Antinuclear antibodies (ANAs), antineutrophil cytoplasmic antibodies (ANCAs), rheumatoid factor (RF) and antibodies to citrullinated peptides (ACPA) are widely used laboratory markers to support the diagnosis of ANA-associated systemic rheumatic diseases, ANCA-associated vasculitis and rheumatoid arthritis. Despite the fact that these tests are broadly used, they all suffer from a lack of harmonisation. Pisetsky et al 1 recently reported on assay variation in the detection of ANAs in the sera of patients with established systemic lupus erythematosus. Differences in ANA reactivity were found between different indirect immunofluorescence (IIF) methods and between IIF and immunoassays. Infantino et al 2 further stressed (1) that such …

中文翻译：

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风湿病实验室检测的统一：还有很长的路要走

抗核抗体 (ANA)、抗中性粒细胞胞浆抗体 (ANCA)、类风湿因子 (RF) 和瓜氨酸肽抗体 (ACPA) 是广泛使用的实验室标志物，以支持 ANA 相关系统性风湿病、ANCA 相关血管炎和类风湿性关节炎的诊断. 尽管这些测试被广泛使用，但它们都缺乏协调性。Pisetsky 等人 1 最近报道了在已确诊的系统性红斑狼疮患者血清中检测 ANA 的测定变异。在不同的间接免疫荧光 (IIF) 方法之间以及 IIF 和免疫测定之间发现了 ANA 反应性的差异。Infantino 等人 2 进一步强调 (1) 这样的……