Abstract A simple fluorescent biosensor was developed for the simultaneous determination of the breast cancer-related biomarkers human epidermal growth factor receptor 2 (HER2) and microRNA-21. Black Hole Quencher 2 (BHQ2) labeled DNA1, carboxyl fluorescein (FAM)-modified human epidermal growth factor receptor 2 (HER2) aptamers, and sulfo-cyanine5 carboxylic acid (Cy5)-modified DNA2 were involved. The fluorescent probe was prepared by a one-step procedure. In this sensing system, FAM and Cy5 were close to BHQ2, and therefore, the fluorescent intensity of this proposed probe was weak. However, in the presence of HER2 and microRNA-21, the fluorescent signals were enhanced with increasing concentrations. The detection of HER2 and microRNA-21 was achieved in a simple process. The detection limits for HER2 and microRNA-21 were 0.042 ng mL−1 and 0.048 pM, respectively. In summary, this analytical approach is simple, rapid, cost-effective, low cost, selective, and accurate which are important characteristics in clinical analysis. Therefore, we anticipate that this fluorescent biosensing protocol may have practical applications in clinical measurements to provide early disease diagnosis and monitoring.

中文翻译：

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基于双标记荧光探针同时测定乳腺癌的多种生物标志物

摘要 开发了一种简单的荧光生物传感器，用于同时测定乳腺癌相关生物标志物人表皮生长因子受体 2 (HER2) 和 microRNA-21。Black Hole Quencher 2 (BHQ2) 标记的 DNA1、羧基荧光素 (FAM) 修饰的人表皮生长因子受体 2 (HER2) 适配体和磺基花青 5 羧酸 (Cy5) 修饰的 DNA2 参与其中。荧光探针通过一步法制备。在该传感系统中，FAM 和 Cy5 与 BHQ2 接近，因此，该探针的荧光强度较弱。然而，在存在 HER2 和 microRNA-21 的情况下，荧光信号随着浓度的增加而增强。HER2和microRNA-21的检测是通过一个简单的过程实现的。HER2 和 microRNA-21 的检测限分别为 0.042 ng mL-1 和 0。分别为 048 pm。总之，这种分析方法简单、快速、经济、低成本、选择性和准确是临床分析的重要特征。因此，我们预计这种荧光生物传感协议可能在临床测量中具有实际应用，以提供早期疾病诊断和监测。