Gellan gum-based hydrogels display limited cell adhesion ability due to the absence of cell-anchorage points usually present in proteins found in the extracellular matrix (ECM). This issue limits their use in the biomedical field as scaffolds to promote tissue repair. Our work addresses this challenge by investigating the use of polydopamine (pDA) as a bioactive layer to improve the surface and biological properties of gellan gum-based hydrogels cross-linked using carbodiimide chemistry. Upon treatment with a pDA layer, the hydrogel displayed an increase in wettability and swelling properties. This change in physical properties had a direct impact on the biological properties of the scaffolds. Precisely, human adipose-derived stem cells (hASCs) seeded on the pDA coated gellan gum hydrogels displayed larger cell area, increased proliferation rate, and enhanced gene expression of focal adhesion and cytoskeletal proteins. Overall, the findings of this research support the use of pDA coating as a possible approach to improve the biological features of gellan gum-based scaffolds and modulate stem cell morphology and proliferation.

中文翻译：

---

研究聚多巴胺对结冷胶基水凝胶干细胞粘附和增殖的调节作用

基于结冷胶的水凝胶显示出有限的细胞粘附能力，因为细胞外基质 (ECM) 中的蛋白质中通常不存在细胞锚定点。这个问题限制了它们在生物医学领域作为促进组织修复的支架的使用。我们的工作通过研究使用聚多巴胺 (pDA) 作为生物活性层来改善使用碳二亚胺化学交联的结冷胶基水凝胶的表面和生物学特性，从而解决了这一挑战。在用 pDA 层处理后，水凝胶显示出润湿性和溶胀性的增加。这种物理特性的变化对支架的生物学特性有直接影响。准确地说，接种在 pDA 包被的结冷胶水凝胶上的人类脂肪干细胞 (hASC) 显示出更大的细胞面积、增加的增殖率、并增强粘着斑和细胞骨架蛋白的基因表达。总体而言，这项研究的结果支持使用 pDA 涂层作为改善结冷胶基支架的生物学特征和调节干细胞形态和增殖的一种可能方法。