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Oxidative stress in the oral cavity is driven by individual-specific bacterial communities
npj Biofilms and Microbiomes ( IF 9.2 ) Pub Date : 2018-11-27 , DOI: 10.1038/s41522-018-0072-3
Mária Džunková , Daniel Martinez-Martinez , Roman Gardlík , Michal Behuliak , Katarína Janšáková , Nuria Jiménez , Jorge F. Vázquez-Castellanos , Jose Manuel Martí , Giuseppe D’Auria , H. M. H. N. Bandara , Amparo Latorre , Peter Celec , Andrés Moya

The term “bacterial dysbiosis” is being used quite extensively in metagenomic studies, however, the identification of harmful bacteria often fails due to large overlap between the bacterial species found in healthy volunteers and patients. We hypothesized that the pathogenic oral bacteria are individual-specific and they correlate with oxidative stress markers in saliva which reflect the inflammatory processes in the oral cavity. Temporally direct and lagged correlations between the markers and bacterial taxa were computed individually for 26 volunteers who provided saliva samples during one month (21.2 ± 2.7 samples/volunteer, 551 samples in total). The volunteers’ microbiomes differed significantly by their composition and also by their degree of microbiome temporal variability and oxidative stress markers fluctuation. The results showed that each of the marker-taxa pairs can have negative correlations in some volunteers while positive in others. Streptococcus mutans, which used to be associated with caries before the metagenomics era, had the most prominent correlations with the oxidative stress markers, however, these correlations were not confirmed in all volunteers. The importance of longitudinal samples collections in correlation studies was underlined by simulation of single sample collections in 1000 different combinations which produced contradictory results. In conclusion, the distinct intra-individual correlation patterns suggest that different bacterial consortia might be involved in the oxidative stress induction in each human subject. In the future, decreasing cost of DNA sequencing will allow to analyze multiple samples from each patient, which might help to explore potential diagnostic applications and understand pathogenesis of microbiome-associated oral diseases.



中文翻译:

口腔中的氧化应激是由个体特异性细菌群落驱动的

在宏基因组学研究中,“细菌性营养不良”一词已被广泛使用,但是,由于健康志愿者和患者体内发现的细菌种类之间存在大量重叠,因此有害细菌的鉴定常常会失败。我们假设病原性口腔细菌是个体特异性的,它们与唾液中的氧化应激标志物相关,反映了口腔中的炎症过程。分别计算了26个志愿者在一个月内提供唾液样本(21.2±2.7个样本/志愿者,总共551个样本)的标记物和细菌类群之间的时间直接相关和滞后相关性。志愿者的微生物组的组成,微生物组的时间变异程度和氧化应激标志物的波动程度均存在显着差异。变形链球菌在宏基因组学时代之前曾与龋齿相关,与氧化应激标记物之间的关联最为明显,但是,并非所有志愿者都证实了这些相关性。纵向样本收集在相关性研究中的重要性通过模拟1000个不同组合中的单个样本收集来产生矛盾的结果来强调。总之,不同的个体内相关模式表明,在每个人类受试者中,不同的细菌群落可能参与了氧化应激的诱导。将来,DNA测序成本的降低将允许对每位患者的多个样品进行分析,这可能有助于探索潜在的诊断应用并了解与微生物组相关的口腔疾病的发病机理。

更新日期:2019-11-18
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