Many reports demonstrated that aluminum maltolate (Almal) has potential toxicity to human and animal. Our study has demonstrated that Almal can induce oxidative damage and apoptosis in PC12 cells and SH-SY5Y Cells, two in vitro models of neuronal cells. Hyperforin (HF) is a well-known antioxidant, anti-inflammatory, anti-amyloid and anti-depressant compound extracted from Hypericum perforatum extract. Here, we investigated the neuroprotective effect of HF against Almal-induced neurotoxicity in cultured PC12 cells and SH-SY5Y cells, mainly caused by oxidative stress. In the present study, HF significantly inhibited the formation of reactive oxygen species (ROS), decreased the level of lipid peroxide and enhanced the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) compared with Almal group in PC12 cells and SH-SY5Y cells. Additionally, HF suppressed the reduction of the mitochondrial membrane potential (MMP), cytochrome c (Cyt-c) release, activation of caspase-3, and the down-regulation of Bcl-2 expression and up-regulation of Bax expression induced by Almal in PC12 cells and SH-SY5Y cells. In summary, HF protects PC12 cells and SH-SY5Y cells from damage induced by Almal through reducing oxidative stress and preventing of mitochondrial-mediated apoptosis.

许多报告表明，麦芽糖酸铝（Almal）对人和动物具有潜在的毒性。我们的研究表明，Almal可以诱导PC12细胞和SH-SY5Y细胞（两种神经元细胞体外模型）的氧化损伤和细胞凋亡。Hyperforin（HF）是从贯叶连翘提取的著名抗氧化剂，消炎药，抗淀粉样蛋白和抗抑郁药提炼。在这里，我们调查了HF对培养的PC12细胞和SH-SY5Y细胞中Almal诱导的神经毒性的神经保护作用，这主要是由氧化应激引起的。在本研究中，与Almal组相比，HF显着抑制了PC12细胞中的活性氧（ROS）的形成，降低了脂质过氧化物的水平并增强了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。 SH-SY5Y细胞。此外，HF抑制了线粒体膜电位（MMP），细胞色素c的降低。（Cyt-c）释放，Alaspase在PC12细胞和SH-SY5Y细胞中诱导的caspase-3激活以及Bcl-2表达的下调和Bax表达的上调。总之，HF通过减少氧化应激和防止线粒体介导的细胞凋亡，保护PC12细胞和SH-SY5Y细胞免受Almal诱导的损害。