To elevate the concentration of racemic styrene oxide (rac-SO), its asymmetric hydrolysis in organic/aqueous biphasic system was researched. Firstly, n-octane/phosphate buffer reaction system was constructed, where PvEH2 retained 75.9% activity at 25 °C for 8 h. Secondly, the optimal volume ratio of two phases and weight ratio of rac-SO to E. coli/pveh2 were 4:6 and 0.8. Then, the maximum allowable rac-SO concentration was assayed as 200 mM, 10-fold that in aqueous phase. Finally, the hydrolysis of rac-SO was performed, affording (R)-SO with over 99.5% ee_s and 47.8% yield, and (R)-phenyl-1,2-ethanediol with 93.6% ee_p and 50.4% yield in 12 h.

为了提高外消旋苯乙烯氧化物（rac -SO）的浓度，研究了其在有机/水两相体系中的不对称水解。首先，构建了正辛烷/磷酸盐缓冲液反应系统，其中Pv EH2在25°C下保持85.9％的活性。其次，两相的最佳体积比和rac -SO与大肠杆菌/ pveh2的重量比分别为4：6和0.8。然后，测定最大允许rac -SO浓度为200 mM，是水相的10倍。最后，进行rac -SO的水解，得到（R）-SO具有超过99.5％ee _s产率为47.8％，（R）-苯基-1,2-乙二醇在12小时内的ee _p为93.6％，产率为50.4％。