The allelopathic effects of extracts isolated from the culture filtrate of diatom Phaeodactylum triconutum Bohlin on typical marine microalgae Prorocentrum donghaiense Lu and Dunaliella salina Teodoresco were investigated by determining different physiological and biochemical parameters, such as growth rate, membrane systems and esterase activity under controlled laboratory conditions. The growth of P. donghaiense was significantly inhibited immediately after exposure to the allelochemicals, while the algae density of D. salina was less sensitive. Chlorophyll-a content, membrane systems, as well as esterase activity were simultaneously investigated by flow cytometry with particular fluorescent markers and exhibited changeable sensitivities. The results demonstrated that the membrane systems of P. donghaiense were suppressed by the allelochemicals directly, causing loss of integrity and membrane penetration. Esterase activity was the most sensitive indicator as that of P. donghaiense cells significantly increased in short time and was inhibited subsequently. However, the membrane of D. salina remained intact still after exposure to the extracts and the esterase activity was only inhibited on last day during experiment period. Membrane potential and chlorophyll-a content of the two marine algae also showed somewhat different changes, as that of P. donghaiense cells were impaired after 5 day exposure to all volume conditions while these two characteristics of D. salina was only suppressed by exposure to high volume of the allelochemicals on day 6. The present results indicated that the inhibition of culture filtrate of P. triconutum on P. donghaiense was algicidal whereas the effect on D. salina appeared to be algistatic.

通过在控制实验室条件下测定生长速率，膜系统和酯酶活性等不同的生理和生化参数，研究了从硅藻三角藻波氏菌培养物滤液中提取的提取物对典型海洋微藻Prorocentrum donghaiense Lu和Dunaliella salina Teodoresco的化感作用。。暴露于化感物质中后，P。donghaiense的生长受到显着抑制，而盐藻D. salina的藻类密度不太敏感。叶绿素a含量，膜系统，以及酯酶活性同时通过流式细胞仪与特定的荧光标记进行了调查，并显示出可变的敏感性。结果表明，化感物质直接抑制东海假单胞菌的膜系统，导致完整性和膜穿透性的损失。酯酶活性是最敏感的指标，因为东海假单胞菌的细胞活性在短时间内显着增加，随后被抑制。然而，盐藻D. salina的膜暴露于提取物后仍保持完整，酯酶活性仅在实验期间的最后一天被抑制。两种海藻的膜电位和叶绿素-a含量也显示出一些不同的变化，因为暴露于所有体积条件下5天后，东海疫杆菌的细胞膜受损，而盐藻的这两个特征仅通过暴露于高浓度下而受到抑制。在第6天的化感物质的总体积中。本结果表明，P。triconutum的培养物滤液对东海疟原虫的抑制作用是杀藻的，而对D. salina的抑制作用似乎是止痛的。