Various immune cells are present in the skin and modulate the cutaneous immune response. In order to capture such dynamic phenomena, intravital imaging is an important technique and there is a possibility to provide substantial information that is not available using conventional histological analysis. Multiphoton microscope enable direct, three-dimensional, minimally invasive imaging of biological samples with high spatiotemporal resolution, and now become the main method for intravital imaging studies. Here, we will introduce the latest knowledge obtained by intravital imaging of the skin.

中文翻译：

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皮肤免疫反应的玻璃体内成像。

皮肤中存在各种免疫细胞，并调节皮肤的免疫反应。为了捕获这种动态现象，活体成像是一项重要技术，并且有可能提供使用常规组织学分析无法获得的大量信息。多光子显微镜能够以高时空分辨率对生物样品进行直接，三维，微创成像，现在已成为活体成像研究的主要方法。在这里，我们将介绍通过活体皮肤成像获得的最新知识。