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Isolation and characterization of actinomycetes from Mural paintings of Snu- Sert-Ankh tomb, their antimicrobial activity, and their biodeterioration
Microbiological Research ( IF 6.7 ) Pub Date : 2018-08-15 , DOI: 10.1016/j.micres.2018.08.005
Abeer F. Elhagrassy

A total of 35 actinomycetes were isolated from the surface of mural paintings of snu- sort- ankh in El Leasht, Egypt during four seasons all over 2016-2017. In 2009 this tomb was deteriorated by "Aspergillus niger, A. flavus, Fusarium moniliforme, Alternaria alternate, Rhizopus stolonifera, Bacillus subtilis, Bacillus cereus, and micrococcus iuteus." In 2017 the isolation of swabs presents only Aspergillus niger and about 35 actinomycetes classified to three different groups "Streptomyces, Nocardia, and Micromonospora" only five species belong to Streptomyces group showed antimicrobial activity against the previous microorganisms. These actinomycetes were identified according to their sequences in the GenBank to "Streptomyces spectabilis, S. alborgriseolus, S. globsus, S. corchorstt, S. ambofactens." In the other hand, the pigments of the wall paintings of the tomb (Egyptian blue, Egyptian green, Goethite) that analyzed by SEM-EDX, FTIR were measured by the spectrophotometer in both 2009 and 2017. The results showed that the actinomycetes could produce extracellular pigments causing a color change of archaeological pigments otherwise it helps in inhibition the growth of the previous microorganisms found in 2009. The optimization factors for increasing the antibiotic production of the Streptomyces were 3% NaCl and temperature between 30:35°c in Alkin pH (pH = 7.5).



中文翻译:

从Snu-Sert-Ankh墓的壁画中分离和表征放线菌,它们的抗菌活性和生物降解

在2016-2017年的四个季节中,从埃及El Leasht的snusort-ankh壁画壁画表面总共分离出35株放线菌。2009年,该墓因“黑曲霉,黄曲霉,镰刀镰刀菌,备用链霉菌,根腐菌,枯草芽孢杆菌,蜡状芽孢杆菌和小球菌”而恶化。在2017年,棉签的分离仅显示黑曲霉和大约35种放线菌,分为三个不同的类别“链霉菌,诺卡氏菌和微单孢菌”,属于链霉菌组的只有5种对先前的微生物表现出抗菌活性。这些放线菌是根据它们在GenBank中的序列进行鉴定的,以“链霉菌(Streptomyces spectabilis),S。alborgriseolus,S。globsus,S。corchorstt,S。ambofactens。”另一方面,通过SEM-EDX分析的墓室壁画颜料(埃及蓝,埃及绿,针铁矿),用分光光度计在2009年和2017年对FTIR进行了测量。结果表明,放线菌可能产生细胞外色素,引起考古色素的颜色变化,否则有助于抑制2009年发现的先前微生物的生长。链霉菌的抗生素产生量为3%NaCl,Alkin pH(pH = 7.5)中温度在30:35°c之间。

更新日期:2018-08-15
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