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Genome sequence and comparative genomics of Rhizobium sp. Td3, a novel plant growth promoting phosphate solubilizing Cajanus cajan symbiont
Microbiological Research ( IF 6.7 ) Pub Date : 2018-09-24 , DOI: 10.1016/j.micres.2018.09.007
Bhagya Iyer , Shalini Rajkumar

Rhizobium sp. Td3 is a Sesbania plant growth promoting, Cajanus cajan nodulating rhizobia. Studying its whole genome was important as it is a potent phosphate solubilizer with constitutive gluconic acid production ability through operation of the periplasmic glucose oxidation pathway even under conditions of catabolite repression. This is in contrast to the other explored phosphate solubilizers. Rhizobial isolates sequenced so far are known to lack components of the direct glucose oxidation pathway and cannot produce gluconic acid on its own. Here, we present the genome sequence of Rhizobium sp. Td3. Genome comprises of a single chromosome of size 5,606,547 bp (5.6 Mb) with no symbiotic plasmid. Rhizobium leguminosarum bv. viciae USDA2370 was the closest whole genome known. 109 genes responsible for diverse plant growth promoting activities like P solubilization, synthesis of acetoin, nitric oxide, indole-3 acetic acid, exopolysaccharide, siderophore and trehalose have been identified. Flagellar proteins, genes encoding antibiotic and metal resistance, enzymes required for combating oxidative stress as well as attachment and colonization in the plant rhizosphere are also present. Availability of genome sequence of such a versatile plant growth promoting agent will help in exploiting all the phyto-beneficial traits of Td3 for its use as a biofertilizer.



中文翻译:

根瘤菌的基因组序列和比较基因组学Td3,一种新型植物生长促进磷酸盐溶解Cajanus cajan共生体

根瘤菌 Td3是促进塞斯巴尼亚植物生长的根瘤菌根瘤菌(Cajanus cajan)。研究其整个基因组非常重要,因为它是一种有效的磷酸盐增溶剂,即使在分解代谢物抑制的条件下,通过周质葡萄糖氧化途径的操作,它也具有组成型葡糖酸生产能力。这与其他探索的磷酸盐增溶剂相反。迄今为止,已知测序的根瘤菌分离物缺少直接葡萄糖氧化途径的成分,并且不能自行产生葡萄糖酸。在这里,我们介绍了根瘤菌的基因组序列Td3。基因组由一个没有共生质粒的大小为5,606,547 bp(5.6 Mb)的染色体组成。豆科根瘤菌bv。蚕豆USDA2370是已知的最接近的完整基因组。已经鉴定出109种基因,这些基因负责多种植物生长促进活性,例如P增溶,乙酰丙酮,一氧化氮,吲哚-3乙酸,胞外多糖,铁载体和海藻糖的合成。还存在鞭毛蛋白,编码抗生素和金属抗性的基因,抵抗氧化胁迫以及植物根际中的附着和定植所需的酶。这种多功能植物生长促进剂的基因组序列的可用性将有助于利用Td3的所有植物有益性状作为生物肥料。

更新日期:2018-09-25
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