Time-lapse fluorescence microscopy is a powerful tool to study gene regulation. By probing fluorescent signals in single cells over extended period of time, this method can be used to study the dynamics, noise, movement, memory, inheritance, and coordination, of gene expression during cell growth, development, and differentiation. In combination with a flow-cell device, it can also measure gene regulation by external stimuli. Due to the single cell nature and the spatial/temporal capacity, this method can often provide information that is hard to get using other methods. Here, we review the standard experimental procedures and new technical developments in this field.

中文翻译：

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使用延时荧光显微镜研究基因调控

延时荧光显微镜是研究基因调控的有力工具。通过长时间探测单个细胞中的荧光信号，该方法可用于研究细胞生长、发育和分化过程中基因表达的动力学、噪声、运动、记忆、遗传和协调。结合流动池装置，它还可以测量外部刺激的基因调控。由于单细胞性质和空间/时间容量，这种方法通常可以提供使用其他方法难以获得的信息。在这里，我们回顾了该领域的标准实验程序和新技术发展。