Electrospun nanofiber membranes possess high specific surface area with small pores and thus can be developed as wound dressings for absorbing exudate and also preventing bacterial penetration. In this study, hydroxypropyl cellulose (H), chitosan (C) and polyethylene oxide (P) were chosen as membrane materials to increase the hydrophilicity, anti-bacterial property, and yield of nanofibers, respectively. Additionally, graphene (G) was added to enhance the anti-bacterial property of the membranes. As indicated by SEM, the HCP and HCPG solutions (containing H:4.5 wt%, C:4.5 wt%, P:0.75 wt%, without/with G:0.5 wt%) could be electrospun into HCP and HCPG nanofiber membranes with good fiber morphology using a non-toxic solvent system. Further, the membranes were crosslinked by glutaraldehyde vapor to improve the strength. The tensile strength of the membranes was 1.38–1.82 MPa with a swelling ratio up to 1330–1410%. The water vapor transmission rate (WVTR) of wet HCPG membrane was about 3100 g/m²-day, close to the recommended WVTR of wound dressings. The anti-bacterial properties of the membranes were confirmed using three tests against Escherichia coli (Gram-negative bacterium) and Staphylococcus aureus (Gram-positive bacterium). Highly hydrophilic HCP and HCPG membranes prevented the bacterial adherence. The presence of the membranes (especially graphene-embedded HCPG membrane) also greatly reduced bacterial growth. The small pore sizes of HCP and HCPG nanofiber membranes prevented the bacterial penetration to cause infection. Taken together, the HCP and HCPG nanofiber membranes possessed good mechanical properties, appropriate WVTR and high water absorption thus suitable for absorbing wound exudate. Besides, the membranes exhibited nontoxic, anti-fibroblast adhesion and anti-bacterial properties. Therefore, HCP and HCPG nanofiber membranes have the potential to become superior anti-bacterial wound dressings.

中文翻译：

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包埋石墨烯的羟丙基纤维素/壳聚糖/聚环氧乙烷纳米纤维膜作为伤口敷料的增强抗菌性能

电纺纳米纤维膜具有高比表面积和小孔，因此可以作为伤口敷料开发，用于吸收渗出液并防止细菌渗透。在这项研究中，选择羟丙基纤维素（H），壳聚糖（C）和聚环氧乙烷（P）作为膜材料，分别提高了亲水性，抗菌性和纳米纤维的得率。另外，添加石墨烯（G）以增强膜的抗菌性能。如SEM所示，可以将HCP和HCPG溶液（含H：4.5 wt％，C：4.5 wt％，P：0.75 wt％，无/有G：0.5 wt％）电纺成HCP和HCPG纳米纤维膜，具有良好的使用无毒溶剂系统的纤维形态。此外，通过戊二醛蒸汽使膜交联以提高强度。膜的拉伸强度为1.38–1.82 MPa，溶胀率高达1330–1410％。湿HCPG膜的水蒸气透过率（WVTR）约为3100 g / m²天，接近伤口敷料的建议WVTR。使用针对大肠杆菌（革兰氏阴性细菌）和金黄色葡萄球菌的三项测试确认了膜的抗菌特性。（革兰氏阳性细菌）。高度亲水的HCP和HCPG膜可防止细菌粘附。膜（特别是石墨烯嵌入的HCPG膜）的存在也大大减少了细菌的生长。HCP和HCPG纳米纤维膜的小孔径可防止细菌渗透导致感染。综上所述，HCP和HCPG纳米纤维膜具有良好的机械性能，适当的WVTR和高吸水率，因此适合吸收伤口渗出液。此外，该膜还显示出无毒，抗成纤维细胞粘附和抗菌特性。因此，HCP和HCPG纳米纤维膜具有成为优质抗菌伤口敷料的潜力。