The localization of acteoside and its precursors in different organs of sesame plant (Sesamum indicum L.), including roots, stems (epidermis, cortex, and stele), leaves (blades and petioles), flower petals, and seeds (immature and mature) was investigated. The highest acteoside content was found in leaf blades (12.3% dry weight), followed by in petioles (3.1%) and petals (2.7%) and at a trace levels or not detected in other organs. In contrast, the known precursors of acteoside, cinnamate, p-coumarate, caffeate, tyramine, tyrosol, salidroside, l-3,4-dihydroxyphenylalanine, dopamine, and hydroxytyrosol were not detected in any organs, even by multiple-reaction monitoring analysis by liquid chromatography–electrospray ionization tandem mass spectrometry, despite acteoside being present at high levels in the leaves. The leaves stained with diphenylborinic acid 2-aminoethyl ester (DPBA), producing the acteoside–DPBA complex that was detected by fluorescence at an excitation wavelength of 365 nm, showed accumulation of acteoside at the veins and glandular trichomes. For the first time, the leaf blade organ was shown to contain a large amount of acteoside in the sesame plant, with acteoside synthesis in the leaf petioles and/or blades and accumulation in the glandular trichomes with transport through the veins. However, tyrosine and phenylalanine contents were very low with no downstream metabolites or known acteoside precursors detected in any organs.

中文翻译：

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麦角甾苷的积累及亚细胞分布的植物芝麻（胡麻L.）

芝麻菜苷及其前体在芝麻植物（芝麻）的不同器官中的定位，包括根，茎（表皮，皮层和石碑），叶（叶片和叶柄），花瓣和种子（未成熟）被调查了。叶片中的洋紫苏苷含量最高（干重为12.3％），其次是叶柄（3.1％）和花瓣（2.7％），并且在其他器官中均未检出或未检出。相比之下，已知的有藻油苷，肉桂酸酯，对香豆酸酯，咖啡酸酯，酪胺，酪醇，红景天苷，l的已知前体。尽管在叶中存在高含量的鹅膏苷，但即使通过液相色谱-电喷雾串联质谱法进行多反应监测分析，也未在任何器官中检测到-3,4-二羟基苯丙氨酸，多巴胺和羟基酪醇。叶片被二苯基硼酸2-氨基乙基酯（DPBA）染色，产生了Acteoside-DPBA复合物，该荧光在365 nm的激发波长下被荧光检测到，表明Acteoside在静脉和腺毛上积累。首次显示出芝麻叶片中的叶片器官中含有大量的鹅膏苷，叶柄和/或叶片中的鹅膏苷合成并通过静脉运输而在腺毛中积累。然而，