We performed the transformation of a wild type Chlamydomonas reinhardtii by optimizing previously developed droplet EP method. For more effective and faster optimization, we used DNA dying fluorescent molecule (Yo-Pro-1) for finding optimal EP conditions instead of using protein expression based evaluation method. By examining wider range of electrical parameter space together with the analysis of total current flow of EP process, we found optimal EP conditions. The obtained optimal EP conditions were verified by CFP transgene expression experiments. By applying the optimal EP conditions to the transformation of C. reinhardtii, we obtained transformants and analyzed them using PCR. Finally, implications and future work are discussed.

我们通过优化先前开发的液滴EP方法对野生型莱茵衣藻进行了转化。为了更有效，更快速地进行优化，我们使用DNA染色荧光分子（Yo-Pro-1）来寻找最佳的EP条件，而不是使用基于蛋白质表达的评估方法。通过检查更大范围的电参数空间以及对EP工艺总电流的分析，我们发现了最佳EP条件。通过CFP转基因表达实验验证了获得的最佳EP条件。通过将最佳EP条件应用于莱茵衣藻的转化，我们获得了转化子，并使用PCR对其进行了分析。最后，讨论了影响和未来的工作。