BACKGROUND Biofilms that develop on dry surfaces in the healthcare environment have increased tolerance to disinfectants. This study compared the activity of formulated oxidizing disinfectants with products containing active ingredients against Staphylococcus aureus dry-surface biofilm (DSB) alone. METHODS DSB was grown in the CDC bioreactor with alternating cycles of hydration and dehydration. Disinfectant efficacy was tested before and after treatment with neutral detergent for 30 s, and in the presence or absence of standardized soil. Biofilms were treated for 5 min with peracetic acid (Surfex and Proxitane), hydrogen peroxide (Oxivir and 6% H2O2 solution) and chlorine (Chlorclean and sodium dichloroisocyanurate tablets). Residual biofilm viability and mass were determined by plate culture and protein assay, respectively. FINDINGS Biofilm viability was reduced by 2.8 log10 for the chlorine-based products and by 2 log10 for Proxitane, but these products failed to kill any biofilm in the presence of soil. In contrast, Surfex completely inactivated biofilm (6.3 log10 reduction in titre) in the presence of soil. H2O2 products had little effect against DSB. Biofilm mass removed in the presence and absence of soil was <30% by chlorine and approximately 65% by Surfex. Detergent treatment prior to disinfection had no effect. CONCLUSION The additives in fully formulated disinfectants can act synergistically with active ingredients, and thus increase biofilm killing whilst decreasing the adverse effect of soil. It is suggested that purchasing officers should seek efficacy testing results, and consider whether efficacy testing has been conducted in the presence of biological soil and/or biofilm.

中文翻译：

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消毒剂配方和有机土壤对氧化性消毒剂对抗生物膜的功效的影响。

背景技术在医疗环境中在干燥表面上形成的生物膜具有增加的对消毒剂的耐受性。这项研究比较了配制的氧化性消毒剂和含有活性成分的产品对单独的金黄色葡萄球菌干表面生物膜（DSB）的活性。方法DSB在CDC生物反应器中以交替的水合和脱水循环生长。在使用中性去污剂处理30 s之前和之后，以及在有无标准污垢的情况下，均测试了消毒剂的功效。生物膜用过氧乙酸（Surfex和Proxitane），过氧化氢（Oxivir和6％H2O2溶液）和氯气（Chlorclean和二氯异氰尿酸钠片剂）处理5分钟。分别通过平板培养和蛋白质测定来确定残留生物膜的生存力和质量。结果对于基于氯的产品，生物膜的生存力降低了2.8 log10，对于环戊烷，生物膜的活力降低了2 log10，但是这些产品在存在土壤时无法杀死任何生物膜。相反，Surfex在存在土壤的情况下使生物膜完全失活（滴度降低6.3 log10）。H2O2产品对DSB的影响很小。在存在和不存在土壤的情况下，去除的生物膜质量被氯<30％，被Surfex去除约65％。消毒前的去污剂处理无效。结论完全配制的消毒剂中的添加剂可以与活性成分协同作用，从而增加生物膜的杀灭力，同时减少对土壤的不利影响。建议采购人员应寻求功效测试结果，