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Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin
Studies in Mycology ( IF 16.5 ) Pub Date : 2018-07-05 , DOI: 10.1016/j.simyco.2018.06.003
N. Maryani , L. Lombard , Y.S. Poerba , S. Subandiyah , P.W. Crous , G.H.J. Kema

Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt or Panama disease on banana, is one of the major constraints in banana production worldwide. Indonesia is the centre of origin for wild and cultivated bananas, which likely co-evolved with Foc. This study explored the widest possible genetic diversity of Foc by sampling across Indonesia at 34 geographically and environmentally different locations in 15 provinces at six islands. This resulted in a comprehensive collection of ∼200 isolates from 40 different local banana varieties. Isolates were identified and assessed using sequence analysis of the translation elongation factor-1alpha (tef1), the RNA polymerase II largest subunit (rpb1), and the RNA polymerase II second largest subunit (rpb2). Phylogenetic analyses of these genes allowed the identification of 180 isolates of Fusarium oxysporum f. sp. cubense (Foc), and 20 isolates of the Fusarium fujikuroi species complex (FFSC), the Fusarium incarnatum-equiseti species complex (FIESC), and the Fusarium sambucinum species complex (FSSC). Further analyses, incorporating a worldwide collection of Foc strains, revealed nine independent genetic lineages for Foc, and one novel clade in the Fusarium oxysporum species complex (FOSC). Selected isolates from each lineage were tested on the banana varieties Gros Michel and Cavendish to characterise their pathogenicity profiles. More than 65 % of the isolates were diagnosed as Tropical Race 4 (Foc-TR4) due to their pathogenicity to Cavendish banana, which supports the hypothesis that Foc-TR4 is of Indonesian origin. Nine independent genetic lineages for Foc are formally described in this study. This biodiversity has not been studied since the initial description of Foc in 1919. This study provides a detailed overview of the complexity of Fusarium wilt on banana and its diversity and distribution across Indonesia.



中文翻译:

香蕉枯萎病原镰刀菌的系统发育和遗传多样性。sp。印尼起源中心的立方

尖孢镰刀菌f。sp。香蕉枯萎病(FOC),枯萎病或巴拿马病对香蕉的致病因子,是在香蕉生产全世界的主要制约因素之一。印度尼西亚是野生香蕉和栽培香蕉的原产地,很可能与Foc共同进化。这项研究通过在六个岛屿的15个省的34个地理和环境不同的地点对整个印度尼西亚进行采样,探索了Foc可能的最大遗传多样性。结果是从40个不同的本地香蕉品种中收集了约200个分离株。分离物的鉴定和评估使用了RNA聚合酶II最大亚基(rpb1)的翻译延伸因子1alpha(tef1)的序列分析。),以及RNA聚合酶II的第二大亚基(rpb2)。这些基因的系统发育分析可以鉴定镰刀菌f的180个分离株。sp。香蕉枯萎病(FOC),和20株镰孢苗病菌物种络合物(FFSC)时,镰孢incarnatum-木贼物种络合物(FIESC)和镰孢接骨木物种络合物(FSSC)。进一步的分析,包括全球范围内收集的Foc菌株,揭示了Foc的9个独立遗传谱系,以及尖孢镰刀菌中的一个新进化枝物种复合体(FOSC)。在香蕉品种Gros Michel和Cavendish上测试了每个谱系的分离株,以鉴定其致病性。由于它们对卡文迪许香蕉具有致病性,因此超过65%的分离物被诊断为热带种族4(Foc-TR4),这支持了Foc-TR4起源于印度尼西亚的假设。这项研究正式描述了Foc的九个独立遗传谱系。自1919年首次对Foc进行描述以来,尚未对这种生物多样性进行过研究。该研究提供了香蕉枯萎病的复杂性及其在印度尼西亚的多样性和分布的详细概述。

更新日期:2018-07-05
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