Ribose methylation is one of the most abundant RNA modifications and is found in all domains of life and all major classes of RNA (rRNA, tRNA, and mRNA). Ribose methylations are introduced by stand-alone enzymes or by generic enzymes guided to the target by small RNA guides. Recent years have seen the development of several sequencing-based methods for RNA modifications relying on different principles. In this review, we compare mapping and quantitation studies of ribose methylations from yeast and human culture cells. The emphasis is on ribosomal RNA for which the results can be compared to results from RNA fingerprinting and mass spectrometry. One sequencing approach is consistent with these methods and paints a conservative picture of rRNA modifications. Other approaches detect many more sites. Similar discrepancies are found in measurements of modification stoichiometry. The results are discussed in relation to the more challenging task of mapping ribose methylations in mRNA.

中文翻译：

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用于检测和定量 RNA 中核糖甲基化的基于测序的方法

核糖甲基化是最丰富的 RNA 修饰之一，存在于生命的所有领域和所有主要类别的 RNA（rRNA、tRNA 和 mRNA）中。核糖甲基化由独立的酶或由小 RNA 向导引导到目标的通用酶引入。近年来，根据不同的原理，开发了几种基于测序的 RNA 修饰方法。在这篇综述中，我们比较了酵母和人类培养细胞核糖甲基化的作图和定量研究。重点是核糖体 RNA，其结果可以与 RNA 指纹图谱和质谱的结果进行比较。一种测序方法与这些方法一致，并描绘了 rRNA 修饰的保守图景。其他方法检测更多的站点。在改性化学计量的测量中发现了类似的差异。讨论了与映射 mRNA 中核糖甲基化这一更具挑战性的任务相关的结果。