Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis.

的植物抗病性（基于地图的克隆- [R ）基因是耗时的。在这里，我们报告了使用比较转录组分析和全基因组序列分析的blast R基因Pid4的分离。Pid4编码卷曲螺旋核苷酸结合位点富含亮氨酸的重复（CC-NBS-LRR）蛋白，并在水稻品种Digu的不同发育阶段组成性表达。Pid4蛋白位于细胞核和细胞质中。将Pid4引入易感水稻品种后，可赋予种族特定的抗叶和颈部稻瘟病的能力。氨基酸序列比较和抗瘟谱测试表明Pid4是一种新型R该基因不同于先前报道的位于同一基因簇中的R基因。一个PID4个Indel标记的开发是为了便于识别PID4在不同的水稻品种。我们证明了可以使用转录组分析结合全基因组序列分析来快速分离植物R基因。