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Identifying and characterizing functional 3’ nucleotide addition in the miRNA pathway
Methods ( IF 4.8 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.ymeth.2018.08.006
A Maxwell Burroughs 1 , Yoshinari Ando 2
Affiliation  

Over the past decade, modifications to microRNAs (miRNAs) via 3' end nucleotide addition have gone from a deep-sequencing curiosity to experimentally confirmed drivers of a range of regulatory activities. Here we overview the methods that have been deployed by researchers seeking to untangle these diverse functional roles and include characterizing not only the nucleotidyl transferases catalyzing the additions but also the nucleotides being added, and the timing of their addition during the miRNA pathway. These methods and their further development are key to clarifying the diverse and sometimes contradictory functional findings presently attributed to these nucleotide additions.

中文翻译:

识别和表征 miRNA 途径中的功能性 3' 核苷酸添加

在过去的十年中,通过 3' 末端核苷酸添加对 microRNA (miRNA) 的修饰已经从深度测序的好奇心转变为经过实验证实的一系列监管活动的驱动因素。在这里,我们概述了寻求解开这些不同功能作用的研究人员已经部署的方法,包括不仅表征催化添加的核苷酸转移酶,还表征添加的核苷酸,以及它们在 miRNA 途径中添加的时间。这些方法及其进一步发展是澄清目前归因于这些核苷酸添加的多样化且有时相互矛盾的功能发现的关键。
更新日期:2019-01-01
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