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Spatial-temporal survey of Microcystis oligopeptide chemotypes in reservoirs with dissimilar waterbody features and their relation to genetic variation.
Harmful Algae ( IF 6.6 ) Pub Date : 2018-12-11 , DOI: 10.1016/j.hal.2018.11.009
M Á Lezcano 1 , R Agha 2 , S Cirés 1 , A Quesada 1
Affiliation  

The ability of cyanobacteria to produce toxins and other secondary metabolites is patchily distributed in natural populations, enabling the use of cellular oligopeptide compositions as markers to classify strains into ecologically-relevant chemotypical subpopulations. The composition and spatiotemporal distribution of Microcystis chemotypes within and among waterbodies was studied at different time scales by analyzing (i) Microcystis strains isolated between 1998 and 2007 from different Spanish reservoirs and (ii) individual Microcystis aeruginosa colonies collected from pelagic and littoral habitats in Valmayor reservoir (Spain) during a bloom. No agreement between chemotypes and both morphotypes and genotypes (based on cpcBA-IGS, 16S–23S rRNA ITS and mcyB genes) was found, suggesting that oligopeptide profiles in individual strains evolve independently across morphospecies and phylogenetic genotypes, and that the diversity of microcystin variants produced cannot be explained by mcyB gene variations alone. The presence of identical chemotypes in spatially-distant reservoirs with dissimilar trophic state, lithology or depth indicate that waterbody characteristics and geographical boundaries weakly affect chemotype composition and distribution. At smaller spatiotemporal scales (i.e. during bloom), M. aeruginosa populations showed high number of chemotypes, as well as marked differences in chemotype composition and relative abundance among the littoral and pelagic habitats. This indicates that the factors influencing chemotype composition, relative abundance and dynamics operate at short spatial and temporal scales, and supports emerging hypotheses about interactions with antagonistic microorganisms as possible drivers for widespread chemical polymorphisms in cyanobacteria.



中文翻译:

具有不同水体特征的水库中微囊藻寡肽化学型的时空调查及其与遗传变异的关系。

蓝细菌产生毒素和其他次生代谢产物的能力在自然种群中分布不一,从而可以使用细胞寡肽组合物作为标记,将菌株分类为与生态相关的化学典型亚群。通过分析(i)1998年至2007年间从西班牙不同水库中分离出的微囊藻菌株以及(ii)从瓦尔马约尔中上层和滨海生境收集到的单个铜绿微囊藻菌落,研究了不同时间尺度内水体内和间微囊藻化学型的组成和时空分布。水库(西班牙)在开花期间。化学型与形态型和基因型之间均不一致(基于cpcBA-IGS,16S–23S rRNA ITS和mcy B基因)被发现,这表明单个菌株中的寡肽谱在形态学和系统发育基因型之间独立发展,并且所产生的微囊藻毒素变异体的多样性不能仅通过mcy B基因变异来解释。在具有不同营养状态,岩性或深度的空间遥远的储层中存在相同的化学型,这表明水体特征和地理边界对化学型的组成和分布具有微弱的影响。在较小的时空尺度上(即开花期间),铜绿假单胞菌种群显示出大量的化学型,以及沿海和中上层生境之间化学型组成和相对丰度的显着差异。这表明影响化学型组成,相对丰度和动力学的因素在短时空尺度上起作用,并且支持关于与拮抗微生物相互作用的新假设,这些假设可能是导致蓝细菌中广泛的化学多态性的驱动因素。

更新日期:2018-12-11
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