Eradication of P. putida F1 was investigated as a function of current density in pulsed electric fields of 6.7, 4, 2.8, 2 and 1 kV/cm. The pulse numbers were 200, 2000, 5000 and 10,000 and were performed by a series of trains of 500 pulses (except for the 200 pulses). The frequency was 100 Hz and pulse durations were 10 μs or 20 μs as indicated for each experiment. The current density range was 0.02 ± 0.01 to 5.2 ± 0.5 Acm⁻². A clear tendency for increasing bacterial death was found as a result of increasing the current density in each of the tested electric field strengths. The total bacterial eradication when the electric field was reduced from 4 to 1 kV/cm was obtained at a higher current density, 2 ± 0.2 and 5.2 ± 0.5 Acm⁻², respectively. Increasing the current density led to higher cell permeability and larger bacteria size. The percentage of propidium iodide permeability in an electric field of 1 kV/cm at a current density of 5.2 ± 0.5 Acm⁻² was 65 ± 0.3% compared to the control that was only 10 ± 0.9%. The cell size at 1 kV/cm in a current density of 5.2 ± 0.5 Acm⁻² was about 3-fold higher compared to untreated cells. To the best of our knowledge, this is the first study that evaluated the influence of increasing current density on bacterial eradication in moderate electric fields.

研究了恶臭假单胞菌F1的根除与6.7、4、2.8、2和1 kV / cm脉冲电场中电流密度的关系。脉冲数为200、2000、5000和10,000，并通过一系列500个脉冲的序列（除200个脉冲之外）执行。如每个实验所示，频率为100 Hz，脉冲持续时间为10μs或20μs。电流密度范围为0.02±0.01至5.2±0.5Acm ^-2。由于增加了每个测试电场强度中的电流密度，发现了明显的增加细菌死亡的趋势。在较高的电流密度（2±0.2和5.2±0.5 Acm ^-2）下，将电场从4 kV / cm降低到1 kV / cm时，细菌的总清除率达到了， 分别。电流密度的增加导致更高的细胞渗透性和更大的细菌大小。在电流为5.2±0.5 Acm ^-2的1 kV / cm的电场中，碘化丙啶的渗透性百分比为65±0.3％，而对照组仅为10±0.9％。与未经处理的电池相比，在电流密度为5.2±0.5 Acm ^-2的情况下，在1 kV / cm的电池尺寸约高3倍。据我们所知，这是第一项评估电流密度增加对中等电场中细菌根除的影响的研究。