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Ferrochelatase activity of plant frataxin
Biochimie ( IF 3.9 ) Pub Date : 2018-10-18 , DOI: 10.1016/j.biochi.2018.10.009
Alejandro M. Armas , Manuel Balparda , Agustina Terenzi , Maria V. Busi , Maria A. Pagani , Diego F. Gomez-Casati

Frataxin plays a key role in cellular iron homeostasis of different organisms. It is engaged in several activities at the FeS cluster assembly machinery and it is also involved in heme biosynthesis. In plants, two genes encoding ferrochelatases (FC1 and FC2) catalyze the incorporation of iron into protoporphyrin IX in the last stage of heme synthesis in chloroplasts. Despite ferrochelatases are absent from other cell compartments, a remaining ferrochelatase activity has been observed in plant mitochondria. Here we analyze the possibility that frataxin acts as the iron donor to protoporphyrin IX for the synthesis of heme groups in plant mitochondria. Our findings show that frataxin catalyzes the formation of heme in vitro when it is incubated with iron and protoporphyrin IX. When frataxin is combined with AtNFS1 and AtISD11 the ferrochelatse activity is increased. These results suggest that frataxin could be the iron donor in the final step of heme synthesis in plant mitochondria, and constitutes an important advance in the elucidation of the mechanisms of heme synthesis in plants.



中文翻译:

植物frataxin的铁螯合酶活性

Frataxin在不同生物的细胞铁稳态中起关键作用。它在Fe S团簇组装机器上从事多项活动,还参与血红素的生物合成。在植物中,编码铁螯合酶的两个基因(FC1FC2)在叶绿体中血红素合成的最后阶段催化铁掺入原卟啉IX中。尽管其他细胞区隔中不存在亚铁螯合酶,但在植物线粒体中仍观察到了剩余的亚铁螯合酶活性。在这里,我们分析了frataxin充当原卟啉IX的铁供体在植物线粒体中合成血红素基团的可能性。我们的发现表明,frataxin在体外催化血红素的形成与铁和原卟啉IX一起孵育时。当frataxin与AtNFS1和AtISD11组合时,铁螯合酶活性增加。这些结果表明,frataxin可能是植物线粒体中血红素合成的最终步骤中的铁供体,并且在阐明植物中血红素合成的机制方面具有重要的进展。

更新日期:2018-10-18
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