Present study was designed to evaluate the adverse effect of glyphosate-based herbicide, Excel Mera 71 in Anabas testudineus on comparative basis under field and laboratory conditions. Field (750 g/acre) and laboratory (17.2 mg/L) experiments were performed for a period of 30 days. For field experiment special type of cages were prepared. Fish gill, liver, and kidney were analyzed for histology and ultrastructural responses. A significant increment in morphometric indices (DTC) was observed in gill, liver and kidney of A. testudineus under laboratory condition (p < 0.05) and responses showed the degree of pathogenicity in the order of liver > kidney > gills. However, under field study significant increase in DTC value was observed in gill and liver (p < 0.05). Among the scanning electron microscopic (SEM) observations necrosis and loss of microridges, and damage in stratified epithelial cells were prominent in gill, although higher prevalence of alterations was observed under laboratory study than field study. Additionally, transmission electron microscopic (TEM) observations also depicted higher prevalence of pathological lesions under laboratory study compared with field observation. Among the TEM observations damage in chloride and pavement cells, degenerative mitochondria and nucleus (in gill); severe vacuolation, necrosed nucleus and vesiculated network in case of liver and degenerated epithelial cells, cytoplasmic vacuolation, and damage in proximal convoluted tubules (PCT) in case of kidney were prominent. Therefore, these findings demonstrated that Excel Mera 71 induces significant damage in tissues of A. testudineus and these responses might be considered as biomarkers for monitoring herbicidal toxicity on fish in aquatic body.

本研究旨在在野外和实验室条件下，比较草甘膦基除草剂Excel Mera 71对Anabas testudineus的不良影响。进行了30天的田间试验（750 g /英亩）和实验室试验（17.2 mg / L）。为了进行现场实验，准备了特殊类型的笼子。分析鱼g，肝和肾的组织学和超微结构反应。在实验室条件下，在睾丸曲霉的腮，肝和肾中观察到形态计量指数（DTC）的显着增加（p  <0.05），反应显示出致病性的程度依次为肝>肾> ill。然而，在实地研究中，g和肝脏中DTC值显着增加（p <0.05）。在扫描电子显微镜（SEM）的观察结果中，ill中坏死和微脊的丢失以及分层上皮细胞的损伤非常明显，尽管在实验室研究中观察到的改变发生率高于现场研究。此外，与现场观察相比，在实验室研究中，透射电子显微镜（TEM）观察还显示出更高的病理病变患病率。在透射电镜观察中，对氯化物和路面细胞，变性线粒体和细胞核（g）的损害；严重的空泡，肝坏死的核和囊状网络以及变性的上皮细胞，胞浆的空泡和肾脏的近端曲折小管（PCT）的损伤非常明显。所以，睾丸曲霉和这些反应可被认为是监测水生鱼类对鱼的除草毒性的生物标记。