The detailed optimization study for the ultrasound assisted lipase catalyzed synthesis of cinnamyl butyrate under solvent free conditions was described using central composite rotatable design and response surface methodology. The statistical analysis shows that three out of the four variables tested, i.e. molar ratio (A), enzyme loading (B), and ultrasound intensity (D) are found to be statistically significant. The mutual interactions between molar ratio-enzyme loading (AB) and temperature-ultrasound intensity (CD) are also found to be significant along with quadratic interactions of all the parameters (A², B², C², D²). The maximum conversion of 93% was obtained at optimal conditions; molar ratio 2.5 (cinnamyl alcohol:butyric acid); enzyme loading 2.8% of the total mass of reactants; reaction temperature 46 °C and ultrasound intensity 105 W (ultrasound intensity is 7412.68 W/m²). Additionally, hydrodynamic parameters have been determined to estimate the effect of external and intraparticle mass transfer diffusion, which do not show any significant influence on the reaction rate. Reusability study of biocatalyst shows that it can be used up to three cycles with minimal loss of its enzymatic activity.

中文翻译：

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响应面法优化酶催化超声辅助丁酸肉桂酸酯的合成

使用中心复合材料可旋转设计和响应面方法，详细描述了在无溶剂条件下超声辅助脂肪酶催化丁酸肉桂酸酯合成的详细优化研究。统计分析表明，测试的四个变量中的三个，即摩尔比（A），酶负载（B）和超声强度（D）具有统计学意义。还发现摩尔比-酶负载（AB）和温度-超声强度（CD）之间的相互作用以及所有参数（A ²，B ²，C ²，D ^2的二次相互作用）都非常显着。）。在最佳条件下获得了93％的最大转化率；摩尔比2.5（肉桂醇：丁酸）; 酶载量占反应物总质量的2.8％; 反应温度为46°C，超声强度为105 W（超声强度为7412.68 W / m ²）。另外，已经确定了流体动力学参数以估计外部和颗粒内传质扩散的影响，其对反应速率没有显示任何显着影响。生物催化剂的可重复使用性研究表明，它最多可以使用三个循环，而其酶活性损失最小。