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Zwitterionic poly(carboxybetaine) functionalized conducting polymer polyaniline nanowires for the electrochemical detection of carcinoembryonic antigen in undiluted blood serum
Bioelectrochemistry ( IF 5 ) Pub Date : 2018-10-11 , DOI: 10.1016/j.bioelechem.2018.09.006
Jiasheng Wang , Ni Hui

Biocompatible materials, such as zwitterionic poly(carboxybetaine methacrylate) (polyCBMA), are of extraordinary importance in growth of bioelectronics and biosensors, because they not only greatly suppress nonspecific protein adsorption, but also have rich functional groups to facilitate the fixation of biological molecules. A novel nanocomposite was synthesized herein through modification polyCBMA onto conducting polymer polyaniline (PANI) nanowire surface. The prepared polyCBMA/PANI composite, integrating the good conductivity of PANI nanowires with the excellent antifouling capability of polyCBMA, provided a wonderful matrix for the growth of ultrasensitive and low fouling biosensor. Carcinoembryonic antigen (CEA), an important biomarker for a variety of cancers, was utilized as a model test. Furthermore its antibody was fixed onto polyCBMA/PANI for the preparation of CEA biosensor. DPV was applied as a sensing principle with information at which peak potential and current the signals were recorded. The peak currents were in inverse proportion to the logarithm of CEA concentration in the range from 1.0 × 10−14 g mL−1 to 1.0 × 10−10 g mL−1, with a detect limit of 3.05 fg mL−1. Furthermore, this low fouling, label-free biosensor has been utilized for assaying in undiluted human serum samples with resisting serious nonspecific protein adsorption, demonstrating its feasible potential application in clinical analysis of CEA.



中文翻译:

两性离子聚(羧基甜菜碱)功能化的导电聚合物聚苯胺纳米线用于电化学检测未稀释血清中的癌胚抗原

生物相容性材料,例如两性离子聚甲基丙烯酸甜菜碱酯(polyCBMA),在生物电子学和生物传感器的发展中具有极其重要的意义,因为它们不仅极大地抑制了非特异性蛋白质的吸附,而且还具有丰富的官能团以促进生物分子的固定。本文通过修饰聚CBMA到导电聚合物聚苯胺(PANI)纳米线表面上合成了新型纳米复合材料。所制备的polyCBMA / PANI复合材料结合了PANI纳米线的良好导电性和polyCBMA的优异防污性能,为超灵敏和低污损生物传感器的生长提供了绝佳的基质。癌胚抗原(CEA)是多种癌症的重要生物标志物,被用作模型测试。此外,将其抗体固定在polyCBMA / PANI上,用于制备CEA生物传感器。将DPV用作感测原理,并带有记录信号峰值电势和电流的信息。峰值电流与CEA浓度的对数成反比,范围为1.0×10-14  g mL -1至1.0×10 -10  g mL -1,检出限为3.05 fg mL -1。此外,这种低结垢,无标记的生物传感器已被用于在未稀释的人血清样品中进行测定,具有抗严重的非特异性蛋白质吸附的能力,证明了其在CEA临床分析中的潜在应用价值。

更新日期:2018-10-11
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