Nowadays, smart polymers have been received a great attention in biomedical applications, especially in sensors because of their novel and peculiar properties. In this study, sphere-like structure of poly-N-isopropylacrylamide (PNIPAM) microgel was prepared via one-step precipitation polymerization method. Cyclic voltammetry and electrochemical impedance spectroscopy experiment showed that the PNIPAM microgel could enhance the electrochemical performance of the electrodes, as a consequence of the large surface area presented by the PNIPAM. The PNIPAM microgel modified glassy carbon electrode (PNIPAM microgel modified GCE) exhibited an enhanced electrocatalytic activity towards antipsychotic drug chlorpromazine (CPZ) detection. The electrochemical response of CPZ using PNIPAM microgel modified GCE was linear over the range from 0.05 to 7999 µM. The sensitivity and limit of detection were 0.084 µAµM⁻¹cm⁻² and 0.016 µM, respectively. In addition, the sensor is highly sensitive, selective and durable even in the presence of potentially interfering electroactive compounds. The good analytical performance of the PNIPAM microgel modified GCE was confirmed for determining trace level CPZ in human serum and urine samples with satisfactory results.

如今，智能聚合物因其新颖独特的性能而在生物医学应用中尤其是在传感器中受到了广泛的关注。本研究采用一步沉淀聚合法制备了聚N-异丙基丙烯酰胺（PNIPAM）微凝胶的球形结构。循环伏安法和电化学阻抗谱实验表明，由于PNIPAM具有较大的表面积，PNIPAM微凝胶可增强电极的电化学性能。PNIPAM微凝胶修饰的玻碳电极（PNIPAM微凝胶修饰的GCE）对抗精神病药物氯丙嗪（CPZ）检测表现出增强的电催化活性。使用PNIPAM微凝胶修饰的GCE的CPZ的电化学响应在0.05至7999 µM的范围内呈线性。^-1 cm ^-2和0.016 µM。此外，即使在潜在干扰的电活性化合物存在的情况下，该传感器也具有很高的灵敏度，选择性和耐用性。PNIPAM微凝胶修饰的GCE的良好分析性能可用于测定人血清和尿液样品中的痕量CPZ，结果令人满意。