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Temporal resistance of potato tubers: Antibacterial assays and metabolite profiling of wound-healing tissue extracts from contrasting cultivars
Phytochemistry ( IF 3.8 ) Pub Date : 2019-03-01 , DOI: 10.1016/j.phytochem.2018.12.007
Keyvan Dastmalchi 1 , Mathiu Perez Rodriguez 1 , Janni Lin 1 , Barney Yoo 2 , Ruth E Stark 3
Affiliation  

Solanum tuberosum, commonly known as the potato, is a worldwide food staple. During harvest, storage, and distribution the crop is at risk of mechanical damage. Wounding of the tuber skin can also become a point of entry for bacterial and fungal pathogens, resulting in substantial agricultural losses. Building on the proposal that potato tubers produce metabolites to defend against microbial infection during early stages of wound healing before protective suberized periderm tissues have developed, we assessed extracts of wound tissues from four potato cultivars with differing skin morphologies (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold). These assays were conducted at 0, 1, 2, 3 and 7 days post wounding against the plant pathogen Erwinia carotovora and a non-pathogenic Escherichia coli strain that served as a control. For each of the potato cultivars, only polar wound tissue extracts demonstrated antibacterial activity. The polar extracts from earlier wound-healing time points (days 0, 1 and 2) displayed notably higher antibacterial activity against both strains than the later wound-healing stages (days 3 and 7). These results support a burst of antibacterial activity at early time points. Parallel metabolite profiling of the extracts revealed differences in chemical composition at different wound-healing time points and allowed for identification of potential marker compounds according to healing stage for each of the cultivars. It was possible to monitor the transformations in the metabolite profiles that could account for the phenomenon of temporal resistance by looking at the relative quantities of various metabolite classes as a function of time.

中文翻译:

马铃薯块茎的时间抗性:来自对比栽培品种的伤口愈合组织提取物的抗菌测定和代谢物分析

马铃薯,俗称马铃薯,是世界范围内的主食。在收获、储存和分配过程中,作物面临机械损伤的风险。块茎皮肤的伤口也可能成为细菌和真菌病原体的进入点,导致大量农业损失。基于马铃薯块茎在伤口愈合的早期阶段产生代谢物以防御微生物感染的提议,在保护性松木化周皮组织发育之前,我们评估了来自具有不同皮肤形态的四种马铃薯品种(Norkotah Russet、Atlantic、Chipeta、和育空黄金)。这些测定是在伤后 0、1、2、3 和 7 天针对植物病原体欧文氏菌和作为对照的非致病性大肠杆菌菌株进行的。对于每个马铃薯品种,只有极地伤口组织提取物表现出抗菌活性。与伤口愈合后期(第 3 天和第 7 天)相比,早期伤口愈合时间点(第 0、1 和 2 天)的极性提取物对两种菌株的抗菌活性明显更高。这些结果支持在早期时间点的抗菌活性爆发。提取物的平行代谢物分析揭示了不同伤口愈合时间点化学成分的差异,并允许根据每个栽培品种的愈合阶段鉴定潜在的标记化合物。通过观察作为时间函数的各种代谢物类别的相对数量,可以监测可以解释时间阻力现象的代谢物谱中的转化。
更新日期:2019-03-01
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