The long-term benefits conferred by cardiac rehabilitation (CR) in those with coronary artery disease (CAD) are strongly linked with an improvement in cardiopulmonary fitness. This study aimed to determine the association between peripheral sphingolipids and cardiopulmonary fitness in CAD subjects undertaking CR. Patients with CAD (n = 100, mean age = 64 ± 6 years, 85% male, mean years of education = 17 ± 3 years) underwent 6 months of CR with blood collected at baseline, 3 and 6 months. Cardiopulmonary fitness was assessed by measuring peak oxygen uptake (VO_2peak) at all time points. High performance liquid chromatography coupled electrospray ionization tandem mass spectrometry was used to quantify plasma sphingolipid concentrations. Cross-sectional and longitudinal associations between sphingolipids and VO_2peak were assessed using linear regressions and mixed models, respectively. Higher concentrations of sphingomyelin C18:1 (β = −0.26, p = .01), ceramides C16:0 (β = −0.24, p = .02), C18:0 (β = −0.29, p = .002), C20:0 (β = −0.24, p = .02) and C24:1 (β = −0.24, p = .01) and monohexylceramide C18:0 (β = −0.23, p = .02) were associated with poorer VO_2peak at baseline. An improvement in VO_2peak was associated with a decrease in sphingomyelin C18:1 (b = −10.09, p = .006), ceramides C16:0 (b = −9.25, p = .0003), C18:0 (b = −5.44, p = .0003) and C24:1 (b = −2.46, p = .006) and monohexylceramide C18:0 (b = −5.37, p = .005). Specific long chain sphingolipids may be useful markers of fitness and response to exercise in CAD.

中文翻译：

---

冠状动脉疾病心脏康复患者鞘脂与心肺功能的关系

心脏康复 (CR) 对冠状动脉疾病 (CAD) 患者的长期益处与心肺健康的改​​善密切相关。本研究旨在确定进行 CR 的 CAD 受试者外周鞘脂与心肺健康之间的关联。CAD 患者（n = 100，平均年龄 = 64 ± 6 岁，85% 男性，平均受教育年限 = 17 ± 3 年）接受了 6 个月的 CR，并在基线、3 个月和 6 个月时采集血液。通过测量峰值摄氧量（VO _2peak) 在所有时间点。高效液相色谱耦合电喷雾电离串联质谱法用于量化血浆鞘脂浓度。分别使用线性回归和混合模型评估鞘脂和 VO _2peak之间的横截面和纵向关联。更高浓度的鞘磷脂 C18:1 (β = -0.26, p = .01), 神经酰胺 C16:0 (β = -0.24, p = .02), C18:0 (β = -0.29, p = .002), C20:0 (β = -0.24, p = .02) 和 C24:1 (β = -0.24, p = .01) 和单己基神经酰胺 C18:0 (β = -0.23, p = .02) 与较差的 VO 相关2 基线_峰值。VO的改进_2peak与鞘磷脂 C18:1 ( b = -10.09, p = .006)、神经酰胺 C16:0 ( b = -9.25, p = .0003)、C18:0 ( b = -5.44, p = .0003) 和 C24:1 ( b = -2.46, p = .006) 和单己基神经酰胺 C18:0 ( b = -5.37, p = .005)。特定的长链鞘脂可能是 CAD 中健康和对运动反应的有用标志物。