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Process development to obtain a cocktail containing cell-wall degrading enzymes with insecticidal activity from Beauveria bassiana
Biochemical Engineering Journal ( IF 3.9 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.bej.2019.107484
Eliana A. Alves , Silvana Schmaltz , Marcus V. Tres , Giovani L. Zabot , Raquel C. Kuhn , Marcio A. Mazutti

Abstract In this work was presented a strategy to produce, to concentrate and to apply a cocktail containing cell-wall degrading enzymes. The novelties and the differences of this study from the others lie in the fact of defining the entire production process of a second generation biopesticide and in addition, an enzyme cocktail will be produced and not a single enzyme as usually studied. Enzymes were produced by solid-state fermentation using Beauveria bassiana fungus and the enzymes concentration was carried out using ultrafiltration membranes. Enzymatic cocktail was used as bioinsecticide for Phereoeca uterella biocontrol. The fermentation increased the enzyme activities in 10 % for exocellulase, 63 % for endocellulase, 60 % for chitinase and 61 % for β-1,3-glucanase. During the step of membrane concentration, was possible to obtain a purification factor of 6.19 for exocellulase, 18.01 for endocellulase, 22.27 for β-1,3-glucanase and 25.07 for chitinase. Finally, the application of permeate and retentate fractions in Phereoeca uterella larvae showed better results in the permeate fraction, results that agreed with the highest enzymatic activity of cocktail.

中文翻译:

从白僵菌中获得含有具有杀虫活性的细胞壁降解酶的混合物的工艺开发

摘要 在这项工作中,提出了一种生产、浓缩和应用含有细胞壁降解酶的混合物的策略。本研究的新颖性和与其他研究的不同之处在于定义了第二代生物农药的整个生产过程,此外,将生产酶混合物,而不是通常研究的单一酶。酶是使用白僵菌通过固态发酵生产的,酶浓缩是使用超滤膜进行的。酶混合物用作生物杀虫剂,用于 Phereoeca utereella 生物防治。发酵使外切纤维素酶的酶活性增加了 10%,内切纤维素酶增加了 63%,几丁质酶增加了 60%,β-1,3-葡聚糖酶增加了 61%。在膜浓缩步骤中,可能获得外切纤维素酶 6.19、内切纤维素酶 18.01、β-1,3-葡聚糖酶 22.27 和几丁质酶 25.07 的纯化因子。最后,在 Phereoeca uterella 幼虫中应用渗透物和滞留物部分在渗透物部分显示出更好的结果,结果与鸡尾酒的最高酶活性一致。
更新日期:2020-04-01
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