We previously reported that ATF3 and Runx2 are involved in breast cancer progression and bone metastasis. The expression of these genes can be controlled by post-transcriptional regulators such as microRNAs (miRNAs). In this study, we identified and validated the functional role of miR-590–3p in human breast cancer cells (MDA-MB231). There was an inverse correlation between the expression of miR-590–3p and its putative target genes, ATF3 and Runx2 in these cells. Overexpression of miR-590–3p decreased the expression of ATF3 and Runx2 at the mRNA and protein levels in MDA-MB231 cells. Luciferase reporter assay identified a direct interaction of 3’ UTRs of ATF3 and Runx2 with miR-590–3p in these cells. Overexpression of miR-590–3p also decreased proliferation and increased apoptosis of breast cancer cells. Based on our results, we suggest that miR-590–3p might have potential clinical applications towards controlling breast cancer progression and bone metastasis.

我们先前曾报道ATF3和Runx2与乳腺癌的进展和骨转移有关。这些基因的表达可以通过转录后调控因子如microRNA（miRNA）来控制。在这项研究中，我们确定并验证了miR-590-3p在人乳腺癌细胞（MDA-MB231）中的功能作用。在这些细胞中，miR-590-3p的表达与其假定的靶基因ATF3和Runx2之间呈负相关。在MDA-MB231细胞的mRNA和蛋白质水平上，miR-590-3p的过表达降低了ATF3和Runx2的表达。萤光素酶报告基因检测鉴定了这些细胞中ATF3和Runx2的3'UTR与miR-590-3p的直接相互作用。miR-590-3p的过表达也降低了乳腺癌细胞的增殖和凋亡。根据我们的结果，