Many open questions in RNA biology relate to the kinetics of gene expression and the impact of RNA binding regulatory factors on processing or decay rates of particular transcripts. Steady state measurements of RNA abundance obtained from RNA-seq approaches are not able to separate the effects of transcription from those of RNA decay in the overall abundance of any given transcript, instead only giving information on the (presumed steady-state) abundances of transcripts. Through the combination of metabolic labeling and high-throughput sequencing, several groups have been able to measure both transcription rates and decay rates of the entire transcriptome of an organism in a single experiment. This review focuses on the methodology used to specifically measure RNA decay at a global level. By comparing and contrasting approaches and describing the experimental protocols in a modular manner, we intend to provide both experienced and new researchers to the field the ability to combine aspects of various protocols to fit the unique needs of biological questions not addressed by current methods.

中文翻译：

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使用生物正交标记结合下一代 RNA 测序对 RNA 代谢进行全局分析

RNA 生物学中的许多悬而未决的问题与基因表达的动力学和 RNA 结合调节因子对特定转录本的加工或衰减率的影响有关。从 RNA-seq 方法获得的 RNA 丰度的稳态测量无法将转录的影响与任何给定转录本的整体丰度中的 RNA 衰变的影响分开，而只能提供有关（假定的稳态）转录本丰度的信息. 通过代谢标记和高通量测序的结合，几个小组已经能够在一次实验中测量生物体整个转录组的转录率和衰减率。本综述重点介绍用于在全球范围内专门测量 RNA 衰变的方法。