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Unexpected Observations: Exposure to Aromatase Inhibitor Prochloraz Did Not Alter the Vitellogenin Content of Zebrafish Ova but Did Inhibit the Growth of Larval Offspring
Environmental Science & Technology Letters ( IF 10.9 ) Pub Date : 2018-10-29 , DOI: 10.1021/acs.estlett.8b00486
Yao Dang 1 , Qian Sun 1 , Robert J. Letcher 2 , Chunsheng Liu 1, 3
Affiliation  

There is sparse information about the evaluation of the effects on ova of estrogen inhibition in fish. In this study, two exposure experiments were performed. First, adult zebrafish were exposed to aromatase inhibitor prochloraz (PCZ) (0, 3, 30, or 300 μg/L) for 21 days, and the developmental toxicity of larval offspring was evaluated. PCZ markedly decreased 17β-estradiol (E2) and vitellogenin (vtg) concentrations in plasma of females and suppressed spawned egg numbers. PCZ exposure caused bioaccumulation in ova and resulted in a significant decrease in the body length of larval offspring in 300 μg/L PCZ, but unexpectedly, it did not alter the vtg content of ova. Second, adult zebrafish were exposed to 0 or 300 μg/L PCZ for 21 days, and responses of the transcriptome and proteome in ova were characterized. Although a 21 day treatment with PCZ resulted in bioaccumulation of PCZ in ova, it was not responsible for the observed inhibition of body length growth of larval offspring as evidenced by PCZ microinjection. Furthermore, exposure of adult zebrafish to PCZ significantly downregulated mRNA expression of retinol binding protein 4 (rbp4) and changed protein concentrations related to mitochondrial energy metabolism in ova, and those changes might be responsible for the inhibition of growth of larval offspring.

中文翻译:

意外的观察:暴露于芳香酶抑制剂Prochloraz不会改变斑马鱼卵中卵黄蛋白原的含量,但会抑制幼虫后代的生长

关于评价鱼类中雌激素抑制作用对卵的影响的信息很少。在这项研究中,进行了两次暴露实验。首先,将成年斑马鱼暴露于芳香化酶抑制剂前氯唑(PCZ)(0、3、30或300μg/ L)21天,并评估幼虫后代的发育毒性。PCZ显着降低了女性血浆中的17β-雌二醇(E2)和卵黄蛋白原(vtg)浓度,并抑制了产卵数。PCZ暴露导致卵中生物积累,并导致300μg/ L PCZ中幼虫后代的体长显着减少,但出乎意料的是,它并未改变卵中vtg的含量。其次,将成年斑马鱼暴露于0或300μg/ L PCZ中21天,并表征卵子中转录组和蛋白质组的反应。尽管用PCZ治疗21天会导致卵巢中PCZ的生物蓄积,但PCZ显微注射证明,它对观察到的幼虫后代体长生长的抑制作用不负责任。此外,成年斑马鱼暴露于PCZ会显着下调视黄醇结合蛋白4的mRNA表达(rbp4)和与卵子线粒体能量代谢有关的蛋白质浓度变化,这些变化可能是抑制幼虫后代生长的原因。
更新日期:2018-10-30
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