Biochimie ( IF 3.9 ) Pub Date : 2018-12-14 , DOI: 10.1016/j.biochi.2018.12.007 Juliana T. Clemente-Napimoga , Maria A.S.M. Silva , Sylvia N.C. Peres , Alexandre H.P. Lopes , Claudia F. Lossio , Messias V. Oliveira , Vinicius J.S. Osterne , Kyria S. Nascimento , Henrique B. Abdalla , Juliana M. Teixeira , Benildo S. Cavada , Marcelo H. Napimoga
Inflammation of temporomandibular joint (TMJ) tissues are the most common cause of pain conditions associated with temporomandibular disorders (TMDs). After a tissue and/or neural damage, the inflammatory response is characterized by plasma extravasation and leukocytes infiltration in the TMJ tissues, which in turn, release inflammatory cytokines cascades responsible for inflammatory pain. Lectins are glycoproteins widely distributed in nature that may exhibit anti-inflammatory properties. This study demonstrated by molecular docking and MM/PBSA that the lectin from Dioclea violacea (DVL) interacts favorably with α-methyl-D-mannoside, N-acetyl-D-glucosamine, and core1-sialyl-Lewis X which are associated with leukocytes migration during an inflammatory response. Wistar rats pretreated with intravenously injection of DVL demonstrated a significant inhibition of plasma extravasation induced by carrageenan (a non-neurogenic inflammatory inductor) and mustard oil (a neurogenic inflammatory inductor) in the TMJ periarticular tissues (p < 0.05; ANOVA, Tukey's test). In addition, DVL significantly reduced carrageenan-induced leukocyte migration in the TMJ periarticular tissues mediated by down-regulation of ICAM-1 expression. These results suggest a potential anti-inflammatory effect of DVL in inflammatory conditions of TMJ.
中文翻译:
紫荆花凝集素通过抑制细胞间黏附分子-1的表达改善大鼠颞下颌关节的炎症
颞下颌关节(TMJ)组织发炎是与颞下颌疾病(TMD)相关的疼痛症状的最常见原因。在组织和/或神经损伤后,炎性反应的特征在于血浆外渗和TMJ组织中的白细胞浸润,继而释放引起炎性疼痛的炎性细胞因子级联反应。凝集素是自然界中广泛分布的糖蛋白,可表现出抗炎特性。这项研究通过分子对接和MM / PBSA证明了紫丁香(Dioclea violacea)(DVL)中的凝集素与α-甲基-D-甘露糖苷,N-与白细胞在炎症反应期间迁移有关的乙酰基-D-氨基葡萄糖和core1-唾液酸化-刘易斯X。静脉注射DVL预处理的Wistar大鼠表现出显着抑制TMJ关节周围组织中角叉菜胶(一种非神经源性炎症诱导剂)和芥子油(一种神经源性炎症诱导剂)诱导的血浆外渗(p <0.05; ANOVA,Tukey检验) 。此外,DVL显着降低了ICAM-1表达下调介导的角叉菜胶诱导的TMJ关节周围组织中白细胞迁移。这些结果表明DVL在TMJ的炎性病症中具有潜在的抗炎作用。