The syntrophic photosynthesis via direct interspecies electron transfer (DIET) between Geobacter sulfurreducens and Prosthecochloris aestuarii has opened a new paradigm of microbial phototrophy. However, it is still unknown whether this photosynthetic DIET can be mediated by an electrical conductor. Here we report first the photosynthetic DIET in a two-chamber microbial fuel cell (photo-MFC). The photo-MFC worked well and generated a maximum current of 0.6 mA/m², which validated photosynthetic DIET via the titanium wire. Confocal laser scanning microscopy showed that G. sulfurreducens and P. aestuarii colonized the anode and cathode, respectively. P. aestuarii accepted extracellular electrons from G. sulfurreducens bioanodes under illumination at a current density of 0.6 mA/m² (normalized to the cathode surface area), which could not be produced in the dark. Such a light-dependent electron uptake from solid electrodes by P. aestuarii was closely related with the presence of CO₂, suggesting that P. aestuarii utilized extracellular electrons for photosynthetic CO₂ reduction. Electrochemical in situ Fourier transform infrared (FTIR) spectroscopy revealed that certain outer membrane components of the two strains were involved in the DIET process. These results implied photosynthetic DIET can be mediated by electrically conductive materials in natural environments.

通过直接种间的互养光合作用电子之间转移（DIET）地杆菌硫还原和Prosthecochloris aestuarii开辟微生物光营养的一个新的范例。然而，仍然不知道这种光合作用的DIET是否可以通过电导体来介导。在这里，我们首先报告两腔室微生物燃料电池（photo-MFC）中的光合作用DIET。光MFC工作良好，产生的最大电流为0.6 mA / m ²，通过钛丝验证了光合作用的DIET。共聚焦激光扫描显微镜观察表明，G。sulfreducens和P. aestuarii分别在阳极和阴极定居。南美白对虾在0.6 mA / m ²的电流密度（归一化为阴极表面积）的光照下，硫磺芽孢杆菌能够吸收来自氧化还原菌的生物阳极，这在黑暗中是无法产生的。假单胞菌从固体电极吸收的这种光依赖性电子与CO ₂的存在密切相关，这表明假单胞菌利用细胞外电子来光合作用CO _2。减少。电化学原位傅里叶变换红外（FTIR）光谱显示，这两种菌株的某些外膜成分都参与了DIET过程。这些结果暗示光合作用的DIET可以由自然环境中的导电材料介导。