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Genetic analysis of the RNA polymerase II CTD in Drosophila
Methods ( IF 4.8 ) Pub Date : 2019-04-01 , DOI: 10.1016/j.ymeth.2019.01.010
Feiyue Lu 1 , David S Gilmour 2
Affiliation  

The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.

中文翻译:

果蝇中 RNA 聚合酶 II CTD 的遗传分析

RNA 聚合酶 II (Pol II) 的羧基末端结构域 (CTD) 在调节真核生物的基因表达中起重要作用。在这里,我们描述了用于研究果蝇 CTD 的多种遗传方法,这些方法补充了其他实验模型中对 Pol II CTD 的预先存在的分子分析。这些方法将允许人们评估任何 CTD 突变在发育复杂的生物体中的影响。这项工作中讨论的方法原则上可以应用于分析真核生物中的其他转录成分。
更新日期:2019-04-01
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