Biofilms at the tooth-restoration bonded interface can produce acids and cause recurrent caries. Recurrent caries is a primary reason for restoration failures. The objectives of this study were to synthesize a novel bioactive dental bonding agent containing dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacryloyloxyethyl phosphorylcholine (MPC) to inhibit biofilm formation at the tooth-restoration margin and to investigate the effects of water-aging for 6 months on the dentin bond strength and protein-repellent and antibacterial durability. A protein-repellent agent (MPC) and antibacterial agent (DMAHDM) were added to a Scotchbond multi-purpose (SBMP) primer and adhesive. Specimens were stored in water at 37 °C for 1, 30, 90, or 180 days (d). At the end of each time period, the dentin bond strength and protein-repellent and antibacterial properties were evaluated. Protein attachment onto resin specimens was measured by the micro-bicinchoninic acid approach. A dental plaque microcosm biofilm model was used to test the biofilm response. The SBMP + MPC + DMAHDM group showed no decline in dentin bond strength after water-aging for 6 months, which was significantly higher than that of the control (P < 0.05). The SBMP + MPC + DMAHDM group had protein adhesion that was only 1/20 of that of the SBMP control (P < 0.05). Incorporation of MPC and DMAHDM into SBMP provided a synergistic effect on biofilm reduction. The antibacterial effect and resistance to protein adsorption exhibited no decrease from 1 to 180 d (P > 0.1). In conclusion, a bonding agent with MPC and DMAHDM achieved a durable dentin bond strength and long-term resistance to proteins and oral bacteria. The novel dental bonding agent is promising for applications in preventive and restorative dentistry to reduce biofilm formation at the tooth-restoration margin.

牙齿修复体粘合界面处的生物膜会产生酸并导致龋齿复发。复发性龋齿是修复失败的主要原因。本研究的目的是合成一种含有甲基丙烯酸二甲氨基十六酯 (DMAHDM) 和 2-甲基丙烯酰氧基乙基磷酰胆碱 (MPC) 的新型生物活性牙科粘合剂，以抑制牙齿修复体边缘生物膜的形成，并研究 6 个月水老化的影响牙本质粘合强度和蛋白质排斥性和抗菌耐久性。Scotchbond 多用途 (SBMP) 底漆和粘合剂中添加了蛋白质排斥剂 (MPC) 和抗菌剂 (DMAHDM)。样本在 37°C 的水中储存 1、30、90 或 180 天 (d)。在每个时间段结束时，评估牙本质粘合强度以及蛋白质排斥和抗菌性能。通过微二辛可宁酸方法测量树脂样本上的蛋白质附着。使用牙菌斑微观生物膜模型来测试生物膜反应。SBMP+MPC+DMAHDM组水老化6个月后牙本质粘结强度未见下降，显着高于对照组（P  <0.05）。SBMP + MPC + DMAHDM 组的蛋白质粘附仅为 SBMP 对照组的 1/20（P  < 0.05）。将 MPC 和 DMAHDM 掺入 SBMP 中可对减少生物膜产生协同效应。1~180 d抗菌效果和抗蛋白质吸附能力均无下降（P  >0.1）。总之，含有 MPC 和 DMAHDM 的粘结剂实现了持久的牙本质粘结强度以及对蛋白质和口腔细菌的长期抵抗力。这种新型牙科粘合剂有望在预防和修复牙科领域得到应用，以减少牙齿修复边缘生物膜的形成。