Herein, subcritical water extraction (SWE) technology was applied for the recovery of phenolics from chestnut exocarps. Results showed that extracts obtained by SWE had significantly higher extraction yield (Ye), total phenolic contents (TPC), as well as stronger antioxidant (AAs), α-amylase and α-glucosidase inhibitory activities than conventional techniques. The optimal temperature of SWE for reaching the highest Ye, TPC and AAs was 220 °C, while the strongest α-amylase and α-glucosidase inhibitory activities were observed at 120 °C. UPLC-MS/MS, heatmap and radar chart analysis revealed that the phenolic profiles of different extracts varied markedly with extraction methods, and SWE extract at 120 °C had potential application for glycemic control by non-competitively inhibiting α-amylase and/or α-glucosidase. The major components contributed to the inhibitory activities were putative chlorogenic acid and flavonoids, such as rutin and quercetin, etc. These observations suggest that SWE is a promising technology for obtaining bioactive phenolics from chestnut exocarps.

在本文中，亚临界水萃取（SWE）技术用于从板栗外果皮中回收酚类化合物。结果表明，与常规技术相比，通过SWE获得的提取物具有更高的提取率（Ye），总酚含量（TPC）以及更强的抗氧化剂（AAs），α-淀粉酶和α-葡萄糖苷酶抑制活性。达到最高Ye，TPC和AAs的SWE的最佳温度为220°C，而最强的α-淀粉酶和α在120℃下观察到β-葡糖苷酶抑制活性。UPLC-MS / MS，热图和雷达图分析表明，不同提取物的酚类成分随提取方法的变化而显着变化，SWE提取物在120°C下可通过非竞争性抑制α-淀粉酶和/或α的作用来控制血糖-葡糖苷酶。导致抑制活性的主要成分是假定的绿原酸和类黄酮，如芦丁和槲皮素等。这些观察结果表明，SWE是一种从栗子外果皮中获得生物活性酚类的有前途的技术。