Calcium silicate-based cements (CSCs) are commonly used for endodontic procedures; however, their antibacterial effects are limited. The objective of this study was to develop a 2-methacryloyloxyethyl phosphorylcholine (MPC)-incorporated CSC with improved antibacterial properties, while maintaining the original advantageous features of CSC. MPC was incorporated into a commercial CSC (Endocem MTA) at 0 wt% (control), 1.5%, 3.0 wt%, 5.0 wt%, 7.5 wt%, and 10 wt%. The setting time, compressive strength, water sorption, and glycerol contact angle were measured. Protein absorption was measured and bacterial adhesion on the surface was evaluated using Enterococcus faecalis. The bactericidal effect was examined by the disc diffusion test. Mineralization ability was assessed based on calcium ion deposition, as assessed by alizarin red staining, after immersion into Hank’s balanced salt solution for 7 days. High concentrations of MPC in CSC (7.5 wt% and 10 wt%) increased the setting time, reduced compressive strength, and reduced wettability. MPC (3 wt%) had greater protein repellent and anti-biofouling effects than those of control and test materials (P < 0.001). However, no bactericidal effect was observed for any control or test materials. There was greater calcium ion deposition on the surface of MPC-supplemented CSC than on the control (P < 0.001). The addition of 3 wt% MPC polymer to CSC confers protein-repellent properties and reduced bacterial attachment, with the potential for improved mineralization.

硅酸钙基水泥（CSC）通常用于牙髓手术。但是，它们的抗菌作用是有限的。这项研究的目的是开发一种结合了2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）的CSC，具有改善的抗菌性能，同时保持CSC的原始优势。将MPC以0重量％（对照），1.5重量％，3.0重量％，5.0重量％，7.5重量％和10重量％掺入市售CSC（Endocem MTA）中。测量凝固时间，抗压强度，吸水率和甘油接触角。使用粪肠球菌测量蛋白质的吸收并评估其在表面的细菌粘附。通过盘片扩散测试检查杀菌效果。浸入Hank的平衡盐溶液中7天后，根据钙离子沉积（通过茜素红染色评估）评估了矿化能力。CSC中高浓度的MPC（7.5 wt％和10 wt％）会增加凝固时间，降低抗压强度，并降低润湿性。MPC（3 wt％）具有比对照材料和测试材料更大的蛋白质排斥力和抗生物结垢效果（P <0.001）。然而，对于任何对照或测试材料均未观察到杀菌作用。补充了MPC的CSC表面的钙离子沉积量大于对照组（P<0.001）。向CSC中添加3 wt％的MPC聚合物可赋予蛋白质排斥特性并减少细菌附着，并具有改善矿化的潜力。