This study presents evaluation of the possible interaction mechanism between calf thymus dsDNA and three calcium antagonists; nifedipine, lercanidipine and amlodipine. The interactions between Nifedipine-dsDNA and Lercanidipine-dsDNA were investigated by differential pulse voltammetry using two different interaction methods; at the dsDNA-electrochemical biosensor surface and in bulk incubated solution. Amlodipine was used as model drug in bulk incubated solution. The decrease in the peak current of guanine and adenine were used as an indicator for confirmation of the interaction event in acetate buffer of pH 4.70. In bulk incubated solution, after interaction with Nifedipine and Amlodipine the guanine signal was almost disappeared. At the dsDNA modified glassy carbon electrode surface, the peak currents of guanine and adenine were decreased while Nifedipine and Lercanidipine interacts with DNA. The interactions between Nifedipine-dsDNA and Lercanidipine-dsDNA were further studied by UV–Vis absorption spectroscopy which indicates the intermolecular interaction between these drugs and ds-DNA can be mainly through hydrogen bonding and van der Waals forces. Molecular docking calculations shown that the AMP-1-2, NDP and LDP-1-2-ctDNA having groove binding. Beside spectral data, docking studies elicited that AMP-1-2, NDP and LDP-1-2 complexes have different interaction and conformation trends to target (ctDNA).

这项研究提出了小牛胸腺dsDNA与三种钙拮抗剂之间可能相互作用的机制的评估。硝苯地平，乐卡地平和氨氯地平。使用两种不同的相互作用方法，通过差示脉冲伏安法研究了硝苯地平-dsDNA和乐卡地平-dsDNA之间的相互作用。在dsDNA-电化学生物传感器表面和大量孵育溶液中。氨氯地平在大量孵育溶液中用作模型药物。鸟嘌呤和腺嘌呤的峰值电流的降低用作确认pH为4.70的乙酸盐缓冲液中相互作用事件的指示剂。在大量温育溶液中，与硝苯地平和氨氯地平相互作用后，鸟嘌呤信号几乎消失了。在dsDNA修饰的玻碳电极表面，硝苯地平和乐卡地平与DNA相互作用时，鸟嘌呤和腺嘌呤的峰值电流降低。紫外-可见吸收光谱进一步研究了硝苯地平-dsDNA和乐卡地平-dsDNA之间的相互作用，这表明这些药物与ds-DNA之间的分子间相互作用主要是通过氢键和范德华力。分子对接计算表明，AMP-1-2，NDP和LDP-1-2-ctDNA具有凹槽结合。除光谱数据外，对接研究还发现AMP-1-2，NDP和LDP-1-2复合物对靶标（ctDNA）的相互作用和构象趋势不同。紫外-可见吸收光谱进一步研究了硝苯地平-dsDNA和乐卡地平-dsDNA之间的相互作用，这表明这些药物与ds-DNA之间的分子间相互作用主要是通过氢键和范德华力。分子对接计算表明，AMP-1-2，NDP和LDP-1-2-ctDNA具有凹槽结合。除光谱数据外，对接研究还发现AMP-1-2，NDP和LDP-1-2复合物对靶标（ctDNA）的相互作用和构象趋势不同。紫外-可见吸收光谱进一步研究了硝苯地平-dsDNA和乐卡地平-dsDNA之间的相互作用，这表明这些药物与ds-DNA之间的分子间相互作用主要是通过氢键和范德华力。分子对接计算表明，AMP-1-2，NDP和LDP-1-2-ctDNA具有凹槽结合。除光谱数据外，对接研究还发现AMP-1-2，NDP和LDP-1-2复合物对靶标（ctDNA）的相互作用和构象趋势不同。