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  • 更新日期:2019-01-18
  • Hypouricemic and nephroprotective roles of anthocyanins in hyperuricemic mice.
    Food Funct. (IF 3.289) Pub Date : 2019-01-18
    Xiaoyun Qian, Xu Wang, Jing Luo, Yao Liu, Juan Pang, Hanyue Zhang, Zhongliang Xu, Jiewen Xie, Xinwei Jiang, W. Ling

    Abstract Hyperuricemia (HUA) is a universal metabolic disorder characterized by a high level of uric acid in the serum. Anthocyanins (ACNs) are a group of natural flavonoids that have shown favourable bioactivities on metabolic syndrome but the effect on uric acid metabolism remain underexplored. The present study investigated the hypouricemic effects of ACNs in a mice model and further studied the potential mechanisms. ICR mice based on a high-yeast diet, were administered potassium oxonate (PO, 280 mg/kg body weight) and inosine (400 mg/kg body weight) to induce a hyperuricemia model, meanwhile, ACNs were supplemented by gavage. Mice were sacrificed after 3 weeks treatment. ACNs administration significantly reduced serum uric acid (SUA), blood urea nitrogen (BUN) and serum creatinine (Scr) levels and suppressed xanthine oxidase (XOD) activity in mice serum and liver. In addition, ACNs down-regulated the expression of hepatic XOD, caspase-1, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) and regulated the expression of renal urate transporters URAT1, GLUT9, ABCG2, OAT1, OAT3, OCT1, OCT2, OCTN1 and OCTN2. According to histological analysis, ACNs treatment exhibited hepatoprotective and nephroprotective effects in hyperuricemic mice. In conclusion, ACNs reduced urate production and promoted the uric acid excretion from renal, which suggests the potential of ACNs for the future treatment of HUA.

    更新日期:2019-01-18
  • Extrusion of apple pomace increases antioxidant activity upon in vitro digestion
    Food Funct. (IF 3.289) Pub Date : 2019-01-18
    Guo Liu, DanYang Ying, Baoyan Guo, Lijiang Cheng, Bruce May, Anthony Bird, Luz Sanguansri, Yong Cao, Maryann Augustin

    Apple pomace, a by-product of juice production, is a high-fibre, high-polyphenol functional food ingredient. Extrusion (barrel moisture 15%, 20% or 30%) of apple pomace, followed by drying, allows it to be supplied in a convenient form. Extrusion caused degradation of the apple pomace cell wall structure. Water solubility was significantly increased by extrusion but oil holding capacity was reduced. Total extractable polyphenols, measured as gallic acid equivalents, were reduced by extrusion (barrel moisture 30%) but was not affected by extrusion at lower barrel moistures (15% or 20%). However, individual sub-groups of extractable flavanols, flavonols, phenolic acids and dihydrochalcones were increased by extrusion. There was little effect of extrusion on the release of total polyphenols from the matrix into the supernatant, as measured by total extractable polyphenols (measured as gallic acid equivalents) released during in vitro digestion. There were marked increase in total flavanols, phenolic acids and dihydrochalones released into the supernatant during the gastric phase but changes in flavonoids were less obvious. The changes in bioaccessibility of individual polyphenols released during intestinal digestion was dependent on the type of polyphenol and extrusion conditions. The antioxidant activity, as measured using oxygen radical absorbance capacity (ORAC) of the bioaccessible nutrients released upon in vitro intestinal digestion, was significantly enhanced by extrusion (from 78.2 to 400-500 µmol Trolox equivalents/mL at ileal phase). The increased ORAC may be attributed in part to the increased release of individual polyphenols.

    更新日期:2019-01-18
  • Coenzyme Q10 attenuates high-fat diet-induced non-alcoholic fatty liver disease through activation of AMPK pathway
    Food Funct. (IF 3.289) Pub Date : 2019-01-18
    Ke Chen, Xu Chen, Hongliang Xue, Zhang Peiwen, Wanjun Fang, Xuechen Chen, W. Ling

    Coenzyme Q10 (CoQ10) is a well-known anti-adipogenic factor that possesses the capability to regulate non-alcoholic fatty liver disease (NAFLD). However, the mechanism by which CoQ10 acts on NAFLD is still unclear. In this study, the role of CoQ10 in the prevention of NAFLD was investigated in vivo and in vitro. C57BL/6J mice were fed with a normal diet, high-fat diet (HFD) or HFD supplemented with CoQ10 (1800 mg/kg HFD) for 24 weeks. HepG2 cells were treated with sodium palmitate for investigating the mechanism of the action of CoQ10 on NAFLD. The results showed that CoQ10 alleviated HFD-induced weight gain and NAFLD, accompanied by an anti-hyperlipidaemia effect, by reducing the serum triglycerides, total cholesterol, and low-density lipoprotein cholesterol levels. Importantly, CoQ10 could downregulate the expression of sterol regulatory element-binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS), which are related to the lipid synthesis, and upregulate the expression of peroxisome proliferator-activated receptors α(PPARα) and carnitine palmitoyltransferase1 (CPT-1) associated with fatty acid oxidation. Similar to the results from mice, treatment with CoQ10 alleviated the sodium palmitate-induced hepatocyte steatosis via inhibition of lipogenesis and promotion of fatty acid oxidation. However, Compound C, as the AMPK inhibitor, can significantly block the benefits derived from CoQ10 treatment. In conclusion, CoQ10 could serve as an AMPK activator and regulate the hepatic lipid metabolism to inhibit the abnormal accumulation of hepatic lipids and prevent NAFLD progression.

    更新日期:2019-01-18
  • Structural identification and osteogenic activity of a novel heteropolysaccharide obtained from female flowers of Humulus lupulus
    Food Funct. (IF 3.289) Pub Date : 2019-01-17
    Xiaoxia Chen, Xin Hou, Degang Qing, Qian Zhang, Chunyan Yan

    Humulus lupulus is distributed mainly in Asia, Europe, and North America, and the flowers and infructescences are used worldwide in beer brewing as an essential flavoring ingredient. In China, the main producing areas are the Xinjiang Uygur Autonomous Region and Gansu provinces. The annual production of H. lupulus in China accounts for about 13% of worldwide production, ranking third after the United States and Germany. The female flower of H. lupulus also is used in traditional Chinese medicine, but the bioactive components responsible for its benefits remain unclear. In this study, we isolated a homogeneous polysaccharide (HLP50-2) from female flowers of H. lupulus via DEAE-52 cellulose and Sephadex G-75 gel filtration chromatography. The molecular weight was 73.25 kDa. Chemical methods, gas chromatography-mass spectrometry, and nuclear magnetic resonance analyses showed that HLP50-2 consisted of →4)-β-D-GlcAp-(1→, →4)-α-L-Rhap-(1→, →5)-α-L-Araf-(1→, α-L-Araf-(1→, →3,6)-β-D-Galp-(1→, α-D-Galp-(1→, →3,4,6)-β-D-Galp-(1→, β-D-Xylp-(1→, →6)-α-D-Galp-(1→, and →4)-β-D-Galp-(1→. Advanced structural analysis indicated that HLP50-2 has a sheet-like structure with uneven distribution and the surface was smooth. In addition, we used MC3T3-E1 cells to evaluate the osteogenic activity of HLP50-2. The results show that HLP50-2 significantly promoted the proliferation, differentiation, and mineralization of MC3T3-E1 cells at concentrations of 0.17 μM and 0.34 μM. In particular, at 0.34 μM, the promoting mineralization effect of HLP50-2 was better than that of the positive control, 17β-estradiol, and the alkaline phosphatase activity after 10 d of treatment was comparable to that of the positive control. The extraction, isolation, purification, structural identification, and osteogenic activity of H. lupulus polysaccharides suggest that homogeneous polysaccharide HLP50-2 has pronounced anti-osteoporosis activity. Therefore, it has broad application prospects in pharmaceutical industries and health-care products.

    更新日期:2019-01-18
  • Identification and availability of peptides from lactoferrin in gastrointestinal tract of mice
    Food Funct. (IF 3.289) Pub Date : 2019-01-17
    Fengjiao Fan, Pujie Shi, Hui Chen, Maolin Tu, Zhenyu Wang, Weihong Lu, Ming Du

    Lactoferrin is a protein with multifunctional bioactivities. However, limited studies have reported the digestion behavior of lactoferrin in mice. In the present study, the metabolic fate of lactoferrin in the gastrointestinal tract (GIT) of mice was investigated. The mice were orally administered 4 mg of lactoferrin. The contents in stomach, duodenum, jejunum and ileum were collected at 0.5 h, 1 h, 2 h and 3 h after administration. The peptides in contents of stomach, duodenum, jejunum and ileum were identified by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) coupled with capillary electrophoresis (CE). At 0.5 h after administration, 350, 47, 118 and 26 novel peptides were identified in contents of stomach, duodenum, jejunum and ileum, respectively. At 1 h after administration, 175, 23, 3 and 8 novel peptides were identified in contents of stomach, duodenum, jejunum and ileum, respectively. At 2 h and 3 h after administration, 96 and 2 novel peptides were identified in contents of stomach, respectively. Among these peptides, 10 peptides were generated from gastrointestinal digestions and could play physiological roles when they were absorbed through intestinal in its original form. This work provided some theoretical basis for clarifying the bioactive mechanisms of lactoferrin.

    更新日期:2019-01-17
  • Extrinsic wheat fibre consumption enhances faecal bulk and stool frequency; a randomized controlled trial
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Nicole de Wit, Diederik Esser, Els Siebelink, Anne Fischer, Juergen Sieg, Jurriaan Mes
    更新日期:2019-01-17
  • As good as expected? How consumer expectations and addition of vegetable pieces to soups influence sensory perception and liking
    Food Funct. (IF 3.289) Pub Date : 2019-01-08
    Marco Santagiuliana, Irene A. F. van den Hoek, Markus Stieger, Elke Scholten, Betina Piqueras-Fiszman
    更新日期:2019-01-17
  • 更新日期:2019-01-17
  • Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata ‘Chachi’)
    Food Funct. (IF 3.289) Pub Date : 2019-01-16
    Manqin Fu, Bo Zou, Kejing An, Yuanshan Yu, Daobang Tang, Jijun Wu, Yujuan Xu, Huihui Ti

    Pericarpium Citri Reticulatae (PCR, Citrus reticulata ‘Chachi,’ Guangchenpi in Chinese), is one of the most famous Chinese citrus herbal medicines. The in vivo anti-asthmatic activity of ‘Chachi’ PCR was investigated using a histamine-induced experimental asthma model in guinea pigs. Two alkaloid-type compounds, synephrine and stachydrine, were analyzed and identified from the ‘Chachi’ PCR alkaloid fraction. The alkaloid fraction and synephrine protected against histamine-induced experimental asthma in guinea pigs in a dose-dependent manner. A high, middle, and low dose of ‘Chachi’ PCR alkaloid fraction significantly increased the specific airway resistance by 284%, 328%, and 355%, respectively, and decreased dynamic compliance by 57%, 67%, and 75%, respectively. A similar change was observed for synephrine. The expression of eosinophils in bronchoalveolar lavage fluid (BALF) and IgE, IL-4, and IL-5 in serum of histamine-induced experimental asthmatic guinea pigs were significantly downregulated by the ‘Chachi’ PCR alkaloid fraction and synephrine compared to the control group, whereas stachydrine did not have a statistically significant effect on the expression of tested inflammatory cells (leucocyte, eosinophils, neutrophils, and lymphocytes), immunoglobulin (IgE), or cytokines (IL-4 and IL-5). The pathological changes in lung tissues showed that the infiltration of inflammatory cells around the bronchia was observed in each treatment group

    更新日期:2019-01-17
  • Comparison on the nutritional properties and biological activities of kiwifruit (Actinidia) and their different forms products: How to make kiwifruit more nutritious and functional
    Food Funct. (IF 3.289) Pub Date : 2019-01-16
    Tingting Ma, Tian Lan, Yanlun Ju, Guo Cheng, Zhiluo Que, Tonghui Geng, Yulin Fang, Xiangyu Sun

    The nutritional properties and biological activities of kiwifruit and their different products made from same raw kiwifruit were investigated. Compared with Hayward, three new kiwifruit varieties Qinmei, Hongyang and Huayou showed better nutritional properties and biological activities. After processing into different products, the nutritional properties and biological activities of kiwifruit changed substantially but still showed a correlation with variety characteristics. Processing kiwifruit into juice, wine and vinegar retained a higher Vc and polyphenol than dried slices and jam and demonstrated better biological activities, while dried slices and jam provided more mineral elements than the three liquid products. In addition, the fermentation products wine and vinegar showed similar nutritional composition that were present at higher concentration than in juice, which indicated that fermentation helps in the dissolution of nutrient substances, while the thermal processing products dried slices and jam showed similar nutritional composition. Consuming kiwifruit supplies more comprehensive nutritional composition than any kiwifruit products.

    更新日期:2019-01-16
  • Oleuropein inhibits esophageal cancer through hypoxic suppression of BTG3 mRNA
    Food Funct. (IF 3.289) Pub Date : 2019-01-16
    Fengli Zhang , Mei Zhang

    Esophageal cancer (EC) is among the severest cancers causing the most fatalities around the world with increasing incidences. Oleuropein exhibits anti-tumor property in several human cancers. We aimed to investigate the effect of oleuropein on human EC, and to reveal the molecular target involved in EC tumorigenesis. Cell proliferation, migration and invasion assays were performed to assess the effect of oleuropein on EC cells. Xenograft tumor mouse model was utilized to assess the in vivo effect of oleuropein. Hypoxia-inducible factor-1α (HIF1α) and B-cell translocation gene 3 (BTG3) expressions were examined in oleuropein-treated EC cells. Regulatory role of HIF1α on BTG3 mRNA was evaluated by chromatin immunoprecipitation and luciferase reporter assays. Oleuropein inhibited growth of EC cell and xenograft EC tumor, as well as inhibited HIF1α and upregulated BTG3 expressions. BTG3 mRNA expression was under hypoxia inhibition through the HRE in its promoter region. BTG3 knockdown abolished inhibitory effect of oleuropein on EC cell in vitro, as well as on EC xenograft tumor in vivo. Oleuropein inhibits EC tumorigenesis through hypoxic suppression of BTG3 mRNA, supporting the clinical application of oleuropein, and HIF1α and BTG3 mRNA as potential molecular targets in treatments against EC.

    更新日期:2019-01-16
  • Effect of catechin liposomes on the nitrosamines and quality of traditional Chinese bacon
    Food Funct. (IF 3.289) Pub Date : 2019-01-02
    Jieyu Wu, Rongfa Guan, Haizhi Huang, Zhenfeng Liu, Haitao Shen, Qile Xia
    更新日期:2019-01-16
  • One-step assembly of zein/caseinate/alginate nanoparticles for encapsulation and improved bioaccessibility of propolis
    Food Funct. (IF 3.289) Pub Date : 2018-12-26
    Hao Zhang, Yuying Fu, Yujuan Xu, Fuge Niu, Zeya Li, Chujie Ba, Bing Jin, Guowen Chen, Xiaomeng Li
    更新日期:2019-01-16
  • Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio)
    Food Funct. (IF 3.289) Pub Date : 2019-01-15
    Mohamed A Gedi, Kieran Magee, Randa Darwish, Patchaniya Eakpetch, Iain Young, David Gray

    In recent years increasing aquaculture production combined with a high variance in availability and cost of fishmeal and fish oil commodities, has led to a need to identify alternative source materials for protein and the polyunsaturated fatty acids (PUFAs) which fish oil is prized for containing. Chloroplasts are the organelles in plants’ leaves where many of the valuable nutrients, fatty acids (FAs), amino acids, vitamins and pigments, are synthesised. Chloroplasts could be incorporated into fish diets either retained in, or liberated from, plant cells. In this study zebrafish were fed with seven different diets individually; fish was fed with reducing fishmeal levels (10, 20 or 50%) by either spinach leaf powder (SLP) or a chloroplast rich fraction (CRF) prepared by an established method to recover chloroplasts. Both SLP and CRF had a positive impact on growth, taste response, whole fishFA composition, and carotenoid profile. Fish fed with CRF diets showed significantly (P≤0.05) greater α-linolenic (C18:3 n-3) and hexadecatrienoic (C16:3) acids content than those of SLP and the control. Hexadecanoic acid (C16:3) is a unique FA in the galactolipids of the chloroplast; its presence in zebrafish tissues proves that zebrafish digest and absorb chloroplast galactolipids. Lutein profile of eggs produced by zebrafish fed with CRF diet was significantly (P≤0.05) higher than that of SLP and control. Alterations in egg colour were also noted, warranting further investigations of diet impacts on fish fecundity, embryo fertility, hatch rate and larval survival.

    更新日期:2019-01-16
  • Nanoencapsulation of curcuminoids extracted from Curcuma longa L. and evaluation of cytotoxic, enzymatic, antioxidant and anti-inflammatory activities
    Food Funct. (IF 3.289) Pub Date : 2019-01-15
    Priscila Dayane de Freitas Santos, Cristhian Rafael Lopes Francisco, Aline Coqueiro, Fernanda V Leimann, José Pinela, Ricardo C. Calhelha, Rafael Porto Ineu, I. C. F. R. Ferreira, Evandro Bona, Odinei Hess Gonçalves

    Curcumin, bisdemethoxycurcumin and demethoxycurcumin are the main curcuminoids present in Curcuma longa L. and are known for their bioactivity. However, their low water solubility results in poor bioavailability and therapeutic efficacy. This work aimed to investigate the in vitro modulation capacity on the enzymes acetylcholinesterase (AChE) and glutathione S-transferase (GST), as well as the in vitro antioxidant (OxHLIA and TBARS) and anti-inflammatory activities (RAW 264.7 test) of nanoencapsulated curcuminoids. Cytotoxicity on tumor and non-tumor cell lines was also investigated. Curcuminoids nanoparticles significantly inhibited the in vitro activity of AChE (12% inhibition at 50 μM) and GST (30% inhibition at 5 μM). They presented antioxidant activity and toxic effects against breast adenocarcinoma, lung, cervical and hepatocellular carcinoma cells when dispersed in water. Encapsulated curcuminoids exhibited bioactive properties in aqueous medium (no hydrophobic solvent added), exerting antioxidant and cytotoxic effects and acting on the cholinergic and endogenous antioxidant systems.

    更新日期:2019-01-16
  • Agaricus blazei Murrill from Brazil: an ingredient for nutraceutical and cosmeceutical applications
    Food Funct. (IF 3.289) Pub Date : 2019-01-15
    Taofiq Oludemi, Francisca Rodrigues, Lillian Barros, Rosane Peralta, Filomena Barreiro, I. C. F. R. Ferreira, Beatriz Oliveira

    Operations for mushrooms’ sorting at industrial level usually generate large amounts of bio-residues not conforming strict morphological criteria for commercial purposes, even though their biological content is not compromised. In this context, the present work aimed at evaluating the potential for reutilizing industrially discarded Agaricus blazei Murill (ABM). Thus, content in essential nutrients and chemical composition was determined, and MTT and LDH assays used to evaluate the viability and cell death in Caco-2 and HT29 cells lines of an ethanolic extract prepared from ABM (preliminary safety tests for nutraceutical applications). The extract was incorporated into a semi-solid base cosmetic cream and cell viability effects of the extract, and of the final cream formulation, studied in a keratinocytes cell line (HaCaT) (preliminary safety tests for cosmeceutical applications). Essential nutrients, such as proteins, carbohydrates and a low-fat content were determined for ABM. Twenty-two fatty acids were detected with polyunsaturated fatty acids (PUFA) (~53 %) as the most abundant fraction. The cell viability of Caco-2 and HT29 cells were maintained up to 100 µg/mL. After incorporation in the base cream, a formulation with a pale-yellow colour and favourable pH was obtained. The cell viability of HaCaT cells in the presence of the extract and final cream formulation was maintained according to a concentration dependent manner, which indicates the safety of this extract for cosmeceutical uses. The results suggest that ABM residues can be used as an inexpensive and sustainable source of nutraceutical and cosmeceutical ingredients.

    更新日期:2019-01-16
  • Comparative studies of DHA-enriched Phosphatidylcholine and recombination of DHA-ethyl ester with egg Phosphatidylcholine on ameliorating memory and cognitive deficiency in SAMP8 mice
    Food Funct. (IF 3.289) Pub Date : 2019-01-15
    Chengcheng Wang, Ying Guo, Miaomiao Zhou, Changhu Xue, Yaoguang Chang, Tiantian Zhang, Yuming Wang

    Docosahexaenoic acid-enriched phospholipids (DHA-PLs) are important bioactive components from marine foods, which are barely obtained by the inland people due to limited sources in comparison with commercial fish oil. Therefore, it is of significance to develop substitutions of DHA-PLs. In the present study, we comparatively investigated the effects of DHA-phosphatidylcholine (PC) and recombination of DHA-ethyl ester (EE) with egg PC on improving dysfunction of memory and cognition in the senescence-accelerated prone 8 (SAMP8) mice and illustrated the possible underlying mechanisms. Results showed that DHA-PC exhibited stronger effects than recombination of DHA-EE with egg PC on improving dysfunction of memory and cognition via suppressing Aβ generation, neuro-inflammation and apoptosis, and improving neurotrophins. These findings suggested that DHA-PLs (DHA-PC) could not be substituted by recombination of commercial fish oil with DHA-free PC in alleviating age-related memory loss and cognitive deficiency in SAMP8 mice, which provided a reference for the prevention and treatment of neurodegenerative diseases.

    更新日期:2019-01-16
  • An anticoagulant peptide from beta-casein: identification, structure and molecular mechanism
    Food Funct. (IF 3.289) Pub Date : 2019-01-15
    Hanxiong Liu, Maolin Tu, Shuzhen Cheng, Hui Chen, Zhenyu Wang, Ming Du

    Various bioactive peptides are identified from casein hydrolysates. YQEPVLGPVR (PICA), a novel antithrombotic activity peptide, derived from the fragment residues 193-202 of Beta-casein was identified by HPLC-LC-MS/MS. The anticoagulation activity assay showed that this peptide has strong antithrombotic activity. It was proved that this peptide indirectly inhibits the thrombin by interacting the active site of thrombin, it inhibited thrombin activity by binding the exosite-1 of thrombin, which was also confirmed by the fibrinogen clotting time assay. Result showed that PICA had antithrombotic activity and prolonged fibrinogen clotting time in a dose-dependent manner. Secondary structures of thrombin and thrombin-PICA complex were also been measured by circular dichroism (CD) to prove the fact that PICA can combine with thrombin. Moreover, Discovery Studio 2017 R2 software (DS 2017 R2) was used for molecular docking to provide the potential mechanism for antithrombotic activity of the peptide. These results suggested that PICA probably can be used as an antithrombotic ingredient in food industry.

    更新日期:2019-01-16
  • Uncovering the anti-proliferation mechanism and bioactive compounds in Red kidney bean coat against B16-F10 Melanoma Cells by metabonomic and network pharmacology analysis.
    Food Funct. (IF 3.289) Pub Date : 2019-01-15
    Jiahui Nie, Jianxiang Huang, Qingrong Wu, Xue Mei Qin, Zhenyu Li

    In this study, the coat (RKBC) and kernel (RKBK) of red kidney bean were prepared, and their chemical compositions and anti-proliferation activity against B16-F10 mice melanoma cells were compared. Then the anti-proliferation mechanisms of the active RKBC extract were investigated by cellular metabonomics and network pharmacology. The results showed that RKBC extract could inhibit the B16-F10 cells proliferation and migration in a dose dependent manner, and inhibit B16-F10 cell cycle arrest at the S phase and promoted cell apoptosis. Metabolomics and network pharmacology revealed that RKBC probably increased apoptosis rate by elevating cGMP and effected on B16-F10 cells cycle by inhibiting the expression of CDK2 and CDK4. Cyanidin-3-O-glucoside, kaempferol, and quercetin, were probably served as the major active components in the RKBC extract. The present study showed the potential of the RKBC extract for the prevention or treatment of melanoma. However, the mechanism and the corresponding active components of RKBC are required to validate in the future study.

    更新日期:2019-01-16
  • Nutritional and physical characterization of sugar-snap cookies: effect of banana starch in native and molten states
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Laura Roman, Marta Sahagun, Manuel Gomez, Mario M. Martinez
    更新日期:2019-01-16
  • 更新日期:2019-01-16
  • Emulsifying Properties of Water-Soluble Proteins Extracted from the Microalgae Chlorella sorokiniana and Phaeodactylum tricornutum
    Food Funct. (IF 3.289) Pub Date : 2019-01-14
    Sandra Ebert, Lutz Grossmann, Jörg Hinrichs, Jochen Weiss

    This study describes the composition and the impact of lyophilized water-soluble protein extracts from two different microalgae species on emulsion formation and stability. Lyophilized soluble proteins from Chlorella sorokiniana and Phaeodactylum tricornutum with a protein content of 39.2 and 37.2 wt%, respectively, were used. Drop-shape analysis showed them to have considerable interfacial activity at the oil water interface. The application in emulsions, prepared by high pressure homogenization (1,000 bar, 3 passes, 5.0 wt% oil) further revealed that a concentration of 1.0 wt% soluble protein from Chlorella sorokiniana was sufficient to manufacture an emulsion with a monomodal droplet size distribution and a small volume based mean particle diameter (d43 = 232±22 nm). Emulsions remained stable throughout 7 days of storage (d43,7d = 265±4 nm). In contrast, 3.7 wt% of the respective proteins from Phaeodactylum tricornutum were needed to obtain a stable emulsion (d43 = 334±12 nm and d43,7d = 325±8 nm). Emulsions prepared with both algae fractions showed unusually high salt stabilities up to 500 mM of sodium chloride, with no appreciable changes in volume based mean particle diameter, appearance, or microstructure. Furthermore, model emulsions with soluble lyophilized proteins from Chlorella sorokiniana had a very high stability toward changes in pH (pH ≥ 5), whereas soluble proteins of Phaeodactylum tricornutum showed only a moderate pH stability with the smallest volume based particle size at pH 7.

    更新日期:2019-01-14
  • Whey protein isolate-low methoxyl pectin nanocomplexes improve physicochemical and stability properties of quercetin in a model fat-free beverage
    Food Funct. (IF 3.289) Pub Date : 2019-01-14
    Wahyu Wijaya, Rachel Catherina Harfieyanto, Koen Dewettinck, Ashok R. Patel, Paul Van der Meeren

    In this study, whey protein isolate (WPI)-low methoxyl pectin (LMP) electrostatic complexes were used to encapsulate quercetin (Q) in a model fat-free beverage system. The effect of pH and WPI:LMP ratio was first studied to form soluble complexes with optimal physical properties, in terms of hydrodynamic diameter, surface charge, and yield. Based on the results, pH 5.0 and a 2:1 (w/w) ratio of WPI:LMP were selected for encapsulation of Q. The stoichiometry of the binding (n) and the binding constant (Kb) of WPI:Q were evaluated at pH values of 5.0 and 7.0 at room temperature. The Q-loaded WPI:LMP nanocomplexes were produced by mixing WPI with Q at two loading concentrations corresponding to 5:1 and 1:1 WPI:Q molar mixing ratios, followed by addition of LMP and pH adjustment to 5.0. The microstructure of Q-loaded WPI:LMP complexes was investigated by cryo-SEM imaging. Q was efficiently entrapped at two loading concentrations with an efficiency of about 97%. Q-loaded WPI:LMP complexes showed a physical stability during storage, and high temperature processing, as well as in the presence of challenging formulation conditions such as a high sugar concentration or salt addition (at a limited concentration). The stability of encapsulated Q against UV irradiation was approximately 4 times better than that of free Q. Moreover, Q-loaded WPI:LMP complexes were also lyophilized into dry powder, which they can be useful for practical application in food products.

    更新日期:2019-01-14
  • Anti-diabetic activity of canophyllol from Cratoxylum cochinchinense (Lour.) Blume in type 2 diabetic mice by activation of AMP-activated kinase and regulation of PPARγ
    Food Funct. (IF 3.289) Pub Date : 2019-01-14
    Yibing Lv, Qian Ming, Ji Hao, Yun Huang, Hao Chen, Qiang Wang, Xinzhou Yang, Ping Zhao

    In the present study, the potential anti-diabetic activity of canophyllol (CNPL) and its mechanisms were investigated in vitro and in vivo. CNPL is a predominant friedelane-type triterpene extracted from young leaves of Cratoxylum cochinchinense (“Huang Niu Tea”). In vitro, CNPL increased GLUT4 translation in a L6-myotube model to 2.60-fold, and glucose uptake 1.71-fold levels relative to untreated L6 myotubes. Moreover, Compound C, an inhibitor of AMP-activated protein kinase (AMPK), completely suppressed the CNPL-induced GLUT4 translocation. In vivo, serum insulin levels, hyperglycemia, as well as other blood indexes of the KK-Ay diabetic mice were improved by a four-week oral administration of CNPL. Examination of its possible anti-diabetic mechanism showed that CNPL promoted the expression of p-AMPKα and GLUT4 in L6 myotubes. In KK-Ay mice treated with CNPL, GLUT4 expression was increased in skeletal muscle and white adipose tissue, and levels of p-AMPKα was increased in liver, skeletal muscle and white adipose tissue. Moreover, CNPL stimulated the expression of PPARγ to decrease and inhibited the activity of ACC by stimulating its phosphorylation in both liver and white adipose tissue, thereby indicating that CNPL also improved lipid metabolism disorders. Combined, these results show that CNPL may improve glucose tolerance, reduce hyperglycemia, normalize insulin secretion, and ameliorate lipid metabolism disorders. Diverse anti-diabetic effects of CNPL may be attributed to regulation of GLUT4, PPARγ, AMPK phosphorylation and ACC phosphorylation.

    更新日期:2019-01-14
  • Supplementation with Lactobacillus kefiranofaciens ZW3 from Tibetan Kefir improves depression-like behavior in stressed mice by modulating the gut microbiota
    Food Funct. (IF 3.289) Pub Date : 2019-01-14
    ye sun, Weitao Geng, Yajing Pan, JinJu Wang, Ping Xiao, Yanping Wang

    Increasing evidence indicates that probiotics can effectively improve depression-like behavior. However, the underlying mechanism is still unclear. In this study, the antidepressant effect of Lactobacillus kefiranofaciens CGMCC2809 (ZW3) isolated from Tibetan Kefir grains was investigated using a mouse model of chronic unpredictable mild stress (CUMS). ZW3 improved depression-like behavior and independent exploration ability in the CUMS group. Moreover, ZW3 regulated biochemical disorders in the hypothalamic–pituitary–adrenal axis, immune system and tryptophan metabolism caused by stress. Furthermore, ZW3 could modulate the composition of the gut microbiota, and alleviate constipation by improving the fecal water content in stressed mice. We found that the probiotic strain was present in the whole intestine, even 7 days after administration was stopped. These results suggest that L. kefiranofaciens ZW3 might improve depression by regulating gut microbiota as a probiotic food.

    更新日期:2019-01-14
  • Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide
    Food Funct. (IF 3.289) Pub Date : 2019-01-10
    Xiuying Wang, Wenjun Wang, Longmei Wang, Cheng Yu, Guolong Zhang, Huiling Zhu, Chunwei Wang, Shengjun Zhao, Chien-An Andy Hu, Yulan Liu
    更新日期:2019-01-14
  • D- and L-Aspartate Regulates Growth Performance, Inflammation and Intestinal Microbial Community in Young Pigs
    Food Funct. (IF 3.289) Pub Date : 2019-01-11
    Yuying Li, Hui Han, Jie Yin, Xingguo He, Zhiyi Tang, Tiejun Li, Kang Yao, Yulong Yin

    D-aspartate (D-Asp), an endogenous amino acid, is widely occurred in the animal and human with D-enantiomers and plays an important role in the endocrine and nervous system. However, little is known about growth performance, microbial community, and intestinal immune and inflammatory status in response to D- and L-aspartate (L-Asp). Thus, this study mainly investigated the effects of dietary 1% D- and L-Asp on growth performance, inflammation, and microbial community in young pigs. Twenty-eight young pigs were randomly divided into four groups (n=7): a control group in which piglets were fed a basal diet and other three groups in which piglets received 1% D-Asp, 1% L-Asp, and 1% DL-aspartate (DL-Asp) for 35 days, respectively. The results showed that dietary 1% D-Asp significantly inhibited average daily feed intake and average daily gain. Gut microbes were tested and the results showed that L-Asp enhanced bacterial diversity (Shannon and Simpson). At the phylum level, L-Asp enhanced intestinal Actinobacteria and Bacteroidetes abundances but decreased Firmicutes abundance. In contrast, DL-Asp decreased intestinal Actinobacteria and Bacteroidetes abundances and increased Firmicutes abundance. At the genus level, D-Asp enhanced Clostridium sensu stricto 1 and Intestinibacter abundances. Metagenomic predictions by PICRUSt suggested that the altered microbiota mainly involved in membrane transport, carbohydrate metabolism, amino acid metabolism, replication and repair, translation, and nucleotide metabolism. In addition, DL-Asp markedly increased serum alanine aminotransferase, aspartate transaminase and alkaline phosphatase activity. Also, dietary D -and DL-Asp were down-regulated TLR 4, NOD1, and MyD88 in the jejunum to mediate inflammatory response. Collectively, these results indicated that dietary D-Asp and L-Asp affected growth performance and inflammation in piglets, which might be associated with gut microbiota.

    更新日期:2019-01-13
  • Lactulose synergizes with CpG-ODN to modulate epithelial and immune cells cross talk
    Food Funct. (IF 3.289) Pub Date : 2019-01-04
    R. Mukherjee, M. van de. Kaa, J. Garssen, R. J. Pieters, A. D. Kraneveld, L. E. M. Willemsen
    更新日期:2019-01-13
  • Identification of a new natural gastric lipase inhibitor from star anise
    Food Funct. (IF 3.289) Pub Date : 2018-11-24
    Jannet Kamoun, Renaud Rahier, Mohamed Sellami, Imed Koubaa, Pascal Mansuelle, Régine Lebrun, Alexandra Berlioz-Barbier, Michele Fiore, Karine Alvarez, Abdelkarim Abousalham, Frédéric Carrière, Ahmed Aloulou
    更新日期:2019-01-13
  • Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats
    Food Funct. (IF 3.289) Pub Date : 2019-01-10
    Fengzheng Gao, Wei Guo, Mingyong Zeng, Yinong Feng, Guangxin Feng

    Microalgae are potential iron supplements for iron deficiency improvement with unknown mechanism. To analyze the enhancement of non-heme iron absorption by microalgae, in current paper, six different microalgal feeds were prepared as main source of dietary iron with 25 mg/kg iron from Spirulina, Chlorella and Synechococcus sp. PCC 7002 (named as H-Sp, H-Ch and H-Sy, respectively) or portion source of dietary iron with 5 mg/kg iron from these three microalgae (named as L-Sp, L-Ch and L-Sy, respectively) to subdue iron deficiency anemia in rats. The hemoglobin regeneration efficiencies in anemic rats followed the order: ferric citrate (34.7 ± 1.8%) < H-Ch (49.9 ± 4.1%) ≈ H-Sy (50.6 ± 5.3%) ≈ L-Sp (46.9 ± 6.2%) ≈ L-Ch (43.1 ± 6.9%) ≈ L-Sy (43.5 ± 2.4%) ≈ FeSO4 (47.2 ± 4.9%) < H-Sp (54.8 ± 5.5%). The percentage content of intestinal nanosized iron in the groups of H-Sp, H-Ch and H-Sy was significantly higher than that in L-Sp, L-Ch and L-Sy groups; moreover, it was significantly higher in groups of microalgal diets than in the ferric citrate group, providing strong evidence for nanosized iron supplementation from microalgae. Overall, microalgae, especially Spirulina, are functional iron nutritive fortifiers which could supply more intestinal nanosized iron.

    更新日期:2019-01-11
  • Polysaccharides from fermented Momordica charantia ameliorate obesity in high-fat induced obese rats
    Food Funct. (IF 3.289) Pub Date : 2019-01-10
    Jia-Jia Wen, He Gao, Jie-Lun Hu, Qi-Xing Nie, Hai-Hong Chen, Tao Xiong, Shao-Ping Nie, Ming-Yong Xie
    更新日期:2019-01-11
  • Germinated soy germ extract ameliorates obesity through beige fat activation
    Food Funct. (IF 3.289) Pub Date : 2019-01-10
    Han-Jun Kim, Eun-Ji Choi, Hyo Sung Kim, Chanwoong Choi, Sik-Won Choi, Sun-Lim Kim, Woo Duck Seo, Sun Hee Do

    Obesity is a worldwide public health concern requiring safe and effective strategies. Recent studies suggest that bioactive compounds from soybeans have beneficial effects on weight loss and reducing fat accumulation. However, despite the biochemical and nutritional changes during germination, the biological effects of germinated soy germ were not fully investigated. In this article, germinated soy germ extract (GSGE) was evaluated as a potential treatment options for obesity using 3T3-L1 pre-adipocyte and high-fat diet (HFD)-induced obese mice. In vitro studies demonstrated that GSGE suppressed differentiation of 3T3-L1 cells into mature adipocytes, along with reductions in lipid accumulation and lipid droplet formation. In vivo studies also showed that a daily dose of 1 mg/kg of GSGE reduced weight gain, adipocyte area, serum triglyceride, and LDL-cholesterol in HFD-fed mice. The GSGE treatment promoted browning, which was associated with an increased UCP1 expression in vitro and in vivo. In addition, GSGE treatment induced beige fat activation by upregulation of lipolysis and beta-oxidation. Furthermore, gene and protein expression levels of endocannabinoid system-related factors such as NAPE-PLD, FAAH, DAGL-α, and CB2 were altered along with browning and beige fat activation by GSGE. The present study indicates that GSGE effectively inhibiting lipid accumulation and promoting beige fat transition and activation. Our results demonstrated that GSGE treatment represents a promising strategy for the prevention of obesity by promoting weight loss, reducing fat accumulation, and improving obesity-related metabolic disorders.

    更新日期:2019-01-11
  • Effects of high pressure homogenization and addition of oil on the carotenoid bioaccessibility of carrot juice
    Food Funct. (IF 3.289) Pub Date : 2018-12-20
    Jianing Liu, Jinfeng Bi, Xuan Liu, Baiqing Zhang, Xinye Wu, Chandi Kanchana Deepali Wellala, Biao Zhang
    更新日期:2019-01-11
  • Antiplatelet aggregation and antithrombotic benefits of terpenes and flavones from hawthorn leaf extract isolated using activity-guided method
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Pinyi Gao, shuangshuang Li, Ke-Chun Liu, Chen Sun, Shaojiang Song, Lingzhi Li

    Hawthorn is a well-known functional food, at present, increasing attention has been given to hawthorn leaf due to its numerous functional and nutritional properties. In this study, the antithrombotic properties of hawthorn leaves were evaluated using the activity-guided isolation process and high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). A crude extract prepared in 75% ethanol was fractionated using macroporous resin D101 and polyamide chromatography to obtain three active fractions (Fr.C, Fr.C-1 and Fr.C-2). Thereafter, the inhibitory activities of these fractions were examined by platelet aggregation and antithrombus assays using a zebrafish model. Using the HPLC-QTOF-MS technique, we identified 25 compounds in the active fraction (Fr.C). The structures of these compounds were identified by comparing the retention time (tR) and mass spectral data from the previous reports. Based on the analysis, 21 peaks were detected in the mass spectrum of Fr.C-1 and 8 peaks were detected in Fr.C-2, We found that 11 compounds in Fr.C-1 exhibited potent inhibitory effects on platelet aggregation, including nine monoterpenoids, one diterpenoid and one flavanone, Accordingly, monoterpenoids are suggested as the main anti-platelet aggregation constituents from hawthorn leaves. Particularly, compounds 10 and 24 inhibited the ADP-induced platelet aggregation and delayed the FeCl3-induced thrombus in zebrafish. Further, interactions between compounds 10 and 24 with two ADP receptors P2Y1 and P2Y12, serving as the target for key regulators of antiplatelet aggregative activity, were investigated via molecular modeling. In addition, five flavones were obtained from the active fraction (Fr.C-2). These results indicated that monoterpenoids glycosides and some flavones were responsible for the antithrombotic activity of hawthorn leaves. Moreover, this study shows that the activity-guided isolation, is a fast, efficient and systematic separation method, for identification of active compounds in natural products.

    更新日期:2019-01-11
  • Luteolin inhibits proliferation and induces apoptosis of human melanoma cell in vivo and in vitro by suppressing MMP-2 and MMP-9 through PI3K/AKT pathway
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Xin Yao, Wei Jiang, Danhong Yu, Zhaowei Yan

    The incidence rate of malignant melanoma is increasing annually, drug development against melanoma cell metastasis becomes more urgent. Luteolin, a naturally occurring flavonoid, is abundant in our daily dietary intake and exhibits a wide spectrum of pharmacological properties. However, the potential anti-cancer role of luteolin in melanoma cell has not been fully investigated. The study is to explore whether luteolin inhibits migration and invasion of A375 human melanoma cell, and further elucidate underlying anti-cancer molecular mechanism of luteolin in melanoma cell. Proliferation assay, flow cytometry and apoptosis assay were applied to detect the effect of luteolin on the growth and apoptosis of A375 cell. Wound healing assay and transwell invasion assay were used to explore the impact of luteolin on the migration and invasion of A375 cell. Real-time quantitative PCR, western blot and immunofluorescence analysis were used to investigate the effects of luteolin on the expressions of MMP-2, MMP-9 and PI3K/AKT1 in A375 cell. Xenograft tumor animal model was conducted to investigate the anti-cancer effect of luteolin on the growth of A375 cell in vivo. Our data indicated that luteolin significantly inhibited proliferation, migration and invasion of A375 cell, and induced apoptosis of A375 cell in a concentration-dependent manner. Also, luteolin reduced the expressions of MMP-2, MMP-9 and increased the expressions of TIMP-1 and TIMP-2. Furthermore, luteolin significantly inhibited tumor growth of A375 cell in xenograft mice model. The immunofluorescence and immunoblotting assays indicated that luteolin inhibited the phosphorylation of AKT1 and PI3K. In conclusion, both in vivo and in vitro studies showed that luteolin inhibited proliferation and induced apoptosis of A375 human melanoma cell by reducing the expressions of MMP-2 and MMP-9 through the PI3K/AKT pathway. Taken together, luteolin can be considered as a promising anti-cancer agent for the treatment of human melanoma.

    更新日期:2019-01-11
  • The protective role of hawthorn fruit extract against high salt-induced hypertension in Dahl salt-sensitive rats: impact on oxidative stress and metabolic patterns
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Xuewei Zheng, Xiaoxue Li, Meng Chen, Pengfei Yang, Xinrui Zhao, Li Zeng, Yanan Ouyang, Zhe Yang, zhongmin Tian

    In the present study, the renal-protective effect of hawthorn fruit extract (HW) in high-salt hypertension and its effect on metabolic patterns are determined. High salt causes hypertension in Dahl salt sensitive (SS) rats, while HW can effectively attenuate high-salt induced hypertension. And, various antihypertensive ingredients of HW are also successfully identified using GC/MS. Of note, biochemical assay indicates HW significantly increases the concentration of nitric oxide (NO), decreases concentration of H2O2 and malonaldehyde. Especially HW increases the activities of NO synthase and catalase in renal medulla. Simultaneously, the renal cortex and medulla, harvested from SS rats, are used to perform the metabolomics analysis, and then, 11 and 8 differential metabolites are identified in the renal medulla and cortex with the HW gavage, respectively. All differential metabolites are then performed pathway enrichment analysis. The results show that many metabolic pathways enriched in both renal medulla and cortex, especially these in medulla including 23 enriched pathways. Therefore, it provides evidence that HW confers antioxidant effect on high-salt induced hypertension and dramatically alters the metabolic patterns of SS rats. And antihypertensive ingredients of HW also further indicate it may be used as a nutritional supplemental therapeutic drug to protect against high-salt induced hypertension in renal medulla.

    更新日期:2019-01-09
  • Cyanidin Inhibits EMT Induced by Oxaliplatin via Targeting the PDK1-PI3K/Akt Signaling Pathway
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Hao Wang, Xiang Li, Zesheng Zhang, xiaohan Zhang, shengnan Yang, Dong Liu, Cuiru Diao

    Anthocyanins have been shown to exhibit antitumor activity on several cancers in vitro and in vivo. Oxaliplatin is wildly used as an anti-cancer drug. However, a large proportion of patients receiving platinum-based anti-cancer drug treatments will relapse because of metastasis and drug resistance. The aim of the study is to discover an effective anthocyanin that possesses the combinational anti-metastatic effects of oxaliplatin. Our results showed that cyanidin, one of the main constituent of anthocyanins, widely found in black rice, black bean, Hawthorn and other foods, could reverse drug resistance and enhance the effects of oxaliplatin in hepatic cellular cancer (HCC). Cyanidin inhibited migration and reversed EMT biomarker changes induced by low dose OXA. Moreover, 3-phosphoinositide-dependent protein kinase 1 (PDK1) can be considered a potential target and cyanidin significantly increased OXA sensitivity and inhibited EMT induced by OXA via PI3K/Akt signaling in HCC.

    更新日期:2019-01-09
  • Lysine-induced swine satellite cell migration is mediated by the FAK pathway
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Chenglong Jin, Zongming Zhang, Jinling Ye, Chunqi Gao, Huichao Yan, Haichang Li, Jin-zeng Yang, Xiu-Qi Wang

    Lysine (Lys) is an essential amino acid for mammals in promoting protein synthesis and skeletal muscle growth. However, the underlying mechanism by which Lys governs muscle growth remains unknown. Lys is not only a material for protein synthesis but also a signaling molecule. Cell migration is a fundamental process for satellite cell (SC) to promote muscle fiber hypertrophy and thus increase muscle mass. Nevertheless, the communication between Lys and SC has not yet attracted sufficient attention. In this study, we investigated whether Lys directly stimulates SC migration and whether this effect is mediated via the focal adhesion kinase (FAK) pathway. The results of a cell wound-healing assay and transwell assays indicated a significant inhibition of migration ability by Lys deficiency. In addition, the phosphorylation of FAK, paxillin and protein kinase B (Akt) was significantly suppressed, as were the level of integrin β3. Fortunately, we found that increasing Lys levels from deficiency to sufficiency rescued the migration ability to the control level. Moreover, compared with those in the Lys-deficiency group, the proteins in the FAK pathways were reactivated in the Lys-resupplementation group. In conclusion, these findings indicate that the FAK pathway mediates Lys-induced SC migration.

    更新日期:2019-01-09
  • Dietary Wood Pulp-Derived Sterols Modulates Cholesterol Metabolism and Gut Microbiota in High-Fat Diet Fed Hamsters
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    xiang li, Huali Wang, Tianxin Wang, Fuping Zheng, Hao Wang, Chengtao Wang

    Evidence indicates that Wood Pulp-Derived Sterols (WS) has beneficial effects on cardiovascular diseases. The present study was to (i) investigate the serum cholesterol-lowering activity of dietary WS; (ii) investigate the effect of dietary WS on the balance of gut microbiota and its regulation mechanism in hamsters fed on a high-fat diet. Thirty-six hamsters were divided into four groups fed on a normal chow diet (NCD), a high-fat diet (HFD), HFD plus 0.1 % or 0.5 % wood pulp-derived sterols (WSL, WSH), respectively, for 6 weeks. Levels of serum total cholesterol, triglyceride, HDL-C, non-HDL-C and non-HDL-C / HDL-C ratio in hamsters for the NCD originally were 120.4, 235.8, 71.7, 48.7mg / dL and 0.68, which were elevated by HFD to 187.7, 389.5, 92.3, 95.3 mg / dL and 1.03, and alleviated completely by WSH. The excretion of total fecal neutral sterols was dose-dependently increased with the amounts of dietary WS. Furthermore, Dietary supplementation with WS modulated the relative abundance of gut microbiota compared with the HFD group. The Spearman’s correlation analysis revealed that Bacteroides, Allobaculum, Coprobacillus, Lactobacillus, Akkermansia, Coprococcus and Oscillospira were correlated negatively with most of the serum metabolic parameters and cholesterol metabolic parameters, whereas Desulfovibrio was positively correlated with most of the lipid metabolism-associated parameters. Taken together, dietary supplementation with WS was found to have cholesterol-lowering activity, in part mediated by modulating gut microbiota in a positive way and regulating the cholesterol absorption and metabolism-related genes.

    更新日期:2019-01-09
  • High Linoleic Acid Diet Exacerbates Metabolic Responses and Gut Microbiota Dysbiosis in Obese Rats with Diabetes Mellitus
    Food Funct. (IF 3.289) Pub Date : 2019-01-09
    Hsiu-Chuan Lee, Shao-Chuan Yu, Yun-Chun Lo, I-Hsuan Lin, Te-Hsuan Tung, Shih-Yi Huang

    Dietary polyunsaturated fatty acids (PUFA) levels may affect inflammatory responses and lipid metabolism. Gut microbiota divsrsity are strongly associated with chronic inflammatory disease, diabetes mellitus (DM), and obesity through abnormal energy homeostasis. In this study, the association between metabolic responses and gut microbiota diversity at different dietary n-6/n-3 PUFA ratios was evaluated in DM rats. Obesity and DM were induced in the rats by using a high-fat diet and streptozotocin (STZ), respectively. The obese DM rats were assigned to three groups and administered to regular (R), high (H), and low (L) n-6/n-3 ratio diets (n-6/n-3 = 6.39, 3.02, and 9.29, respectively) for 6 weeks. Some metabolic parameters and gut microbiota of the rats were analysed. The results revealed that high n-6/n-3 ratio diet increased the plasma and kidney interleukin 6 levels, whereas low n-6/n-3 ratio diet ameliorated blood glucose homeostasis, reduced plasma tumour necrosis factor α levels, and inhibited systematic inflammation. DM rats exhibited low gut microbiota diversity; however, compared with the R group, the L and H groups did not exhibit alterations in the α-diversity (Observed, Chao 1, Shannon and Simpson). The percentage of Firmicutes was lower in the DM groups than in the non-DM group; however, the L group showed a nonsignificantly higher Firmicutes/Bacteroidetes ratio than did other groups. Thus, low n-6/n-3 ratio diet can improve blood glucose homeostasis, reduces systematic inflammation, ameliorate glomerular basal membrane thickening, reduce the expression of receptors of advanced glycation end products in the renal vessel walls, and prevent diabetic nephropathies.

    更新日期:2019-01-09
  • Pharmacodynamic and urinary metabolomics studies on the mechanism of Schisandra polysaccharide in the treatment of Alzheimer's disease
    Food Funct. (IF 3.289) Pub Date : 2018-12-24
    Yuanyuan Liu, Zhongying Liu, Mengying Wei, Mingxin Hu, Kexin Yue, Rongbing Bi, Shan Zhai, Zifeng Pi, Fengrui Song, Zhiqiang Liu
    更新日期:2019-01-09
  • Impact of emulsifier nature and concentration on the stability of β-carotene enriched nanoemulsions during in vitro digestion
    Food Funct. (IF 3.289) Pub Date : 2019-01-08
    Ariadna Gasa-Falcon, Isabel Odriozola-Serrano, Gemma Oms-Oliu, O. Martín-Belloso

    The presence of emulsifiers facilitates the formation of nanoemulsions and helps on their stabilization. At the same time, the behaviour of nanoemulsions along the gastrointestinal tract mainly depend on their composition, affecting the bioaccessibility of the encapsulated compound. The goal of this work was to study how β-carotene-enriched nanoemulsions prepared with different emulsifiers (Tween 20, lecithin, sodium caseinate, sucrose palmitate) and concentrations (2-8%) would affect their stability (particle size and zeta-potential) during an in vitro gastrointestinal tract (GIT). The lipid digestibility, as well as the β-carotene bioaccessibility of nanoemulsions, was also determined. Nanoemulsions stabilised with Tween 20, lecithin and sodium caseinate did not present any variation in particle size under stomach conditions. After intestinal GIT phase, all nanoemulsions experienced physical changes, either increasing or reducing their particle size depending on the nature and concentration of emulsifier used. The zeta potential of all nanoemulsions was maintained negative throughout the GIT, being less negative after the stomach GIT phase (between -24.2 and -1.4 mV). Lecithin-stabilised nanoemulsions presented the highest number of free fatty acids when emulsifier concentration increased from 2 to 8%. In this sense, nanoemulsions containing 8% of lecithin exhibited the highest β-carotene bioaccessibility (23.5%), suggesting that lecithin can enhance lipid digestion and bioaccessibility of β-carotene encapsulated within nanoemulsions. This work elucidates the importance of not only the emulsifier nature but also the concentration used when designing nanoemulsions as delivery systems of lipophilic compounds.

    更新日期:2019-01-09
  • Non-volatile pungent compounds isolated from Zingiber officinale and their action mechanisms
    Food Funct. (IF 3.289) Pub Date : 2019-01-08
    Dabo Pan, Zeng Chen, Wei-Yang Zhang, Ting Li, Zifei Qin, xiaojun yao, Yi Dai, Zhihong Yao, Yang Yu, Xin-Sheng Yao

    In this study, an efficient strategy developed by integrating UPLC-Q/TOF-MS, network pharmacology, and molecular simulation, was proposed and applied for rapid screening bioactive candidates from the ginger. A UPLC-Q/TOF MS-guided isolation targeting non-volatile pungent compounds resulted in the isolation and identification of 19 compounds in the rhizome of Zingiber officinale, including six new compounds (1-6). Based on target prediction and Gene Ontology (GO), the primary biological function of compounds was predicted to associate with cancer and the key target was focused on VEGFR2 (vascular endothelial growth factor receptor 2). Moreover, cytotoxic activity assays demonstrated the isolated compounds had potential anti-proliferative effects on MDA-MB-231, A549 and HCT116 cells. Especially, compounds 7 and 8 exhibited the highest cytotoxic against HCT116 compared with the other cell lines, with IC50 values ranging from 4.70 to 7.40 μM. In addition, inhibiting VEGFR2 of compounds 7 and 8 were validated based on enzyme activity assay and their interaction mechanisms were illuminated through molecular simulations. These experimental data are consistent with the calculated results, indicating the veracity of the proposed method. In conclusion, the integrated strategy is a quick and efficient way to explore bioactive compounds as well as research the possible targets, providing us with a good possibility of screening new lead compounds from natural sources.

    更新日期:2019-01-09
  • The in silico and in vivo evaluation of puerarin against Alzheimer’s disease
    Food Funct. (IF 3.289) Pub Date : 2019-01-08
    Song Liu, Xiaolu Cao, Guangqi Liu, Tong Zhou, Xiliang Yang, Bingxin Ma

    The root of Pueraria lobata has been utilized as a food source for thousands of years in China. Puerarin is the major bioactive and the most abundant secondary metabolite from the root of P. lobate. The potential therapeutic effect of puerarin against Alzheimers was screened by in silico method and confirmed by the amyloid β-peptide-induced Alzheimer’s disease (AD) rats’ model. The in silico study displayed that puerarin had the potential to penetrate across the blood-brain barrier and had high stability in the molecular docking and dynamics simulation with acetylcholinesterase (AChE), cyclooxygenase-2 (COX-2) and caspase-3 (C3), which play a central role in the development of AD. The in vivo results showed that puerarin could restrain the AChE activity, restore the activities of antioxidant defense substances toward normal levels, decrease the expression of inflammatory factors and apoptosis genes in the brain, especially down-regulated the expressions of COX-2 and C3. The histopathological examination of brain sections and behavioral testing also verified the biochemical observations, which further validates the in silico study. These results not only suggest that puerarin, as a potential compound, could relieve AD, but also broaden the applications of puerarin.

    更新日期:2019-01-09
  • Biofunctionalization of selenium nanoparticle with a polysaccharide from Rosa roxburghii fruit and its protective effect against H2O2-induced apoptosis in INS-1 cells
    Food Funct. (IF 3.289) Pub Date : 2019-01-07
    Lei Wang, Chao Li, Qiang Huang, Xiong Fu

    Defective glucose-stimulated insulin secretion (GSIS) induced by chronic exposure to reactive oxygen species (ROS) is a hallmark of type 2 diabetes mellitus (T2DM). Therefore, it is of great interest to search for bio-functional agents with antioxidant activity to protect pancreatic islet cells from oxidative damage. In the present study, selenium nanoparticles (SeNPs) functionalized with a novel polysaccharide (RTFP-3) extracted from Rosa roxburghii fruit were first prepared via a facile, single-step and green in-situ synthesis method. The in vitro protective effects of RP3-SeNPs on INS-1 cells against H2O2-induced cell apoptosis were investigated. Structural characterization indicated that RTFP-3 functionalized SeNPs (RP3-SeNPs) with an average diameter of 104.5 nm was of highly uniform and extremely stable compared to bare SeNPs. Bioassay results revealed that RP3-SeNPs possessed much better protective and suppressed activities against H2O2-induced INS-1 cell apoptosis as compared to its single components. Mechanistic studies exhibited that RP3-SeNPs effectively blocked the overproduction of intracellular ROS, mitochondria damage, activation of caspase-3, -8, and -9 in INS-1 cells, indicating that RP3-SeNPs functioned through attenuating oxidative stress and down-regulation expression of uncoupling protein-2 (UCP-2). Our findings suggest that RP3-SeNPs can function as a promising candidate to prevent or attenuate dysfunction of β-cells.

    更新日期:2019-01-07
  • Geographical specific composition of cultured microbiota and Lactobacillus occurrence in human breast milk in China
    Food Funct. (IF 3.289) Pub Date : 2019-01-07
    Mengfan Ding, Ce Qi, Zhenyu Yang, Shan Jiang, Ye Bi, Jianqiang Lai, Jin Sun

    Breast milk bacteria play an important role in the early development of the gut microbiota and immune system. Dominant living bacteria of 89 healthy Chinese women from 11 cities in five regions were analysed by broad-range yeast extract, casitone, and fatty acid and de Man, Rogosa, and Sharpe-based culturing coupled with 16S rRNA sequence and quantitative polymerase chain reaction. Principal coordinates analysis showed that human breast milk samples were classified into three groups, driven by Enterococcus (abundance in group 1, 63.13%), Streptococcus (abundance in group 2, 68.16%) and Staphylococcus (abundance in group 3, 55.17%). The microbiota profile was highly region-specific. Samples from northwest and north of China showed higher alpha diversity compared to other regions (p < 0.05). Staphylococcus, Streptococcus, and Enterococcus were the dominant genera in all samples. Lactobacillus had high occurrence in samples from northwest and north, dominated by Lactobacillus reuteri and Lactobacillus gasseri. Samples of mothers with high postpartum body mass index showed more Staphylococcus and less Lactobacillus and Streptococcus. Staphylococcus was negatively correlated with Lactobacillus and Streptococcus. The mode of delivery also affected composition of microbiota, even after culture. These findings indicate differences between north and south, provide effective information for sample collection when Lactobacillus is the predominant genus, and lower the detection limit for small amounts of bacteria.

    更新日期:2019-01-07
  • Impact of different level of Cysteine on the plasma metabolomics and intestinal microflora of the sow during late pregnancy
    Food Funct. (IF 3.289) Pub Date : 2019-01-05
    Sujuan Ding, Jun Fang, Gang Liu, Duraipandiyan Veeramuthu, Al-Dhabi N.A., Yulong Yin

    Cysteine (Cys) is a semi-essential amino acid that can be synthesized from human and animal methionine and affects the specific physiological state of mammals. This study aims to investigate the effects of different level of Cys on the birth weight of piglet, biochemical parameters in plasma, intestinal microbial and plasma metabolism of sows during late pregnancy. The results showed that 0.4% Cys group improved the birth weight of piglets and decreased the level of calcium, triglyceride and bilirubin, but 0.5% Cys group reduced the activity of gamma-glutamyl transpeptidase and increased the concentration of glucose of sows at the point of farrowing. The result of intestinal microbial analysis showed that 0.4% Cys group increased the diversity of faecal intestinal microbes compared with 0.5% Cys group. In addition, plasma metabolomic results differentiated that the three group of sows and eleven discriminating metabolites were identified. 0.4% and 0.5% Cys group increased the hypotaurine, 0.5% Cys group increased the acetylcysteine, and there was no significant difference between 0.4% and 0.5% Cys group. Moreover, the Pearson analysis revealed a positive correlation between hypotanaurine and Lactobacillus or Pseudobutyrivibrio, respectively. However, there is a negative correlation between acetylcysteine and Ruminococcaceae_UCG-014. These results revealed that 0.4% Cys group increased the birth weight of piglets, increased the differential metabolites beneficial to the antioxidant stress of embryos and the enhancement of microbial abundance, and increased the diversity of fecal microbe, which demonstrated that 0.4% Cys is better for sow to maintain the health status during the late pregnancy.

    更新日期:2019-01-06
  • Dietary (−)-epicatechin affects NF-κB activation and NADPH oxidases in the kidney cortex of high-fructose-fed rats
    Food Funct. (IF 3.289) Pub Date : 2019-01-03
    Paula Denise Prince, Cecilia Rodríguez Lanzi, César G. Fraga, Mónica Galleano
    更新日期:2019-01-03
  • The protective role of phloretin against dextran sulfate sodium-induced ulcerative colitis in mice
    Food Funct. (IF 3.289) Pub Date : 2018-12-21
    Zecai Zhang, Shan Li, Hongyang Cao, Peng Shen, Jiuxi Liu, Yunhe Fu, Yongguo Cao, Naisheng Zhang
    更新日期:2019-01-03
  • Modification of wheat bran particle size and tissue composition affects colonisation and metabolism by human faecal microbiota
    Food Funct. (IF 3.289) Pub Date : 2018-12-20
    Kim De Paepe, Joran Verspreet, Mohammad Naser Rezaei, Silvia Hidalgo Martinez, Filip Meysman, Davy Van de Walle, Koen Dewettinck, Christophe M. Courtin, Tom Van de Wiele
    更新日期:2019-01-03
  • Comparative study on the physicochemical properties and bioactivities of polysaccharide fractions extracted from Fructus Mori at different temperatures
    Food Funct. (IF 3.289) Pub Date : 2018-12-20
    Jia-Qi Zhang, Chao Li, Qiang Huang, Li-Jun You, Chun Chen, Xiong Fu, Rui Hai Liu
    更新日期:2019-01-03
  • The ameliorative effect of a Lactobacillus strain with good adhesion ability against dextran sulfate sodium-induced murine colitis
    Food Funct. (IF 3.289) Pub Date : 2018-12-03
    Guangqiang Wang, Yingnan Liu, Zhi Lu, Yiting Yang, Yongjun Xia, Phoency F.-H. Lai, Lianzhong Ai
    更新日期:2019-01-03
  • Resveratrol improves intestinal barrier function, alleviates mitochondrial dysfunction and induces mitophagy in diquat challenged piglets1
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    Shuting Cao, Zhuojun Shen, Chunchun Wang, Qianhui Zhang, Qihua Hong, Yonghui He, Caihong Hu
    更新日期:2019-01-02
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  • Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets
    Food Funct. (IF 3.289) Pub Date : 2018-12-14
    Shuai Chen, Bie Tan, Yaoyao Xia, Simeng Liao, Meiwei Wang, Jie Yin, Jing Wang, Hao Xiao, Ming Qi, Peng Bin, Gang Liu, Wenkai Ren, Yulong Yin
    更新日期:2019-01-02
  • Theanine attenuates memory impairments induced by klotho gene depletion in mice
    Food Funct. (IF 3.289) Pub Date : 2018-12-14
    Bao Trong Nguyen, Naveen Sharma, Eun-Joo Shin, Ji Hoon Jeong, Sung Hoon Lee, Choon-Gon Jang, Seung-Yeol Nah, Toshitaka Nabeshima, Yukio Yoneda, Hyoung-Chun Kim
    更新日期:2018-12-21
  • Immunomodulatory activity of a water-soluble polysaccharide obtained from highland barley on immunosuppressive mice models
    Food Funct. (IF 3.289) Pub Date : 2018-12-12
    Lirong Han, Meng Meng, Mingzhu Guo, Dai Cheng, Lei Shi, Xu Wang, Chunling Wang
    更新日期:2018-12-21
  • 更新日期:2018-12-21
  • 更新日期:2018-12-21
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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