Eggplant or brinjal (Solanum melongena L.) is widely consumed worldwide and thought to trigger allergic reactions in sensitive individuals. So far, no molecular information is available on the allergy-eliciting components of eggplant. In this study, a 17 kDa profilin, Sola m 1, was identified from eggplant by employing an immunoproteomic approach. Based on MALDI-TOF/TOF derived sequences, the full-length cDNA of Sola m 1 was PCR amplified and then cloned. Recombinant (r) Sola m 1 was expressed in E. coli and then purified by metal affinity and gel filtration. rSola m 1 reacted with IgE-antibodies in the sera from all eggplant allergic patients. rSola m 1 also displayed allergenic activity by stimulating histamine release. rSola m 1 was monomeric, and the CD spectra revealed it to be folded with a mixture of α-helices and β-strands. In the melting curve, rSola m 1 exhibited an irreversible denaturation where no refolding took place. Sola m 1 was found to share >80 % sequence identity with Bet v 2, which was further validated by confirming the presence of significant cross-reactivity with Bet v 2 in IgE-inhibition assay. IgE-cross reactivity was also observed between rSola m 1 and profilins from six other foods. In SGF assay, no rSola m 1-derived fragments exhibited IgE-reactivity after prolonged digestion suggesting the association of rSola m 1 with the oral allergy syndromes. Immunofluorescence localization revealed a high abundance of Sola m 1 allergen in eggplant seeds as compared to other edible parts. Taken together, Sola m 1 is the first major eggplant allergen reported in this study, which has the potential of being used as a candidate antigen in component-resolved diagnosis and immunotherapy.

中文翻译：

---

主要茄子（Solanum melongena L.）过敏原的鉴定，克隆和免疫学研究Sola m 1：profilin过敏原家族的新成员。

茄子或茄子（Solanum melongena L.）在世界范围内被广泛食用，并被认为会触发敏感个体的过敏反应。到目前为止，尚无有关茄子引起过敏的分子信息。在这项研究中，通过采用免疫蛋白质组学方法从茄子中鉴定出17 kDa的profilin Sola m 1。根据MALDI-TOF / TOF衍生的序列，PCR扩增Sola m 1的全长cDNA，然后克隆。重组（r）Sola m 1在大肠杆菌中表达，然后通过金属亲和力和凝胶过滤纯化。rSola m 1与所有茄子过敏患者的血清中的IgE抗体反应。rSola m 1还通过刺激组胺释放而显示出变应原活性。rSola m 1是单体的，CD光谱显示它被α-螺旋和β-链的混合物折叠。在熔解曲线中，rSola m 1表现出不可逆的变性，其中没有发生重折叠。发现Sola m 1与Bet v 2共享> 80％的序列同一性，这通过在IgE抑制试验中确认与Bet v 2有明显的交叉反应性进一步证实。在rSola m 1和其他六种食物的脯氨酸蛋白之间也观察到IgE交叉反应性。在SGF分析中，长时间消化后，没有rSola m 1来源的片段显示IgE反应性，表明rSola m 1与口腔过敏综合征相关。免疫荧光定位显示与其他可食用部分相比，茄子种子中的Sola m 1过敏原含量高。综上所述，Sola m 1是该研究报告的第一个主要茄子过敏原，