Abstract In the present study, one important branch of high-speed capillary electrophoresis (HSCE), short-end CE, was employed for the highly efficient analysis of Porphyromonas gingivalis (Pg), Treponema denticola (Td), and Tannerella forsythia (Tf), which are closely related to the occurrence of periodontal disease. Aiming to realize rapid and simultaneous detection, a 100 bp DNA ladder marker ranging from 100 to 1500 bp was employed as the standard for the optimization of polymer concentration and electric field strength in developed protocol. The results showed that the PCR (polymerase chain reaction) products of Pg (197 bp), Td (311 bp), and Tf (641 bp) were simultaneously identified and quantitated in approximately 90 s using electrophoretic conditions that included 0.4% hydroxyethylcellulose (HEC, 1300 K) and a 200 V/cm electric field with an effective length of separation equal to 2.5 cm. The detected concentrations of the above target samples, determined by a time-modified internal standard route, were 1.08, 1.60, and 2.46 ng/µl, respectively. The present short-end CE strategy featured favorable properties for reproducibility, accuracy and linearity, whose evaluation included the values of relative standard deviation and the correlation coefficient. The current work may provide an effective avenue for the clinical detection of periodontal disease.

中文翻译：

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高速毛细管电泳同时分析多种牙周病原体

摘要 在本研究中，高速毛细管电泳 (HSCE) 的一个重要分支——短端 CE，被用于高效分析牙龈卟啉单胞菌 (Pg)、齿状密螺旋体 (Td) 和连翘单胞菌 (Tf) ，与牙周病的发生密切相关。为了实现快速和同时检测，采用 100 bp 到 1500 bp 的 100 bp DNA 梯形标记作为标准，在开发的协议中优化聚合物浓度和电场强度。结果表明，使用包含 0.4% 羟乙基纤维素 (HEC) 的电泳条件，Pg (197 bp)、Td (311 bp) 和 Tf (641 bp) 的 PCR（聚合酶链反应）产物在大约 90 秒内被同时鉴定和定量。 , 1300 K) 和 200 V/cm 电场，有效分离长度等于 2.5 cm。上述目标样品的检测浓度，通过时间修正内标法测定，分别为 1.08、1.60 和 2.46 ng/µl。目前的短端 CE 策略在重现性、准确性和线性方面具有良好的特性，其评估包括相对标准偏差和相关系数的值。目前的工作可能为牙周病的临床检测提供有效的途径。准确度和线性，其评价包括相对标准偏差和相关系数的值。目前的工作可能为牙周病的临床检测提供有效途径。准确度和线性，其评价包括相对标准偏差和相关系数的值。目前的工作可能为牙周病的临床检测提供有效途径。