozalo; Roberto Lázaro; Fernando T. Maestre

    The availability of metallic nutrients in dryland soils, many of which are essential for the metabolism of soil organisms and vascular plants, may be altered due to climate change-driven increases in aridity. Biocrusts, soil surface communities dominated by lichens, bryophytes and cyanobacteria, are ecosystem engineers known to exert critical functions in dryland ecosystems. However, their role in

    更新日期:2020-06-24
  • SAMHD1 is a key regulator of the lineage-specific response of acute lymphoblastic leukaemias to nelarabine
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-24
    Tamara Rothenburger; Katie-May McLaughlin; Tobias Herold; Constanze Schneider; Thomas Oellerich; Florian Rothweiler; Andrew Feber; Tim R. Fenton; Mark N. Wass; Oliver T. Keppler; Martin Michaelis; Jindrich Cinatl

    The nucleoside analogue nelarabine, the prodrug of arabinosylguanine (AraG), is effective against T-cell acute lymphoblastic leukaemia (T-ALL) but not against B-cell ALL (B-ALL). The underlying mechanisms have remained elusive. Here, data from pharmacogenomics studies and a panel of ALL cell lines reveal an inverse correlation between nelarabine sensitivity and the expression of SAMHD1, which can hydrolyse

    更新日期:2020-06-24
  • Farnesoid X receptor activation inhibits TGFBR1/TAK1-mediated vascular inflammation and calcification via miR-135a-5p
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-24
    Chao Li; Shijun Zhang; Xiaoqing Chen; Jingkang Ji; Wenqing Yang; Ting Gui; Zhibo Gai; Yunlun Li

    Chronic inflammation plays a crucial role in vascular calcification. However, only a few studies have revealed the mechanisms underlying the development of inflammation under high-phosphate conditions in chronic kidney disease (CKD) patients. Here, we show that inflammation resulting from the activation of the TGFBR1/TAK1 pathway is involved in calcification in CKD rats or osteogenic medium-cultured

    更新日期:2020-06-24
  • Vector competence of Aedes albopictus populations for chikungunya virus is shaped by their demographic history
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-24
    Anubis Vega-Rúa; Michele Marconcini; Yoann Madec; Mosè Manni; Davide Carraretto; Ludvik Marcus Gomulski; Giuliano Gasperi; Anna-Bella Failloux; Anna Rodolfa Malacrida

    The mosquito Aedes albopictus is one of the most dangerous invasive species. Its worldwide spread has created health concerns as it is a major vector of arboviruses of public health significance such as chikungunya (CHIKV). Dynamics of different genetic backgrounds and admixture events may have impacted competence for CHIKV in adventive populations. Using microsatellites, we infer the genetic structure

    更新日期:2020-06-24
  • The genome of pest Rhynchophorus ferrugineus reveals gene families important at the plant-beetle interface
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-24
    Khaled Michel Hazzouri; Naganeeswaran Sudalaimuthuasari; Biduth Kundu; David Nelson; Mohammad Ali Al-Deeb; Alain Le Mansour; Johnston J. Spencer; Claude Desplan; Khaled M. A. Amiri

    The red palm weevil, Rhynchophorus ferrugineus, infests palm plantations, leading to large financial losses and soil erosion. Pest-host interactions are poorly understood in R. ferrugineus, but the analysis of genetic diversity and pest origins will help advance efforts to eradicate this pest. We sequenced the genome of R. ferrugineus using a combination of paired-end Illumina sequencing (150 bp),

    更新日期:2020-06-24
  • The iron–sulphur cluster in human DNA2 is required for all biochemical activities of DNA2
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-23
    Laura Mariotti; Sebastian Wild; Giulia Brunoldi; Alessandra Piceni; Ilaria Ceppi; Sandra Kummer; Richard E. Lutz; Petr Cejka; Kerstin Gari

    The nuclease/helicase DNA2 plays important roles in DNA replication, repair and processing of stalled replication forks. DNA2 contains an iron-sulphur (FeS) cluster, conserved in eukaryotes and in a related bacterial nuclease. FeS clusters in DNA maintenance proteins are required for structural integrity and/or act as redox-sensors. Here, we demonstrate that loss of the FeS cluster affects binding

    更新日期:2020-06-23
  • Author Correction: Mutually suppressive roles of KMT2A and KDM5C in behaviour, neuronal structure, and histone H3K4 methylation.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-22
    Christina N Vallianatos,Brynne Raines,Robert S Porter,Katherine M Bonefas,Michael C Wu,Patricia M Garay,Katie M Collette,Young Ah Seo,Yali Dou,Catherine E Keegan,Natalie C Tronson,Shigeki Iwase

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-06-22
  • Mapping CRISPR spaceromes reveals vast host-specific viromes of prokaryotes.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-22
    Sergey A Shmakov,Yuri I Wolf,Ekaterina Savitskaya,Konstantin V Severinov,Eugene V Koonin

    CRISPR arrays contain spacers, some of which are homologous to genome segments of viruses and other parasitic genetic elements and are employed as portion of guide RNAs to recognize and specifically inactivate the target genomes. However, the fraction of the spacers in sequenced CRISPR arrays that reliably match protospacer sequences in genomic databases is small, leaving the question of the origin(s)

    更新日期:2020-06-22
  • #Black Lives Matter.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-22

    Communications Biology is committed to improving diversity in our pages, our reviewer pool, and our editorial board. We recognize both the overt and unconscious racism in the sciences and have tasked ourselves with using our platform to amplify Black voices and the voices of all biologists of color.

    更新日期:2020-06-22
  • Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-19
    Jonas Nørskov Søndergaard,Keyi Geng,Christian Sommerauer,Ionut Atanasoai,Xiushan Yin,Claudia Kutter

    With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in this procedure is the efficient delivery of foreign nucleic acids into cells by cellular transfection. Since the vectors encoding the components necessary for CRISPR/Cas genome engineering are always large (9–19 kb)

    更新日期:2020-06-19
  • Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-19
    Connor J Cooper,Kaiyuan Zheng,Katherine W Rush,Alexander Johs,Brian C Sanders,Georgios A Pavlopoulos,Nikos C Kyrpides,Mircea Podar,Sergey Ovchinnikov,Stephen W Ragsdale,Jerry M Parks

    Bacteria and archaea possessing the hgcAB gene pair methylate inorganic mercury (Hg) to form highly toxic methylmercury. HgcA consists of a corrinoid binding domain and a transmembrane domain, and HgcB is a dicluster ferredoxin. However, their detailed structure and function have not been thoroughly characterized. We modeled the HgcAB complex by combining metagenome sequence data mining, coevolution

    更新日期:2020-06-19
  • Soil nitrogen concentration mediates the relationship between leguminous trees and neighbor diversity in tropical forests.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-19
    Han Xu,Matteo Detto,Suqin Fang,Robin L Chazdon,Yide Li,Billy C H Hau,Gunter A Fischer,George D Weiblen,J Aaron Hogan,Jess K Zimmerman,Maria Uriarte,Jill Thompson,Juyu Lian,Ke Cao,David Kenfack,Alfonso Alonso,Pulchérie Bissiengou,Hervé Roland Memiaghe,Renato Valencia,Sandra L Yap,Stuart J Davies,Xiangcheng Mi,Tze Leong Yao

    Legumes provide an essential service to ecosystems by capturing nitrogen from the atmosphere and delivering it to the soil, where it may then be available to other plants. However, this facilitation by legumes has not been widely studied in global tropical forests. Demographic data from 11 large forest plots (16–60 ha) ranging from 5.25° S to 29.25° N latitude show that within forests, leguminous trees

    更新日期:2020-06-19
  • Genomic signatures of domestication in Old World camels.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-19
    Robert Rodgers Fitak,Elmira Mohandesan,Jukka Corander,Adiya Yadamsuren,Battsetseg Chuluunbat,Omer Abdelhadi,Abdul Raziq,Peter Nagy,Chris Walzer,Bernard Faye,Pamela Anna Burger

    Domestication begins with the selection of animals showing less fear of humans. In most domesticates, selection signals for tameness have been superimposed by intensive breeding for economical or other desirable traits. Old World camels, conversely, have maintained high genetic variation and lack secondary bottlenecks associated with breed development. By re-sequencing multiple genomes from dromedaries

    更新日期:2020-06-19
  • Dissecting myogenin-mediated retinoid X receptor signaling in myogenic differentiation.
    Commun. Biol. (IF 4.165) Pub Date : 2020-06-18
    Saadia Khilji,Munerah Hamed,Jihong Chen,Qiao Li

    Deciphering the molecular mechanisms underpinning myoblast differentiation is a critical step in developing the best strategy to promote muscle regeneration in patients suffering from muscle-related diseases. We have previously established that a rexinoid x receptor (RXR)-selective agonist, bexarotene, enhances the differentiation and fusion of myoblasts through a direct regulation of MyoD expression

    更新日期:2020-06-18
Contents have been reproduced by permission of the publishers.
导出
全部期刊列表>>
材料学研究精选
Springer Nature Live 产业与创新线上学术论坛
胸腔和胸部成像专题
自然科研论文编辑服务
ACS ES&T Engineering
ACS ES&T Water
屿渡论文,编辑服务
杨超勇
周一歌
华东师范大学
段炼
清华大学
中科大
唐勇
跟Nature、Science文章学绘图
隐藏1h前已浏览文章
中洪博元
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
x-mol收录
福州大学
南京大学
王杰
左智伟
电子显微学
何凤
洛杉矶分校
吴杰
赵延川
试剂库存
天合科研
down
wechat
bug