Global health issues are increasingly becoming critical with high fatality rate due to chronic and infectious diseases. Emerging technologies aim to address these problems by understanding the causes of lethal conditions and diagnosing symptoms at early stage. Existing commercial diagnostics primarily focus on single‐plex assays due to ease‐of‐use, simplicity in analysis, and amenability to mass manufacturing. Many research grade devices have utilized only a few molecular and morphological signatures in bodily fluids. However, multiplex devices can improve accuracy, sensitivity, and scalability of research and diagnostic devices. This review presents multiplex assays that utilize optical, electrical, and chemical methods and materials that have the potential to improve portable point‐of‐care diagnostics. The critical role of emerging optical and complementary assays with multiple contrast mechanisms is investigated to enable highly multiplex analysis in field settings. Multiparameter portable devices for field applications toward health monitoring, food testing, air quality monitoring, and microanalysis in other extreme conditions are examined. Current trends indicate the need for validation of health diagnosis based on a large number of biomarkers in randomized clinical trials. Advanced digital analysis, crowd‐sourced solutions, and robust user interfaces will become an integral part of the connected global health systems and personalized monitoring platforms.

中文翻译：

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便携式多重光学检测

由于慢性病和传染病导致的高死亡率，全球健康问题正变得越来越关键。新兴技术旨在通过了解致死性疾病的原因并在早期诊断症状来解决这些问题。由于易于使用，分析简单以及易于批量生产，因此现有的商业诊断程序主要集中在单重检测上。许多研究级设备仅在体液中利用了少数分子和形态特征。但是，多路复用设备可以提高研究和诊断设备的准确性，灵敏度和可伸缩性。这篇综述介绍了利用光，电，化学方法和材料进行的多重检测，这些检测方法和材料有可能改善便携式即时诊断。研究了新兴的具有多种对比机制的光学和互补测定的关键作用，以实现现场设置中的高度多重分析。对用于野外健康监测，食品测试，空气质量监测和其他极端条件下的微量分析的多参数便携式设备进行了检查。当前的趋势表明，需要在随机临床试验中基于大量生物标记物对健康诊断进行验证。先进的数字分析，众包解决方案和强大的用户界面将成为连接的全球卫生系统和个性化监控平台不可或缺的一部分。对用于野外健康监测，食品测试，空气质量监测和其他极端条件下的微量分析的多参数便携式设备进行了检查。当前的趋势表明，需要在随机临床试验中基于大量生物标记物对健康诊断进行验证。先进的数字分析，众包解决方案和强大的用户界面将成为连接的全球卫生系统和个性化监控平台不可或缺的一部分。对用于野外健康监测，食品测试，空气质量监测和其他极端条件下的微量分析的多参数便携式设备进行了检查。当前的趋势表明，需要在随机临床试验中基于大量生物标记物对健康诊断进行验证。先进的数字分析，众包解决方案和强大的用户界面将成为连接的全球卫生系统和个性化监控平台不可或缺的一部分。