3-(7-(Diethylamino)-2-oxo-2H-chromen-3-yl)-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one (COPYR) was competently synthesized from methyl 3-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)-3-oxopropanoate and 2-hydrazino-4-phenyl thiazole. The interactions of receptor COPYR with various ions were investigated through absorption and steady-state fluorescence spectral studies, respectively. The results reveal that the complexation of Cr³⁺ ions with COPYR results in 1:1 stoichiometry, which resulted in a quick color response from fluorescent green to colorless with significant quenching of fluorescence at 506 nm in dimethyl sulfoxide medium. Theoretical calculations (density functional theory) confirm the photophysical changes such as absorbance and emission spectral changes. Furthermore, the confocal imaging of A-549 cells clearly points out that the synthesized probe COPYR could be applied for detecting Cr³⁺ in living cells.

中文翻译：

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Cr ^3+的高灵敏度香豆素–吡唑啉酮探针及其在活细胞中的应用

3-（7-（二乙氨基）-2-oxo-2 H -chromen-3-yl）-1-（4-phenylthiazol-2-yl）-1 H -pyrazol -5（4 H）-one（COPYR）被从胜任甲基合成3-（7-（二乙氨基）-2-氧代- 2 ħ -色烯-3-基）-3-氧代丙酸乙酯和2-肼基-4-苯基噻唑。分别通过吸收和稳态荧光光谱研究了受体COPYR与各种离子的相互作用。结果表明，Cr ³⁺的络合带有COPYR的离子导致化学计量比为1：1，从而导致了从荧光绿色到无色的快速颜色响应，并且在二甲基亚砜介质中在506 nm处的荧光显着猝灭。理论计算（密度泛函理论）证实了光物理变化，例如吸光度和发射光谱变化。此外，对A-549细胞的共聚焦成像清楚地指出，合成探针COPYR可用于检测活细胞中的Cr ³⁺。