High‐quality and disease‐resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgene‐free homozygous triple tms5/pi21/xa13 mutants obtained in the T₁ generation displayed characteristics of thermosensitive genic male sterility (TGMS) with enhanced resistance to rice blast and bacterial blight. Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing, which could significantly accelerate the breeding of sterile lines.

中文翻译：

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通过多重基因编辑开发抗病热敏雄性不育水稻。

高质量和抗病的雄性不育系在杂交水稻育种中具有巨大的应用潜力。我们使用CRISPR / Cas9多重基因组编辑系统，在品站中间育种材料中的TMS5，Pi21和Xa13基因中引入了特定的突变。我们发现，在T ₁代中获得的无转基因纯合子三重tms5 / pi21 / xa13突变体表现出热敏性雄性不育（TGMS）的特征，并增强了对稻瘟病和白叶枯病的抵抗力。本研究为通过多重基因编辑将育种中间材料转化为TGMS系提供了方便有效的途径，可大大促进不育系的育种。