Localized information on a specimen is considered indispensable for deciphering biological activity. Magnetic resonance spectroscopy is a notable method because of its versatility; however, one limitation is the spectral quality on a static sample. This study explores an amalgamated method with two magnetic resonance experiments: high-resolution magic-angle spinning (HR-MAS) for high-quality spectral acquisition from a spinning sample and chemical shift imaging (CSI) for spatial localization. The advantage of HR-MAS CSI is its amenity for simultaneously profiling the metabolome—with good spectral data—at different spatial regions in a single experiment. Herein, ¹H HR-MAS CSI (including a T₂-contrast CSI) was described and performed on various food tissues and an intact organism. Different data analyses such as multivariate and quantification were explored to identify the metabolic variants in different anatomical regions and in one case, to assist in a spatial allocation. The limitation and drawback of the experiment are also discussed.

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标本上的局部信息被认为是解密生物活性必不可少的。磁共振波谱学由于其多功能性而成为一种值得注意的方法。然而，一个限制是静态样品的光谱质量。这项研究通过两个磁共振实验探索了一种融合方法：用于从旋转样本中获取高质量光谱的高分辨率魔角旋转（HR-MAS）和用于空间定位的化学位移成像（CSI）。HR-MAS CSI的优势在于它易于在单个实验中同时在不同空间区域对具有良好光谱数据的代谢组进行分析。此处，¹ H HR-MAS CSI（包括T ₂-对比度CSI），并在各种食物组织和完整的生物体上进行了描述。探索了不同的数据分析，例如多变量和定量分析，以识别不同解剖区域的代谢变异，在一种情况下，有助于空间分配。还讨论了实验的局限性和缺点。

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