Glioma is the prime cause of cancer allied mortality in adolescent people and it accounts about 80% of all malignant tumours. Eugenol is a major bioactive constituent present in the essential oils with numerous pharmacological benefits including nueroprotective activity. The major drawback of eugenol is its extreme volatile property and oxygen sensitivity therefore we increased the efficacy of drug; eugenol by encapsulating with chitosan polymer. Eugenol loaded chitosan polymer (EuCs) was characterized using FTIR, XRD, SEM, HR-TEM analysis and the encapsulation, drug release efficacy was assessed at in vitro condition. The induction of autophagy and anticancer efficacy of EuCs on glioma cells was evaluated with rat C6 glioma cells using MTT assay, acridine orange staining, immunocytochemical analysis of NFκβ protein expression and FLOW cytometric analysis. The anti-metastatic property of Eu-CS was assessed by immunoblotting and RT-PCR analysis of epithelial mesenchymal transition protein expression in EuCs treated rat C6 glioma cells. Our characterization analysis proves that EuCs possess essential physical and functional properties of copolymer to be utilized as a drug. Further the MTT analysis and AO staining confirms even in the presence of oncogenic inducer and autophagic inhibitors, EuCs exhibits apoptotic potency on rat C6 glioma cells. The result of immunocytochemical studies depicts the inhibition of NFκβ protein expression and flow cytometry studies confirm apoptosis induction by EuCs. The inhibition of metastasis by EuCs was proven by the decrease in epithelial mesenchymal transition protein expression in Eu-Cs treated rat C6 glioma cells. Over all our results authentically confirms eugenol loaded chitosan nanopolymer persuasively induces apoptosis and inhibits metastasis in rat C6 glioma cells.

中文翻译：

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丁香酚封装的壳聚糖纳米聚合物通过减轻MMP信号通路对神经胶质瘤C6细胞的凋亡诱导和抗转移活性。

脑胶质瘤是青少年癌症相关死亡率的主要原因，它占所有恶性肿瘤的80％。丁香酚是精油中存在的主要生物活性成分，具有许多药理作用，包括神经保护活性。丁香酚的主要缺点是其极高的挥发性和对氧气的敏感性，因此我们提高了药物的功效。用壳聚糖聚合物包封丁香酚。使用FTIR，XRD，SEM，HR-TEM分析表征了负载丁香酚的壳聚糖聚合物（EuCs），并进行了包封，并在体外条件下评估了药物的释放功效。使用MTT分析，a啶橙染色，NFκB蛋白表达的免疫细胞化学分析和FLOW细胞计数分析。通过免疫印迹和RT-PCR分析EuCs处理的大鼠C6胶质瘤细胞中上皮间质转化蛋白的表达，评估Eu-CS的抗转移特性。我们的表征分析证明，EuCs具有共聚物的基本物理和功能特性，可以用作药物。此外，MTT分析和AO染色证实，即使在存在致癌诱导剂和自噬抑制剂的情况下，EuCs对大鼠C6胶质瘤细胞也具有凋亡作用。免疫细胞化学研究的结果描述了NFκB蛋白表达的抑制作用，流式细胞术研究证实EuCs诱导了细胞凋亡。EuCs对转移的抑制作用由Eu-Cs处理的大鼠C6胶质瘤细胞中上皮间充质过渡蛋白表达的降低证明。在我们所有的结果中，确实证实了负载丁香酚的壳聚糖纳米聚合物可以诱人地诱导大鼠C6胶质瘤细胞凋亡并抑制其转移。