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Enhancing carbon capture and lipid accumulation by genetic carbonic anhydrase in microalgae
Journal of the Taiwan Institute of Chemical Engineers ( IF 5.7 ) Pub Date : 2018-10-27 , DOI: 10.1016/j.jtice.2018.10.010
Way-Rong Lin , Yu-Cheng Lai , Po-Kuei Sung , Shih-I Tan , Chien-Hsiang Chang , Chun-Yen Chen , Jo-Shu Chang , I-Son Ng

Increasing atmospheric carbon dioxide (CO2) has led to a series of problems, impacting both our lives and environment. One of the solutions to capture and reduce CO2 is by carbon capture and storage (CCS). Carbonic anhydrase (CA) is an ancient enzyme existing in most organisms and it catalyzes the interconversion of CO2 and bicarbonate (HCO3). Therefore, overexpression of CA in microalgae could be a potential way to capture excess CO2 in an effective manner. In this study, an exogenous MlCA gene (i.e., 678 bp) which has been reported with highest catalytic activity was successfully overexpressed in Chlorella sorokiniana (CS) and Chlorella vulgaris (CV), respectively. The genetically engineered algae harboring exogenous CA were cultured in a specially designed Two-layer Photo Reactor (TPR) for validation. Transgenic algae with MlCA had improved biomass production, protein content and lipid accumulation. Finally, the transformants obtained a higher lipid amount up to 1.1 g/L which was 2.2-fold than that of wild types as well as accelerating the carbon capture and fixation.



中文翻译:

通过遗传碳酸酐酶提高微藻中的碳捕获和脂质积累

大气中二氧化碳(CO 2)的增加导致了一系列问题,影响了我们的生活和环境。捕获和减少CO 2的解决方案之一是通过碳捕获和存储(CCS)。碳酸酐酶(CA)是存在于大多数生物体一个古老的酶和其催化CO的相互2和碳酸氢盐(HCO 3 - )。因此,微藻中CA的过表达可能是有效捕获过量CO 2的潜在方法。在这项研究中,已报道了具有最高催化活性的外源性MlCA基因(678 bp)在Sorokiniana小球藻(CS)和普通小球藻(CV)。将带有外源CA的基因工程藻类培养在专门设计的两层光反应器(TPR)中进行验证。带有MlCA的转基因藻类改善了生物量的产生,蛋白质含量和脂质积累。最后,转化子获得了高达1.1 g / L的更高脂质量,是野生型脂质的2.2倍,并加速了碳的捕获和固定。

更新日期:2018-10-27
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