Understanding the mechanisms of pyrethroid resistance is essential to the effective management of pesticide resistance in Aphis glycines Matsumura (Hemiptera: Aphididae). We mined putative detoxifying enzyme genes in the draft genome sequence of A. glycines for cytochrome oxidase P450 (CYP), glutathione-S-transferase (GST) and esterases (E4 and carboxylesterases-CES). Aphids from clonal populations resistant to pyrethroids from three sites in Minnesota, USA, were screened against a diagnostic LC99 concentration of either λ-cyhalothrin or bifenthrin and detoxifying enzyme genes expression in survivors was analyzed by qPCR. Their expression profiles were compared relative to a susceptible clonal population. We found 61 CYP (40 full-length), seven GST (all full-length), seven E4 (five full-length) and three CES (two full-length) genes, including 24 possible pseudogenes. The detoxifying enzymes had different expression profiles across resistant aphid populations, possibly reflecting differences in the genetic background and pyrethroid selection pressures as the number of constitutively overexpressed detoxifying enzyme genes was correlated with the level of resistance. Our findings will strengthen the understanding of the pyrethroid resistance mechanisms in A. glycines.

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抗性甘氨酸蚜虫种群中解毒酶基因的组成型和诱导型过度表达的不同模式

了解拟除虫菊酯抗性的机制对于有效管理甘氨酸蚜松村（半翅目：蚜科）的农药抗性至关重要。我们在 A. 甘氨酸基因组序列草图中挖掘了假定的解毒酶基因，用于细胞色素氧化酶 P450 (CYP)、谷胱甘肽-S-转移酶 (GST) 和酯酶（E4 和羧酸酯酶-CES）。针对来自美国明尼苏达州三个地点的对拟除虫菊酯具有抗性的克隆种群的蚜虫，针对 λ-三氯氟氰菊酯或联苯菊酯的诊断性 LC99 浓度进行了筛选，并通过 qPCR 分析了存活者中解毒酶基因的表达。将它们的表达谱与易感克隆群体进行比较。我们发现了 61 个 CYP（40 个全长）、7 个 GST（全长）、7 个 E4（5 个全长）和 3 个 CES（两个全长）基因，包括 24 个可能的假基因。解毒酶在抗性蚜虫种群中具有不同的表达谱，这可能反映了遗传背景和拟除虫菊酯选择压力的差异，因为组成型过度表达的解毒酶基因的数量与抗性水平相关。我们的发现将加强对甘氨酸拟除虫菊酯抗性机制的理解。